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What is the basic Optical Density (O.D) of the bacterial cells?(a) 680nm(b) 600nm(c) 200nm(d) 280nmI had been asked this question by my school principal while I was bunking the class.The query is from Data Analysis topic in section Presentation and Analysis of Data of Bioprocess Engineering

Answer»

Right option is (B) 600NM

The explanation: 600nm is a WAVELENGTH of light that is minimally absorbed by material within cells. Therefore, they can be more reliably used than other wavelengths (in most common situations). To SIMPLY, measure the scattering of light due to turbidity (just general presence of PARTICLES in a solution).



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