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Which of the following is not a defined media?(a) Glucose(b) Magnesium chloride(c) Calcium chloride(d) Phenyl acetic acidI have been asked this question during an internship interview.My doubt stems from Cell Nutrients topic in division An Overview of Biological Basics & Enzymes of Bioprocess Engineering |
Answer» RIGHT choice is (d) Phenyl acetic acid To explain: Defined MEDIA contain specific amounts of pure chemical compounds with known chemical compositions. A medium containing glucose, (NH4)2SO4, KH2PO4, and MgCl2 is a defined medium. Complex media contain natural compounds whose chemical composition is not exactly known. Complex medium used in penicillin fermentation includes Phenyl acetic acid by continuous feed in a CONCENTRATION of about 0.5-0.8% of total (g/liter). |
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