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Which of the following steps takes place after injection of feed in Column chromatography?(a) Detection of components(b) Separation in the column(c) Elution from the column(d) Collection of eluted componentI got this question by my school principal while I was bunking the class.This question is from Operation of Chromatography in section Chromatography of Analytical Instrumentation

Answer»

The CORRECT choice is (b) SEPARATION in the column

The best I can explain: The OPERATION that occurs after the INJECTION of feed is a separation in the column. After that, elution from the column and detection of COMPONENTS takes place.



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