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1.

What is the generation time of a bacterial population that increases from 10,000 cells to 10,000,000 cells in four hours of growth?(a) 24 minutes(b) 30 minutes(c) 34 minutes(d) 60 minutesThe question was asked in an internship interview.I would like to ask this question from Data Analysis topic in chapter Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right option is (a) 24 minutes

The explanation is: The generation time is the time interval required for the CELLS (or population) to divide.

G (generation time) = (time, in minutes or HOURS)/n(number of generations)

G = t/n

t = time interval in hours or minutes

B = number of bacteria at the beginning of a time interval

b = number of bacteria at the end of the time interval

n = number of generations (number of times the cell population doubles during the time interval)

b = B x 2^n (This equation is an expression of growth by binary FISSION)

Solve for n:

LOGB = logB + nlog2

n = \(\frac{log_b – log_B}{log2}\)

n = \(\frac{log_b – log_B}{.301}\)

n = 3.3 logb/B

G = t/n

2.

From the following table which one is the dependent and independent variable and what type of graph would you choose for this data?(a) Dependent – Bubbles per minute Plant A, Independent- Bubbles per minute Plant B, Graph- Pie chart(b) Dependent – Depth (in meters), Independent- Bubbles per minute Plant A, Graph- Line plot(c) Dependent – Depth (in meters), Independent- Bubbles per minute Plant B, Graph- Line plot(d) Dependent – Bubbles per minute Plant A and Plant B, Independent- Depth (in meters), Graph- Line plotThe question was posed to me in exam.Question is from Graph Paper with Logarithmic Coordinates in chapter Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct choice is (d) Dependent – Bubbles per minute Plant A and Plant B, Independent- Depth (in meters), Graph- Line plot

Easiest explanation: Dependent VARIABLE-

Bubbles per minute – the amount of bubbles produced will depend on how DEEP the plant is in the water. The variable that DEPENDS on another variable is the dependent variable.

Independent variable-

The depth in meters – you are controlling the depths at which you place the plants and observing what happens to the number of bubbles. Typically the variable you have CONTROL over is the independent variable.

Graph-

Line – we are measuring the change in bubble production over time. (Why not a bar? – There are some gradual changes that may be lost depending on our scale. Also, LINES are often more SIMPLE to read and less confusing).

3.

In the case of Rota meter, which is used for measuring the water flow rate?(a) Only one variable is involved, i.e. independent variable (the Rota meter reading)(b) Only two variables are involved: one independent variable(the Rota meter reading) and one dependent variable (the water flow rate)(c) Only two variables are involved: one independent variable(the water flow rate) and one dependent variable (the Rota meter reading)(d) Only one variable is involved, i.e. independent variable (the water flow rate)This question was posed to me in class test.This interesting question is from Data Analysis topic in section Presentation and Analysis of Data of Bioprocess Engineering

Answer» CORRECT ANSWER is (b) Only two variables are INVOLVED: one independent variable(the Rota meter READING) and one dependent variable (the water flow rate)

Explanation: Rota meter is a device which measures flow rate. This is done by allowing the cross-sectional area the fluid travels through to vary, causing a measurable effect, i.e. water flow rate is dependent on Rota meter.
4.

Refer to Q9 and calculate the variance of each data set?(a) (1239,8.0)(b) (1823,8.9)(c) (8.9,1823)(d) (8.0,1239)I have been asked this question by my college director while I was bunking the class.My doubt is from Graph Paper with Logarithmic Coordinates topic in portion Presentation and Analysis of Data of Bioprocess Engineering

Answer»

The CORRECT answer is (b) (1823,8.9)

The explanation: In VARIANCE there is SMALL culture and bulk culture.

Small culture-

The SUM of values in the (X-x̅)^2 column = Ƹ(X-x̅)^2 = 16,403

Since there are 10 samples in this data set, n=10. Placing these values into the formula for CALCULATING the variance yields,

Variance = \(\frac{Ƹ(X-x̅)^2}{n-1} = \frac{16.403}{10-1}\)

=\(\frac{16.403}{9}\)

=1823

Bulk culture-

The sum of values in the (X-x̅)^2 column = Ƹ(X-x̅)^2 = 80

Since there are 10 samples in this data set, n=10. Placing these values into the formula for calculating the variance yields,

Variance = \(\frac{Ƹ(X-x̅)^2}{n-1} = \frac{80}{10-1}\)

= \(\frac{80}{9}\)

= 8.9

5.

You will read the meniscus reading as you measure the level at the horizontal center or inside part of the meniscus.(a) True(b) FalseThe question was asked in semester exam.Question is from Errors in Data and Calculations in division Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct answer is (a) True

The explanation: Place the GRADUATED cylinder on a flat surface and view the height of the liquid in the cylinder with your eyes directly level with the liquid. The liquid will TEND to curve DOWNWARD. This curve is called the meniscus. Always read the MEASUREMENT at the BOTTOM of the meniscus.

6.

From the below representation of growth phase, which is the missing growth phase?(a) Exponential Growth Phase(b) Final Lag Phase(c) Initial Log Phase(d) Final Log phaseThis question was posed to me by my college director while I was bunking the class.I would like to ask this question from Data Analysis in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right option is (a) Exponential GROWTH Phase

For explanation I would say: The Log/ Exponential Growth phase is a period characterized by cell doubling. If growth is not limited, doubling will continue at a constant rate so both the number of CELLS and the rate of population increase doubles with each consecutive TIME period. For this type of exponential growth, plotting the natural logarithm of cell number against time produces a STRAIGHT LINE.

7.

Bacillus cereus divides every 30 minutes. You inoculate a culture with exactly 100 bacterial cells. After 3 hours, how many bacteria are present and what is the generation number and time?(a) (24,000), (3 generations), (30 minutes per generation)(b) (64,000), (6 generations), (30 minutes per generation)(c) (24,000), (6 generations), (60 minutes per generation)(d) (64,000), (3 generations), (60 minutes per generation)I had been asked this question in a national level competition.I want to ask this question from Graph Paper with Logarithmic Coordinates in section Presentation and Analysis of Data of Bioprocess Engineering

Answer» CORRECT choice is (b) (64,000), (6 GENERATIONS), (30 minutes per generation)

The best I can explain: Number of cells:

In 3 hours, B.cereus will divide 6 times. THEREFORE, n=6.

2^6 = 64 or 2 × 2 × 2 × 2 × 2 × 2.

100×64=64,000cells.

Number of generations:

Number of generations = [log (cells at the end of incubation)]-[log (cells at the beginning of incubation)]/0.301.

Therefore, [(log 6400) – (log 100)]/0.301 = (3.81-2)/0.301 = 6 generations.

Generation time:

60 min×3 hours / 6 generations = 30 minutes per generation.
8.

What is the rounding off of a number?(a) To change a number to its nearest prime number(b) To change a number to its nearest odd number(c) To change a number to its nearest whole number(d) To change a number to its nearest even numberI had been asked this question during an interview.This interesting question is from Errors in Data and Calculations topic in division Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct answer is (C) To change a number to its nearest whole number

For explanation: To reduce successively the number of digits to the RIGHT of the decimal point of a MIXED number by dropping the final DIGIT and adding 1 to the next preceding digit if the dropped digit was 5 or GREATER, or leaving the preceding digit unchanged if the dropped digit was 4 or less.

9.

What type of surface you would observe in the case of mercury with glass?(a) Concave meniscus(b) Convex meniscus(c) Contact angle = 180°(d) Contact angle = 0°I got this question in an online quiz.This is a very interesting question from Errors in Data and Calculations in portion Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right OPTION is (b) Convex meniscus

Easiest EXPLANATION: A convex meniscus occurs when the molecules have a stronger ATTRACTION to each other than to the container and Mercury does not wet GLASS – the COHESIVE forces within the drops i.e., bond between the molecules of mercury are stronger than the adhesive forces between the drops and glass.

10.

The manipulated variable levels Continuous (i.e. time in years) or Clumped into groups( i.e. 0-5 years, 6-10 years) is represented as _________(a) Continuous- Scatter plot/line, Clumped- Histogram(b) Continuous- Histogram, Clumped- Scatter plot/line(c) Continuous- Pie chart, Clumped- Scatter plot/line(d) Continuous- Pie chart, Clumped- HistogramThis question was addressed to me in final exam.Question is taken from Graph Paper with Logarithmic Coordinates in portion Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct answer is (a) Continuous- Scatter plot/LINE, Clumped- Histogram

Explanation: HISTOGRAM :

Histograms are similar to bar graphs except the data represented in histogram is usually in groups of continuous numerical (quantitative) data. In this case, the bars do touch. Histograms are often used to show FREQUENCY data.

Example- Average MEAN temperature between the years 1900 and 2000

SCATTER- PLOT:

The points are plotted on the grid, but they are not JOINED point to point. A best fit line may be added to a scatter plot to show a trend. Line graphs are only used when both variables are quantitative. These graphs are useful for showing if a CORRELATION exists between two variables, especially when it is not possible to alter either of the variables (i.e. in descriptive studies).

Example – Reaction rate at various enzyme concentrations.

11.

Is the Power law related to the straight line in the log-log plot?(a) True(b) FalseThis question was posed to me during an online exam.My question comes from Graph Paper with Logarithmic Coordinates topic in chapter Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct answer is (a) True

The explanation is: A straight LINE on log-log graph paper corresponds to the equation:

Y = Bx^A

In y = In B + A In x

If A is positive, y = 0 when x = 0, If A is negative, when x = 0, y is infinite;

If the log-log graph paper is drawn so that the ordinate and abscissa scales are the same, i.e. the distance MEASURED with a ruler for a 10-fold CHANGE in the y variable is the same as for a 10-fold change in the x variable, A is the actual slope of the line. A is obtained by TAKING two points (X1, Y1) and (X2, Y2) on the line, and measuring the distances between Y2 and Y1 and X2 and X1 with a ruler:

12.

On which plot the Best fit-line is represented?(a) Histogram plot(b) Scatter plot(c) Probability plot(d) Dot plotThis question was posed to me during an interview.The origin of the question is Graph Paper with Logarithmic Coordinates in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct option is (b) Scatter plot

For explanation I would say: A line of BEST fit (or “trend” line) is a straight line that best represents the data on a scatter plot. This line may pass through some of the POINTS, NONE of the points, or all of the points.

13.

You are given a cube of pure copper. You measure the sides of the cube to find the volume and weigh it to find its mass. When you calculate the density using your measurements, you get 8.78 grams/cm^3. Coppers accepted density is 8.96 g/cm^3. What is your percent error?(a) 1%(b) 0.02%(c) 0.01%(d) 2%The question was asked in a job interview.This interesting question is from Errors in Data and Calculations in division Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct option is (d) 2%

Easy explanation: Experimental value = 8.78 g/cm^3

Accepted value = 8.96 g/cm^3

STEP 1: Subtract the accepted value from the experimental value.

8.78 g/cm^3 – 8.96 g/cm^3 = -0.18 g/cm^3

Step 2: Take the absolute value of step 1

|-0.18 g/cm^3| = 0.18 g/cm^3

Step 3: Divide that answer by the accepted value, 0.18g/cm^3 ÷ 8.96g/cm^3 = 0.02

Step 4: Multiply that answer by 100 and ADD the % symbol to express the answer as a percentage.

0.02 x 100 = 2

The percent error of your DENSITY CALCULATION was 2%.

14.

Which type of graph plot is used to analyze the effect of temperature on the rates of chemical reactions?(a) Dot plot(b) Normal probability plot(c) Arrhenius plot(d) Line weaver-Burk plotI got this question during an interview for a job.My doubt is from Data Analysis topic in portion Presentation and Analysis of Data of Bioprocess Engineering

Answer» CORRECT option is (c) Arrhenius plot

Explanation: An Arrhenius plot displays the logarithm of KINETIC constants (ln (K), ordinate axis) plotted against INVERSE temperature (1/T, abscissa). The Arrhenius equation can be written as:

ln (k) = ln (A) – (Ea/R) (1/T)

Where :

k = Rate constant

A = Pre-exponential factor

Ea = Activation energy

R = GAS constant

T = Absolute temperature, K

In the right, which represents the y-coordinate axis.
15.

Round off the number 425.68 to Tenths (1 decimal place), Hundredths (2 decimal place) and to Tens place?(a) 425.6, 425.68, 426(b) 425.7, 425.68, 430(c) 425.5, 426.78, 425(d) 425.7, 4257, 430The question was asked by my college director while I was bunking the class.My question comes from Errors in Data and Calculations topic in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right choice is (B) 425.7, 425.68, 430

Explanation: In the Tenths (1 decimal place), we will take 68 as a digit after the decimal and ROUND off it to the multiple of ten as 70, In the hundredths (2 decimal place), we will take both 68 as a number afterthe decimal because we have to take 2 decimal places and 6,8 both NUMBERS are greater than 5, In the TENS place, we will round the number before the decimal place to the closest multiple of ten, i.e. 430.

16.

Estimate the correct straight line in the log-linear scale?(a) (If the population is growing exponentially, the curve will be concave up), (If the growth is slower than exponential, you will see a straight line), (If the growth is faster than exponential, the curve will be concave down)(b) (If the population is growing exponentially, the curve will be concave down), (If the growth is slower than exponential, you will see a straight line), (If the growth is faster than exponential, the curve will be concave up)(c) (If the population is growing exponentially, you will see a straight line), (If the growth is slower than exponential, the curve will be concave down), (If the growth is faster than exponential, the curve will be concave up)(d) (If the population is growing exponentially, you will see a straight line), (If the growth is slower than exponential, the curve will be concave up), (If the growth is faster than exponential, the curve will be concave down)This question was addressed to me in examination.My question comes from Graph Paper with Logarithmic Coordinates topic in chapter Presentation and Analysis of Data of Bioprocess Engineering

Answer»

The correct choice is (c) (If the POPULATION is growing exponentially, you will SEE a STRAIGHT line), (If the growth is slower than exponential, the curve will be concave down), (If the growth is faster than exponential, the curve will be concave up)

Easy explanation: Functions plotted on the linear scale:

Figure 1:

Figure 2:

Functions plotted on the log-linear graph:

Figure 3 :

Figure 4 :

From the above graphs mentioned, Figures 2 and 4 GROWS exponentially while the function in Figures 1 and 3 grows slower than exponentially since the curve is concave down.

17.

Refer to the below graph, determine the specific growth rate µ and the doubling time.(a) (0.118 hours, 5.9 hours)(b) (0.119 hours, 5.9 hours)(c) (0.151 hours, 5.9 hours)(d) (0.119 hours, 5.9 hours)The question was asked in an online quiz.My enquiry is from Graph Paper with Logarithmic Coordinates in division Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct OPTION is (a) (0.118 hours, 5.9 hours)

To explain: The regression results show that μ = 0.118 hr^-1. The DOUBLING time,

TD = ln(2)/μ

= 0.693/0.118

= 5.9 hrs.

18.

If a student measured the mass of an object on a balance three times and obtained the values 14.568 g, 14.566 g, and 14.565 g, the range and percent of the average value between the measurements would be ____________(a) 0.005gm, 0.01%(b) 0.004gm,0.02%(c) 0.003gm,0.02%(d) 0.001gm,0.01%The question was asked during an interview.My question is taken from Errors in Data and Calculations topic in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

The correct option is (c) 0.003gm,0.02%

EXPLANATION: RANGE = (14.568 – 14.565) g = 0.003 g,

This is a reasonable range; the uncertainty appears in the last significant FIGURE of the measured value, as it should.

The average value for the mass of the object is 14.566 g. Therefore:

% of average = (0.003 g/14.566 g) x 100% = 0.02%

the range as a percent of average (RPA) has only one significant figure in this case because the numerator of the fraction has only one.

19.

If the substrate is given in molar concentration how would you define it’s Molarity and Molality based on the temperature condition?(a) Molarity and Molality both will change on effect of temperature(b) Molarity will change but not Molality(c) Molality will change but not Molarity(d) Normality of the reaction will changeThis question was addressed to me in homework.This interesting question is from Data Analysis in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right CHOICE is (b) Molarity will CHANGE but not Molality

The explanation: Molarity is the number of moles of solute per kilogram of the Solvent. That is because molarity does not depend on temperature, (neither on number of moles of solute nor MASS of solvent will be AFFECTED by changes of temperature). While molarity changes as temperature changes because molarity is affected by temperature. This is because it is BASED on the volume of the solution, and the volume of a substance will be affected by changes in temperature.

20.

A piece of iron rod was measured and found to be 120cms. But the actual value of the wood is 123cms. Find the relative error?(a) 3%(b) 2.5%(c) 2.43%(d) 3.43%This question was posed to me at a job interview.My doubt is from Errors in Data and Calculations topic in portion Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right answer is (c) 2.43%

To explain: Given:

True value = 123cms

Measured value = 120cms

Firstly, let us find out the ABSOLUTE ERROR.

We know by subtracting true value with measured value we GET absolute error = 123 – 120 = 3cms

THEREFORE, absolute error = 3cms

Now, let us find out the relative error = absolute error/True value x 100

= 3/123 x 100 = 2.43%

Therefore, the relative error for the given values = 2.43%.

21.

What is the basic Optical Density (O.D) of the bacterial cells?(a) 680nm(b) 600nm(c) 200nm(d) 280nmI had been asked this question by my school principal while I was bunking the class.The query is from Data Analysis topic in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right option is (B) 600NM

The explanation: 600nm is a WAVELENGTH of light that is minimally absorbed by material within cells. Therefore, they can be more reliably used than other wavelengths (in most common situations). To SIMPLY, measure the scattering of light due to turbidity (just general presence of PARTICLES in a solution).

22.

On which type of plot the best fit line is represented?(a) Dot plot(b) Line weaver- Burk plot(c) Scatter plot(d) Bland- Altman plotThe question was posed to me in quiz.My question is taken from Data Analysis topic in division Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct answer is (c) Scatter plot

The EXPLANATION: A line of best FIT is a STRAIGHT line drawn through the center of a group of data points plotted on a scatter plot. Scatter plots depict the results of gathering data on two variables. The line of best fit shows whether these two variables appear to be correlated and can be used to help identify trends occurring within the dataset. ANALYSTS may use the line of best fit when determining a relationship between two variables where one variable is INDEPENDENT and one variable is being examined for dependency.

23.

124g cells/g glucose, (ii) 0.897g alcohol/g glucose(a) (i) 0.127g cells/g glucose, (ii) 0.694g alcohol/g glucose(b) (i) 0.145g cells/g glucose, (ii) 0.890g alcohol/g glucose(c) (i) 0.126g cells/g glucose, (ii) 0.693g alcohol/g glucoseThis question was posed to me in an interview for internship.I'd like to ask this question from Graph Paper with Logarithmic Coordinates in portion Presentation and Analysis of Data of Bioprocess Engineering

Answer»
24.

The diauxic growth curve mainly found in E.coli in the case of two types of nutrients is found in Batch culture is based on which equation?(a) Monod equation(b) Michaelis – Menten equation(c) Schrodinger equation(d) Lyapunov equationThis question was posed to me during an interview.This intriguing question originated from Data Analysis in chapter Presentation and Analysis of Data of Bioprocess Engineering

Answer»

The correct option is (a) Monod equation

The explanation: Jacques Monod discovered diauxic growth in 1941 during his EXPERIMENTS with Escherichia coli and BACILLUS subtilis. While growing these bacteria on various combinations of sugars during his doctoral thesis RESEARCH, Monod OBSERVED that often two distinct growth phases are CLEARLY visible in batch culture.

25.

Which of the following is correct to bacterial growth curve?(a) Representation of the asexual reproduction of the bacteria(b) Representation of the sexual reproduction of the bacteria(c) Representation between sexual and asexual reproduction of bacteria(d) Representation of the mutation of the bacteriaThis question was addressed to me in semester exam.The question is from Graph Paper with Logarithmic Coordinates in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right choice is (a) Representation of the asexual reproduction of the bacteria

The explanation: The representation is of the Bacterial growth which is the asexual reproduction, or cell division, of a bacterium into two daughter CELLS, in a process called binary FISSION. PROVIDING no mutational event occurs, the RESULTING daughter cells are GENETICALLY identical to the original cell.

26.

The accepted value for the density of gold metal is 19.31 g/cm^3. If a student measured the mass and volume of a sample of gold, and obtained a value of 19.03 g/cm^3, the percent difference would be ______(a) -1.5%(b) 1.5%(c) -0.5%(d) 0.5%The question was asked in quiz.This question is from Errors in Data and Calculations topic in chapter Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct answer is (a) -1.5%

To ELABORATE: % DIFFERENCE = [(19.03 – 19.31) G/19.31 g] x 100% = – 1.5%

A percent difference can be POSITIVE or negative; the sign shows whether the experimental value is HIGHER or lower than the actual or theoretical value. This distinction is not used in precision since all values are experimental.

27.

Essentially in an experiment involving bacteria, we measure Optical density of the media where the bacteria grow at different time point from the same flask. If we have 2 or more types of bacteria and want to investigate whether the growth curve of those bacteria are significantly different, what do you think the best analysis can be applied for this purpose?(a) Linear regression analysis or general regression analysis(b) Non- linear regression analysis or general regression analysis(c) Logistic regression analysis(d) Stepwise regression analysisThe question was asked in an internship interview.Question is from Data Analysis topic in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right option is (b) Non- LINEAR regression analysis or general regression analysis

The explanation is: If the growth rates fit a POLYNOMIAL CURVE, you can use General Regression and enter the bacteria type as categorical variable. Otherwise, you may need to use NONLINEAR regression SEPARATELY for each type.

28.

For what MANOVA is used for?(a) Detection of biological samples(b) Detection of the best fit curve(c) Comparison between monovariate samples(d) Comparison between multivariate samplesThis question was posed to me in exam.My doubt stems from Data Analysis in chapter Presentation and Analysis of Data of Bioprocess Engineering

Answer»

The correct option is (d) Comparison between multivariate samples

To explain I would say: In statistics, multivariate analysis of variance (MANOVA) is a procedure for COMPARING multivariate SAMPLE means. As a multivariate procedure, it is USED when there are TWO or more dependent variables and is typically followed by significance tests involving INDIVIDUAL dependent variables separately.

29.

In below mentioned options which one defines correctly the semi-log graph?(a) 10-cycle semi-logarithmic paper(b) 6- cycle semi-logarithmic paper(c) 2- cycle semi-logarithmic paper(d) 1- cycle semi logarithmic paperI had been asked this question in a national level competition.The doubt is from Graph Paper with Logarithmic Coordinates topic in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Correct option is (d) 1- cycle semi LOGARITHMIC paper

For EXPLANATION I would say: In one-cycle semi-logarithmic paper, the vertical SCALE goes from 1 to 10 and within the One order of magnitude (10^1) whereas, in others the magnitude varies like in 2 – cycle there is the magnitude of two i.e. (10^2) and likewise other options have different magnitudes according to their number of cycles.

30.

Which of the following is correct according to calculating relative error as a measure of Precision?(a) (Absolute error/ True value)*100(b) (Absolute error/True value)(c) (measurement being taken/Absolute error)*100(d) (Absolute error/measurement being taken)The question was asked during an online interview.I want to ask this question from Errors in Data and Calculations topic in section Presentation and Analysis of Data of Bioprocess Engineering

Answer» CORRECT option is (d) (Absolute error/measurement being taken)

Best EXPLANATION: Relative error (RE) — when USED as a measure of precision — is the RATIO of the absolute error of a measurement to the measurement being taken. In other words, this TYPE of error is relative to the size of the item being measured. RE is expressed as a percentage and has no units.