Which of the following is incorrect regarding Mass Spectrometry Protein Identification?(a) The proteolysis doesn’t generate a pattern according to molecular weight(b) Proteins can be identified and characterized using MS(c) The proteins from a two dimensional gel system are first digested in situ with a protease(d) Protein spots of interest are excised from the two-dimensional gelThe question was asked by my college professor while I was bunking the class.The doubt is from Technology of Protein Expression Analysis topic in division Functional Genomics & Proteomics of Bioinformatics

Which of the following is incorrect regarding Mass Spectrometry Protei

1.	Which of the following is incorrect regarding Mass Spectrometry Protein Identification?(a) The proteolysis doesn’t generate a pattern according to molecular weight(b) Proteins can be identified and characterized using MS(c) The proteins from a two dimensional gel system are first digested in situ with a protease(d) Protein spots of interest are excised from the two-dimensional gelThe question was asked by my college professor while I was bunking the class.The doubt is from Technology of Protein Expression Analysis topic in division Functional Genomics & Proteomics of Bioinformatics
Answer» Correct answer is (a) The proteolysis doesn’t generate a PATTERN according to molecular weight Easy EXPLANATION: The proteolysis generates a UNIQUE pattern of peptide fragments of VARIOUS MWs, which is termed a peptide fingerprint. The fragments can be ANALYZED with MS, a high-resolution technique for determining molecular masses. Currently, electro-spray ionization MS and matrix-assisted laser desorption ionization (MALDI) MS are commonly used.

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