Which of the following is untrue about the drawbacks of SAGE?(a) One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification(b) Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all(c) Correctly sequenced SAGE tag always corresponds to several genes(d) The drawback with this approach is the sensitivity to sequencing errorsThe question was asked by my school principal while I was bunking the class.I need to ask this question from Comparison of SAGE and DNA Microarrays topic in portion Functional Genomics & Proteomics of Bioinformatics

Which of the following is untrue about the drawbacks of SAGE?(a) One o

1.	Which of the following is untrue about the drawbacks of SAGE?(a) One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification(b) Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all(c) Correctly sequenced SAGE tag always corresponds to several genes(d) The drawback with this approach is the sensitivity to sequencing errorsThe question was asked by my school principal while I was bunking the class.I need to ask this question from Comparison of SAGE and DNA Microarrays topic in portion Functional Genomics & Proteomics of Bioinformatics
Answer» Correct option is (c) CORRECTLY sequenced SAGE tag ALWAYS corresponds to SEVERAL genes Easy explanation: To improve the sensitivity and SPECIFICITY of SAGE detection, the lengths of the tags need to be increased for the technique. There are some comprehensive software tools for SAGE analysis viz. SAGEmap, SAGEXPROFILER.

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