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Please help I want explanation as well cause I'm not able to understand log2 1/√8​

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I have 3 groups. 1. Control 2. Disease 3. Treatment. I WANT to lookup the GENE expression btw these groups, compared with control (whether is upregulated or downregulated).

I did real-time qPCR and have ct values. I calculated ∆Ct = Ct[TARGET]-Ct[Housekeeping] ... and ∆∆Ct = (∆Exp.)-(∆Control) and got the -∆∆Ct log-fold-change. It looks all the values are almost same and not MUCH different between the groups.



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