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1.

Which is the inducer of enzyme invertase?(a) Starch(b) Sucrose(c) Galactosidases(d) Fatty acidsThis question was posed to me in quiz.My query is from Large in chapter An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

The correct answer is (b) SUCROSE

To explain: INVERTASE is an enzyme that catalyzes the hydrolysis (breakdown) of sucrose. The resulting mixture of fructose and glucose is called inverted sugar SYRUP. For industrial use, invertase is usually derived from yeast. The inducer compounds are expensive and their handling (sterilization, addition at specific TIME) ALSO is quite difficult.

2.

Facultative autotrophs can grow under heterotrophic condition.(a) True(b) FalseThis question was addressed to me during an online exam.My enquiry is from Cell Nutrients in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

The correct option is (a) True

Explanation: FACULTATIVE autotrophs normally grow under autotrophic conditions; however, they can grow under HETEROTROPHIC conditions in the absence of CO2 and INORGANIC ENERGY sources.

3.

Micronutrient which is important in transport of sugar, synthesis of enzymes and cell division is?(a) Boron(b) Potassium(c) Calcium(d) PhosphorusI had been asked this question by my college director while I was bunking the class.Origin of the question is Cell Nutrients in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Correct choice is (a) Boron

Easy explanation: Boron (B) is a micronutrient critical to the growth and health of all crops. It is a COMPONENT of plant cell walls and reproductive structures. It is a mobile nutrient within the soil, meaning it is prone to MOVEMENT within the soil. Because it is REQUIRED in small amounts, it is important to deliver B as evenly as possible across the field. Boron plays a key role in a diverse range of plant functions including cell wall formation and stability, maintenance of structural and functional integrity of biological membranes, movement of SUGAR or energy into growing parts of plants, and pollination and seed set. Adequate B is also required for EFFECTIVE nitrogen fixation and nodulation in legume crops.

4.

Viruses contain lipids.(a) True(b) FalseI got this question during an interview.Enquiry is from Cell Nutrients topic in chapter An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right option is (a) True

The best explanation: VIRUSES with a LIPOPROTEIN sheath may CONTAIN 25% lipid. Composition as a dry weight % INCLUDES <1.

5.

Which enzyme was first produced industrially?(a) Bacteria enzyme(b) Yeast enzyme(c) Fungal enzyme(d) StreptomycesI had been asked this question at a job interview.The doubt is from Large topic in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right option is (c) FUNGAL ENZYME

Explanation: Microbial enzymes have been utilized for many centuries WITHOUT knowing them fully. The FIRST enzyme produced industrially was taka-diastase (a fungal AMYLASE) in 1896, in United States. It was used as a pharmaceutical agent to cure digestive disorders.

6.

What is the method of screening?(a) To improve the microbial strain(b) To improve the growth of an enzyme(c) To choose appropriate microorganisms for the desired enzyme(d) To determine the optimum conditions for growth of microbesThe question was asked in an interview for job.My question comes from Large topic in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Correct answer is (C) To choose appropriate microorganisms for the DESIRED enzyme

For explanation I would say: Screening is one of the KEY optimization parameters to understand the DYNAMICS of the process, ALLOWING for rapid understanding and enhancement of process conditions by analyzing culture metabolic rates and metabolites.

7.

Which of the following is the key element in the regulation of cell metabolism?(a) Hydrogen(b) Phosphorus(c) Oxygen(d) NitrogenThis question was addressed to me during an interview.My question is taken from Cell Nutrients topic in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Correct choice is (b) Phosphorus

Easy explanation: The MAIN role of potassium is to provide the ionic environment for METABOLIC processes in the cytosol, and as such functions as a REGULATOR of various processes including growth regulation. Plants require potassium ions (K^+) for protein SYNTHESIS and for the opening and closing of stomata, which is regulated by proton pumps to make surrounding guard cells either turgid or flaccid. Phosphorus constitutes about 3% of cell DRY weight and is present in nucleic acids and in the cell wall of some gram-positive bacteria such as teichoic acids. Phosphorus is a key element in the regulation of cell metabolism.

8.

Which of the following is true for single cell protein?(a) Algae cannot be used in single cell protein(b) It is produced through fermentation(c) It does not contain carbohydrates and vitamins(d) Its utilization increases environmental pollutionThis question was addressed to me during an online interview.I would like to ask this question from Large in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right option is (b) It is produced through fermentation

Best explanation: Single-cell proteins develop when microbes FERMENT waste MATERIALS (including wood, straw, cannery, and food-processing wastes, residues from alcohol production, hydrocarbons, or human and animal excreta). The problem with extracting single-cell proteins from the wastes is the dilution and cost. They are found in very low concentrations, usually less than 5%. Engineers have developed ways to increase the concentrations including centrifugation, flotation, precipitation, coagulation, and filtration, or the USE of semi-permeable membranes.

9.

The production of enzyme is mostly carried out by?(a) Batch fermentation(b) Continuous fermentation(c) Fed-batch fermentation(d) Semi-batch fermentationThe question was asked in an interview for job.The origin of the question is Large in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer» RIGHT answer is (a) Batch fermentation

Explanation: The MEDIUM can be sterilized by employing batch or continuous sterilization techniques. The fermentation is started by INOCULATING the medium. The growth conditions (PH, temperature, O2 supply, nutrient addition) are maintained at optimal levels. The froth FORMATION can be minimised by adding antifoam agents. The production of enzymes is mostly carried out by batch fermentation and to a lesser extent by continuous process.
10.

In Upstream processes, process development increases the mRNA hydrolysis and proteolysis?(a) True(b) FalseI had been asked this question in examination.My enquiry is from Large topic in chapter An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Correct answer is (b) False

For explanation: PROCESS development is typically performed after GENETIC modification of the SOURCE organism, and involves the modification of the culture medium and GROWTH conditions. In many cases, process development aims to reduce mRNA hydrolysis and PROTEOLYSIS.

11.

Which type of fermentation is used for large scale manufacturing of enzymes?(a) Solid-state fermentation(b) Submerged fermentation(c) Solid-Gas state fermentation(d) Gas-state fermentationThis question was addressed to me during a job interview.My doubt stems from Large in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

The correct choice is (b) SUBMERGED FERMENTATION

The best explanation: Submerged fermentation is a method of manufacturing biomolecules in which enzymes and other reactive compounds are submerged in a LIQUID such as alcohol, oil or a NUTRIENT broth. The process is used for a variety of PURPOSES, mostly in industrial manufacturing.

12.

The Macronutrients concentration needed is __________(a) Greater than 10^-2 M(b) Less than 10^-2 M(c) Greater than 10 ^-4 M(d) Less than 10^-4 MI have been asked this question in unit test.I'm obligated to ask this question of Cell Nutrients topic in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right answer is (c) GREATER than 10 ^-4 M

For explanation I would say: Macronutrients are needed in concentrations larger than 10^-4 M. Carbon, nitrogen, OXYGEN, hydrogen, SULFUR, phosphorus, Mg^2+, and K^+ are MAJOR macronutrients.

13.

Which of the following purified enzyme is used in pharmaceutical industry?(a) Subtilisin(b) Novozym-435(c) Bromelain(d) AsparaginaseI have been asked this question during an internship interview.My question comes from Large topic in chapter An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»
14.

Which of the following does not produces “Proteases”?(a) Bacillus(b) Rhizopus(c) Mucor(d) Bacillus coagulansI have been asked this question during an online exam.This key question is from Large in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right OPTION is (d) Bacillus coagulans

Easy EXPLANATION: A process in which a MUTANT atypical Bacillus coagulans strain is cultivated in a growth medium free of xylose. The invention RELATES to glucose ISOMERASE, to methods for the production of this enzyme and to enzymatic isomerization of glucose to fructose with glucose isomerase.

15.

Which of the following is not a defined media?(a) Glucose(b) Magnesium chloride(c) Calcium chloride(d) Phenyl acetic acidI have been asked this question during an internship interview.My doubt stems from Cell Nutrients topic in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer» RIGHT choice is (d) Phenyl acetic acid

To explain: Defined MEDIA contain specific amounts of pure chemical compounds with known chemical compositions. A medium containing glucose, (NH4)2SO4, KH2PO4, and MgCl2 is a defined medium. Complex media contain natural compounds whose chemical composition is not exactly known. Complex medium used in penicillin fermentation includes Phenyl acetic acid by continuous feed in a CONCENTRATION of about 0.5-0.8% of total (g/liter).
16.

Energy is measured in ________(a) Micronutrient(b) Macronutrient(c) Calories(d) KilogramI got this question by my school principal while I was bunking the class.Query is from Cell Nutrients in section An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

The correct option is (c) CALORIES

Best explanation: A CALORIE is a unit that is USED to measure ENERGY. The Calorie you see on a food package is actually a kilocalorie, or 1,000 calories. A Calorie (kcal) is the amount of energy needed to RAISE the temperature of 1 kilogram of water 1 degree Celsius.

17.

Which of the following ion is required by the ribosomes?(a) H^+(b) Mg^2+(c) Mn^2+(d) Ca^2+I have been asked this question during an interview.I want to ask this question from Cell Nutrients topic in section An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right answer is (B) Mg^2+

Easiest explanation: Magnesium, SERVES as a structural component and is involved as a cofactor in many ENZYMATIC reactions. Magnesium is a component of the CHLOROPHYLL structure. Magnesium is required to maintain ribosome integrity. Ribosomes SPECIFICALLY require Mg^2+ ions.

18.

The minerals involved in water-splitting reaction during photosynthesis are ___________(a) Manganese and chloride(b) Magnesium and sulfur(c) Magnesium and potassium(d) Magnesium and phosphorusI got this question in semester exam.I'm obligated to ask this question of Cell Nutrients in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

The correct answer is (a) MANGANESE and chloride

The best I can explain: Water splitting is catalyzed by the oxygen-evolving complex (OEC) of protein complex photosystem II (PSII), producing dioxygen gas, protons and electrons. O (2) is released into the atmosphere, sustaining all aerobic life on earth; PRODUCT protons are released into the thylakoid lumen, augmenting a proton concentration GRADIENT across the MEMBRANE; and photo-energized electrons pass to the rest of the electron-transfer PATHWAY. The OEC contains four manganese ions, one calcium ion and (almost certainly) a chloride ion.

19.

Complex carbohydrates which make up cell wall in plants are?(a) Lactose(b) Fructose(c) Cellulose(d) SucroseThe question was asked in an interview for job.Origin of the question is Cell Nutrients in chapter An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

The correct option is (c) Cellulose

The best EXPLANATION: Cellulose is a very important polysaccharide because it is the most abundant organic COMPOUND on earth. Cellulose is a major component of tough cell walls that surround plant CELLS, and is what makes plant stems, leaves, and BRANCHES so STRONG.

20.

What is “EDTA”?(a) Magnesium calcium edentate(b) Nitrogen phosphorus edentate(c) Sodium calcium edentate(d) Magnesium phosphorus edentateThe question was asked by my college director while I was bunking the class.The query is from Cell Nutrients in section An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Correct answer is (c) Sodium calcium edentate

The explanation: A specific SALT of EDTA, known as sodium calcium edetate, is used to BIND metal ions in the practice of CHELATION therapy, e.g., for treating mercury and lead poisoning. It is used in a similar MANNER to remove excess iron from the body. EDTA is a chelating agent and a chelating agent is a substance whose molecules can FORM several bonds to a single metal ion. In other words, a chelating agent is a multidentate ligand.

21.

Which of the following can grow in absence of CO2 or carbon sources?(a) Autotrophs(b) Mixotrophs(c) Chemoautotrophs(d) Facultative autotrophsThis question was addressed to me during an interview for a job.This is a very interesting question from Cell Nutrients in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer» CORRECT answer is (d) Facultative AUTOTROPHS

Best explanation: Facultative autotrophs NORMALLY grow under autotrophic conditions; however, they can grow under heterotrophic conditions in the absence of CO2 and INORGANIC energy sources.
22.

What do you mean by “Nutrient repression”?(a) Inhibition of unwanted enzyme production(b) Production of unwanted enzymes(c) Inhibition of cell nutrients(d) Production of wasteThis question was posed to me during an interview.Asked question is from Large topic in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer» RIGHT answer is (a) Inhibition of UNWANTED enzyme production

For explanation: The native metabolism of microorganism is so devised that there occurs no production of unnecessary enzymes. In other words, the MICROORGANISMS do not synthesize enzymes that are not required by them, since this is a wasteful exercise. The inhibition of unwanted enzyme production is done by nutrient repression. The nutrients may be CARBON, NITROGEN, phosphate or sulfate suppliers in the growth medium. For large scale production of enzymes, nutrient repression must be overcome.
23.

Which of the following is not included in immobilization process?(a) Absorption(b) Adsorption(c) Entrapment(d) AffinityI have been asked this question in an interview.Origin of the question is Large in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer» RIGHT answer is (a) Absorption

The explanation is: DESPITE their excellent catalytic capabilities, enzymes and their properties must be improved prior to industrial implementation in many cases. Some aspects of enzymes that must be improved prior to implementation are stability, activity, inhibition by reaction PRODUCTS, and selectivity towards non-natural substrates. This may be accomplished through immobilization of enzymes on a solid material, such as a porous support. Immobilization of enzymes greatly simplifies the recovery process, enhances process control, and reduces operational costs. Many immobilization TECHNIQUES exist, such as adsorption, COVALENT binding, affinity, and entrapment.
24.

The inactive cells are advantageous for enzyme activity.(a) True(b) FalseThe question was asked in an online interview.This interesting question is from Large topic in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

The correct choice is (a) True

To ELABORATE: In some CASES, it may be more advantageous to use inactive (dead or resting) CELLS with the desired ENZYME activity in IMMOBILIZED form. This approach eliminates costly enzyme separation and purification steps and is therefore economically more feasible.

25.

Which of the following components is not a Micronutrient?(a) Zn^2+(b) Ca^2+(c) Na^+(d) Mg^2+The question was posed to me in a job interview.The doubt is from Cell Nutrients in chapter An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right choice is (d) Mg^2+

To elaborate: Elements used in LARGE quantities by the plant are termed macronutrients, which can be further defined as primary or secondary.The primary nutrients include NITROGEN (N), phosphorus (P), and potassium (K).These elements contribute to plant nutrient content, function of plant enzymes and biochemical processes, and integrity of plant cells.Deficiency of these nutrients contributes to reduced plant growth, health, and yield; thus they are the three most important nutrients supplied by fertilizers.The secondary nutrients include calcium (Ca), MAGNESIUM (Mg), and sulfur (S).

26.

System of reaction in which no reactant leaves reaction mixture is termed as ___________(a) Open system(b) Closed system(c) Semi-open system(d) Partially closed systemI had been asked this question by my school principal while I was bunking the class.I would like to ask this question from Large in section An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer» RIGHT ANSWER is (b) Closed system

To EXPLAIN: One of the fundamental foundations of bioprocess system design is the use of “closed” systems for PRODUCTION. As a key element of Good Manufacturing Practice (GMP) compliance, closed systems and equipment are utilized to PREVENT contamination of the product.
27.

Cellulase is used in which type of industry?(a) Food industry(b) Paper industry(c) Biofuel industry(d) Chemical industryThis question was posed to me by my school principal while I was bunking the class.My enquiry is from Large topic in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

The CORRECT choice is (c) BIOFUEL industry

For explanation I would SAY: Cellulase is used in biofuel industry and its application is class of enzymes that degrade CELLULOSE to GLUCOSE monomers.

28.

Which of the following is not an upstream process?(a) Selection of a suitable enzyme(b) Process development(c) Concentration and primary purification of enzymes(d) Large scale productionI got this question in examination.I'm obligated to ask this question of Large in section An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right answer is (c) CONCENTRATION and PRIMARY purification of enzymes

To explain: Downstream processes are those that contribute to separation or purification of enzymes. Depending on the intended use of the enzyme, different levels PURITY is required. For EXAMPLE, enzymes used for diagnostic purposes must be separated to a higher purity than bulk industrial enzymes to prevent catalytic activity that provides erroneous results.

29.

Which device is used to measure the amount of energy stored in foods?(a) Rheometer(b) Adiabatic calorimeter(c) Bomb calorimeter(d) Heat flow calorimeterThis question was posed to me in an interview for internship.This interesting question is from Cell Nutrients topic in portion An Overview of Biological Basics & Enzymes of Bioprocess Engineering

Answer»

Right option is (c) BOMB calorimeter

To explain: Complex organic food MOLECULES such as sugars, fats, and proteins are rich sources of ENERGY for CELLS because much of the energy used to form these molecules is literally stored within the chemical bonds that hold them TOGETHER. Scientists can measure the amount of energy stored in foods using a device called a bomb calorimeter. With this technique, food is placed inside the calorimeter and heated until it burns. The excess heat released by the reaction is directly proportional to the amount of energy contained in the food.