Which of the following cannot be done to reduce ripple in High pressure liquid chromatography?(a) Using bellows(b) Using restrictors(c) Using long nylon tube between pump and column(d) Avoiding the use of the solvent pumpI had been asked this question in an international level competition.I'm obligated to ask this question of High Pressure Liquid Chromatography topic in chapter Chromatography of Analytical Instrumentation

Which of the following cannot be done to reduce ripple in High pressur

1.	Which of the following cannot be done to reduce ripple in High pressure liquid chromatography?(a) Using bellows(b) Using restrictors(c) Using long nylon tube between pump and column(d) Avoiding the use of the solvent pumpI had been asked this question in an international level competition.I'm obligated to ask this question of High Pressure Liquid Chromatography topic in chapter Chromatography of Analytical Instrumentation
Answer» Correct option is (d) Avoiding the use of the solvent pump The best explanation: Solvent pumps have to be USED to reduce PULSES in a solvent flow. When there is a PULSED flow, the efficiency of column DECREASES.

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