Which of the following is untrue about Shotgun Sequencing?(a) When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to overlap(b) When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to scrutinize(c) In a new whole shotgun approach, Celera Genomics is sequencing both ends of DNA fragments of short (2 kb), medium (10 kb), and long (BAC or >100 kb) lengths(d) A large number of reads are then assembled by computerThis question was addressed to me by my college director while I was bunking the class.Query is from DNA & Genomic Sequencing topic in portion Collecting & Storing Sequences in Laboratory of Bioinformatics

Which of the following is untrue about Shotgun Sequencing?(a) When DNA

1.	Which of the following is untrue about Shotgun Sequencing?(a) When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to overlap(b) When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to scrutinize(c) In a new whole shotgun approach, Celera Genomics is sequencing both ends of DNA fragments of short (2 kb), medium (10 kb), and long (BAC or >100 kb) lengths(d) A large number of reads are then assembled by computerThis question was addressed to me by my college director while I was bunking the class.Query is from DNA & Genomic Sequencing topic in portion Collecting & Storing Sequences in Laboratory of Bioinformatics
Answer» Correct answer is (b) When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to scrutinize The explanation: A controversy has arisen as to whether or not the above shotgun sequencing strategy can be applied to genomes with repetitive sequences such as those likely to be encountered in sequencing the human genome. This method has been used to assemble the genome of the fruit fly Drosophila melanogaster after REMOVAL of the most HIGHLY repetitive regions and also to assemble a SIGNIFICANT proportion of the human genome.

Discussion

No Comment Found

Related InterviewSolutions

Which of the given statements is incorrect?(a) Two tandem copies of a gene are produced while Proteins with new functions are produced(b) Proteins with new functions are produced by a gene duplication event(c) Assortment and reassortment of protein domains takes place in individual genomes(d) In no case the two duplicated genes both undergo changeI got this question by my school teacher while I was bunking the class.Query is from Comparative Genomics topic in section Collecting & Storing Sequences in Laboratory of Bioinformatics
Which of the given statements is untrue?(a) Sequence and other data files that contain non-ASCII characters also may not be transferred correctly from one machine to another and may cause unpredictable behavior of the communications software(b) The ASCII mode is useful for transferring text files, and the binary mode is useful for transferring compressed data files, which also contain non-ASCII characters(c) ASCII and binary modes cannot be set by the user(d) Most sequence analysis programs also require not only that a DNA or protein sequence file be a standard ASCII file, but also that the file be in a particular format such as the FASTA formatI had been asked this question by my college director while I was bunking the class.I would like to ask this question from Sequence Formats & Computer Storage of Sequences in section Collecting & Storing Sequences in Laboratory of Bioinformatics
cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).(a) True(b) FalseThe question was posed to me by my college professor while I was bunking the class.My question is based upon Sequencing cDNA Libraries of Expressed Genes, Submission of Sequences to the Databases in portion Collecting & Storing Sequences in Laboratory of Bioinformatics
The combined mixture of all labeled DNA fragments is electrophoresed to _____ the fragments by______ and the ladder of fragments is scanned for the presence of each of the four labels.(a) separate, size(b) separate, pH(c) assimilate, pH(d) assimilate, sizeI got this question in an international level competition.This intriguing question originated from DNA & Genomic Sequencing topic in section Collecting & Storing Sequences in Laboratory of Bioinformatics
_____ of the human genome comprises one particular family of the SINE Element, designated Alu (1.2 million copies).(a) 10%(b) 20%(c) 60%(d) 40%The question was posed to me by my school principal while I was bunking the class.Question is taken from Genome Anatomy in section Collecting & Storing Sequences in Laboratory of Bioinformatics
Vertebrate chromosomes have long (>300 kb) regions of distinct GC richness, repeat content, and gene density, designated isochores in a model of genome organization proposing that genomes are made up of distinct segments of unique composition.(a) True(b) FalseThe question was asked in an international level competition.This question is from Genome Anatomy topic in division Collecting & Storing Sequences in Laboratory of Bioinformatics
More than _____ of the human genome consists of interspersed repetitive sequences derived from TEs (transposable elements).(a) one-third(b) one-eighth(c) one-fifth(d) halfThis question was addressed to me during an online interview.My doubt stems from Genome Anatomy topic in portion Collecting & Storing Sequences in Laboratory of Bioinformatics
Minisatellites are made up of repeat units of up to ____ and microsatellites compose of repeat units of ____ or less.(a) 25 bp, 10 bp(b) 70 bp, 6 bp(c) 80 bp, 9 bp(d) 25 bp, 4 bpI had been asked this question during an interview for a job.My enquiry is from Genome Anatomy in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics
Annotation involves identifying open reading frames in the genome sequence.(a) True(b) FalseThis question was addressed to me during an interview.The origin of the question is Genome Anatomy in portion Collecting & Storing Sequences in Laboratory of Bioinformatics
Which of the following is incorrect about ENTREZ?(a) There is no simple way to find the correct sequence without manually checking the information provided in each sequence, but this usually takes longer time(b) Before leaving ENTREZ, it is often useful to check for sequence database entries that are similar to the one of interest, called “neighbors” by ENTREZ(c) The expanded query searches other database entries of interest, such as the same protein in another organism, a large chromosomal sequence that includes the gene, or members of the same gene family(d) While visiting the site, note that ENTREZ has been adapted to search through a number of other biological databases, and also through Medline, and these searches are available from the initial ENTREZ Web pageThis question was posed to me in quiz.My question comes from Using the Database Access Program ENTREZ topic in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment