1.

Which of the following is the volume of mobile phase required to make a solute band move from the point of injection through the column to the detector?(a) Dead volume(b) Retention volume(c) Void volume(d) Adjusted retention volumeThis question was posed to me in examination.Question is taken from Chromatographic Behaviour of Solutes topic in section Chromatography of Analytical Instrumentation

Answer»

The correct OPTION is (b) Retention volume

To explain I would SAY: Retention volume is the volume of mobile phase REQUIRED to make a solute band move from the POINT of INJECTION through the column to the detector.



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