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Which of the following is not correct about the X-ray Crystallography?(a) In x-ray protein crystallography, proteins need to be grown into large crystals in which their positions are fixed in a repeated, ordered fashion(b) The protein crystals are illuminated with an intense x-ray beam(c) The x-rays are deflected by the electron clouds surrounding the atoms in the crystal producing a regular pattern of diffraction(d) The protein crystals are illuminated with an intense infrared beam |
Answer» The correct option is (d) The protein crystals are illuminated with an intense infrared beam The explanation: The diffraction pattern is composed of thousands of tiny spots recorded on a x-ray film. The diffraction pattern can be converted into an electron density map using a mathematical procedure known as Fourier transform. To interpret a three-dimensional structure from two-dimensional electron density maps requires solving the phases in the diffraction data. |
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