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| 1. |
What would happen when one grows a recombinant bacterium in the bioreactor but forget to add antibiotic to the medium in which the recombinant is growing? |
| Answer» In the absence of antibiotic there will be no pressure on recomb inants to retain the plasmid (containing the gene of our interest). Sinc e, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells. It will thus tend to loose the plasmid. | |