In Proteolysis and fragment sequencing, Protein spots may be excised from a two-dimensional protein gel and subjected to a combination of amino acid sequencing and cleavage analyses using the techniques of mass spectrometry and high-pressure liquid chromatography.(a) True(b) FalseI have been asked this question during an interview.My doubt is from Sequence Assembly and Gene Identification topic in division Collecting & Storing Sequences in Laboratory of Bioinformatics

In Proteolysis and fragment sequencing, Protein spots may be excised f

1.	In Proteolysis and fragment sequencing, Protein spots may be excised from a two-dimensional protein gel and subjected to a combination of amino acid sequencing and cleavage analyses using the techniques of mass spectrometry and high-pressure liquid chromatography.(a) True(b) FalseI have been asked this question during an interview.My doubt is from Sequence Assembly and Gene Identification topic in division Collecting & Storing Sequences in Laboratory of Bioinformatics
Answer» The CORRECT answer is (a) True Easy explanation: GENOME regions that encode these sequences can then be identified and the corresponding gene located. A similar METHOD MAY be used to identify the gene that ENCODES a particular protein that has been purified and characterized in the laboratory.

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