What happens during the ‘elution from the column’ phase in chromatography?(a) Components with greatest affinity elute first(b) Components with least affinity elute first(c) Components elute in a random manner(d) Components elute according to their concentration in the mixtureThe question was asked during a job interview.The origin of the question is Operation of Chromatography topic in division Chromatography of Analytical Instrumentation

What happens during the ‘elution from the column’ phase in chromat

1.	What happens during the ‘elution from the column’ phase in chromatography?(a) Components with greatest affinity elute first(b) Components with least affinity elute first(c) Components elute in a random manner(d) Components elute according to their concentration in the mixtureThe question was asked during a job interview.The origin of the question is Operation of Chromatography topic in division Chromatography of Analytical Instrumentation
Answer» Correct OPTION is (b) Components with least affinity ELUTE first To explain: During the elution phase, different components elute at different TIMES. Components with least affinity elute first.

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