1116 + Interview Questions in BIOTECHNOLOGY IN GENERAL AWARENESS Page 5 InterviewSolution

201.	The DNA molecules of which the gene of interset is integrated for cloning is calledA. CarrierB. TransformerC. VectorD. Template
Answer» Correct Answer - C

Discussion

202.	Which one of the following processes results in the formation of clone of bacteria ?A. Binary fissionB. ConjugationC. TransformationD. Transduction
Answer» Correct Answer - A

Discussion

203.	The DNA molecules of which the gene of interset is integrated for cloning is calledA. transformerB. vectorC. templateD. carrier
Answer» Correct Answer - b

Discussion

204.	Cloning gene is process where:A. gene is cloned in an animalB. fragments of DNA are transferred from one organism to another usually carried on a DNA vectorC. fragment of DNA cloned in the same organism using carrierD. DNA is cloned in plants
Answer» Correct Answer - B

Discussion

205.	Which is used in gene cloning ?A. LomasomesB. MesosomesC. PlasmidD. Nucleotides
Answer» Correct Answer - C Plasmids are used in gene cloning

Discussion

206.	Having become an expert on gel electrophoresis you are asked to examine a gel for a colleague .Where do you find the smallest segments of DNA?
Answer» The smallest segment of DNA are found near the positive electrode farthest away from the wells.

Discussion

207.	Becoming an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would you find the smallest segments of DNA ?A. Near the positive electrode, farthest away from the wellsB. Near the negative electode, close to the wellsC. Near the top, near the negative poleD. Near the middle, they tend to slow down after the first few minutes
Answer» Correct Answer - a

Discussion

208.	Gel electrophoresis is used forA. Construction of recombinant DNA by joining with cloning vectorsB. Isolation of DNA moleculesC. Cutting of DNA into fragmentsD. Separation of DNA fragment according to their size
Answer» Correct Answer - D Gel electrophoresis is used for separation of DNA fragments according to their size

Discussion

209.	Bio pesticide includeA. Only bioinsecticideB. Only bioherbicideC. Bioinsecticide & bioherbicideD. Bioherbicide, bioinsecticide & biofertilisers
Answer» Correct Answer - C

Discussion

210.	Which biocontrol agent in very common in root ecosystem & is effective against several plant pathogens.A. BaculovirusesB. TrichodermaC. Nucleopolyhedro virusD. Ladybird beetle & Dragonflies
Answer» Correct Answer - B

Discussion

211.	Bioweapons are :A. invisibleB. low costC. difficult to detectD. all of these
Answer» Correct Answer - D

Discussion

212.	Which one of the following is used as vector for cloning into higher organisms ?A. Salmonella typhimuriumB. Rhizopus nigricansC. RetrovirusD. Baculovirus
Answer» Correct Answer - C Retrovirus is used as vector for cloning into higher organisms (animals)

Discussion

213.	Which one of the following is used as vector for cloning genes into higher organisms ?A. BaculovirusB. Salmonella typhimuriumC. Rhizopus migricansD. Retrovirus
Answer» Correct Answer - a

Discussion

214.	Vector for T-DNA isA. Thermus aquaticusB. Salmonella typhimuriumC. Agrobacterium tumefaciensD. Escherichia coli
Answer» Correct Answer - c

Discussion

215.	Choose the correcte statement with reference to 'Dolly'A. She was created by taking nucleus from unfertilized eggs and cytoplasm from unfertilized eggs.B. She was created by taking cytoplams from udder cell and nucleus from unfertilized egge.C. She was created by taking nucleus from udder cells and cytoplasm from unfertilized eggs.D. She was created by taking cytoplasm from udder cell and nucleus from fertilized egg.
Answer» Correct Answer - C

Discussion

216.	Restriction enzymes are also calledA. Molecular markersB. VectorsC. CarriersD. Molecular scissors
Answer» Correct Answer - D Restriction enzymes are called molecular scissors

Discussion

217.	In gel electrophoresis, differential mobility of DNA depends uponA. Helical natural of DNAB. Double stranded natural of DNAC. Charge and size of DNAD. Hydrogen bonding between bases
Answer» Correct Answer - C In gel electrophoresis, differential mobility of DNA depends upon charge and size of DNA

Discussion

218.	Natural genetic engineer is:A. Bacillus subtilliB. Escherichia coliC. Pseudomonas sppD. Agrobacterium tumefaciens
Answer» Correct Answer - D

Discussion

219.	It is sometimes necessary to genetically engineer mammalian cells to produce protein because they:A. are easier to grow than bacteriaB. can read eukaryotic genes, and bacteria cannotC. can produce larger quantities of proteins than bacteriaD. can add sugars to make glycoproteins and bacteria cannot
Answer» Correct Answer - D

Discussion

220.	Genetic engineering is employed to produce vaccines for:A. Herpes virusB. Hepatitis BC. Both of theseD. None of these
Answer» Correct Answer - C

Discussion

221.	Polymerase chain reaction employA. Primers and DNA ligaseB. DNA ligase onlyC. DNA polymerase onlyD. Primers and DNA polymerase
Answer» Correct Answer - D Polymerase chain reaction employs primers and DNA polymerase

Discussion

222.	Polymerase chain reaction is most useful inA. RNA synthesisB. DNA amplificationC. Protein synthesisD. Amino acid synthesis
Answer» Correct Answer - B Polymerase chain reaction is useful in DNA amplification

Discussion

223.	Bioreactors are useful inA. separation and purification of a productB. processing of large volumes of cultureC. microinjectionD. isolation of genetic material
Answer» Correct Answer - b Bioreactors are the large volume vessels in which raw materials are biologically converted into specific products, individual enzymes, etc. uisng microbial, plant, animal or human cells. It provides the optimal conditions for achieving the desired product by providing optimum growth conditions like pH, temperature, substrate, salt, vitamins and oxygen.

Discussion

224.	Bioreactors are useful inA. Separation and purification of a productB. MicroinjectionC. Processing of large volume of cultureD. Isolation of genetic material
Answer» Correct Answer - C Bioreactors are useful in processing of large volume of culture (`100 -1000` litres)

Discussion

225.	A typical bioreactor has a. An agitator system b. An oxygen control delivery system c. A foan control system d. A temperature control system e. A pH control system f. Sampling portsA. a, b and cB. a, b, c and dC. a, b, b, d and cD. a, b, c,d,e and f
Answer» Correct Answer - D A typical bioreactor has a. An agitator system b. An oxygen delivery system c. A foam control system d. A temperature control system e. A pH control system f. Sampling ports

Discussion

226.	Bioreactor is a vessel/device in whichA. Chemical process involving microorganisms is carried outB. Chemical process involving radioactive substance is carried outC. Potentially hazardous microbes are handledD. Electrochemical processes are carried out
Answer» Correct Answer - A Boreaction is a vessel/device in which chemical process involving microorganisms is carried out

Discussion

227.	Recombinant DNA technology revolution actually began with the discovery ofA. PlasmidsB. Restriction endonculeasesC. Complementary DNAD. PCR
Answer» Correct Answer - B Recombinant -DNA technology revolution actually began with the discovery of restriction endonculeases

Discussion

228.	Execute the engineered biosynthetic pathway which leads to the production of valuable secondary metabolites for their over-production.
Answer» Biosynthetic pathway which leads to the production of secondary metabolites are being engineered for their over-production to reduce the cost. The over expression of the gene which encodes for the first enzyme in the pathway which results in higher levels of desired end products.

228.

Execute the engineered biosynthetic pathway which leads to the production of valuable secondary metabolites for their over-production.

Answer»

Biosynthetic pathway which leads to the production of secondary metabolites are being engineered for their over-production to reduce the cost.

The over expression of the gene which encodes for the first enzyme in the pathway which results in higher levels of desired end products.

Discussion

229.	State any two advantages of using Pichia pastoris as a eukaryotic expression host.
Answer» Advantages of Pichia pastoris as a eukaryotic expression host: (i) Has strong inducible promoters (ii) Is capable of making post-translational modifications (iii) Downstream processing is simpler as Pichia does not secrete its own proteins into the fermentation medium

229.

State any two advantages of using Pichia pastoris as a eukaryotic expression host.

Answer»

Advantages of Pichia pastoris as a eukaryotic expression host:

(i) Has strong inducible promoters

(ii) Is capable of making post-translational modifications

(iii) Downstream processing is simpler as Pichia does not secrete its own proteins into the fermentation medium

Discussion

230.	Select the correct statement(s)A. Agarose gel electrophoresis is used to check the progression of a restriction enzyme digestionB. DNA is negative charged and lipophilic in nature,C. Agrobacterium and Retrovirus both transforms normal animal cells into cancerous cellsD. Both A and C
Answer» Correct Answer - A `` DNA is negatively charged and hydrophilic in nature `` Agrobacterium and Retrovirus both transforms normal plant animal cells respectively into cancerous cells.

Discussion

231.	A linear DNA fragment and a plasmid has three restriction sites for EcoRI how many fragments will be produced from linear DNA and plasmid respectively.
Answer» Number of fragments of linear DNA = 4 Number of fragments of plasmid = 3

231.

A linear DNA fragment and a plasmid has three restriction sites for EcoRI how many fragments will be produced from linear DNA and plasmid respectively.

Answer»

Number of fragments of linear DNA = 4

Number of fragments of plasmid = 3

Discussion

232.	Which of the following is used a biological warfare agent?A. Smallpox virusB. Bacillus anthracisC. Both of theseD. None of these
Answer» Correct Answer - C

Discussion

233.	Which of the following is associated with genetic engineering ?A. PlastidsB. PlasmidsC. ATPaseD. Histones
Answer» Correct Answer - b

Discussion

234.	Term which can be used as synonym of genetic engineering in many cases in known asA. gene manipulationB. gene therapyC. Both (a) and (b)D. recombinant DNA technology
Answer» Correct Answer - d

Discussion

235.	Main phenomenon on which genetic engineering is based isA. alteration of genetic constitution of cellsB. insertion of plasmid in cellsC. alteration in cell organellesD. joining of two cells
Answer» Correct Answer - a

Discussion

236.	Which type of DNA library would you prefer for liver cells. Give proper explanation for making such a library.
Answer» c DNA library would be preferred. mRNA molecules are highly unstable as they are easily degraded by RNAses .Therefore mRNA molecules are copied into the more stable DNA (now called cDNA) before cloning. The construction of a cDNA library begins with the isolation of mRNA from a given cell type or tissue which are copied into cDNA using a special enzyme called reverse transcriptase. The procedure results in double-stranded cDNA which can be incorporated into vectors such as pBR322.These recombinant vectors are transformed into host bacterial cells eg. E.coli. This forms a cDNA library.

236.

Which type of DNA library would you prefer for liver cells. Give proper explanation for making such a library.

Answer»

c DNA library would be preferred.

mRNA molecules are highly unstable as they are easily degraded by RNAses .Therefore mRNA molecules are copied into the more stable DNA (now called cDNA) before cloning. The construction of a cDNA library begins with the isolation of mRNA from a given cell type or tissue which are copied into cDNA using a special enzyme called reverse transcriptase. The procedure results in double-stranded cDNA which can be incorporated into vectors such as pBR322.These recombinant vectors are transformed into host bacterial cells eg. E.coli. This forms a cDNA library.

Discussion

237.	Insect pest resistant Bt-cotton plant was developed using:A. micropropagationB. somaclonal variationC. somatic hybridizationD. transgenic technology
Answer» Correct Answer - D

Discussion

238.	In case of Bt Cotton how does the toxic insecticide protein produced by bacterium kill the insect pest but not the cells of Bacillus thuringiensis where the toxic protein is generated ?
Answer» The toxin produced by Bacillus thuringiensis is an endotoxin called cry protein. It is crystalline and non toxic when formed being in protoxin stage. As it reaches the gut of insects, the cry protein is converted into toxic ‘ and soluble state. It attaches the receptors present on the epithelial cells of the gut produces pores and kills the cells resulting in death of the insects.

Discussion

239.	Which of the following option is correct for recombinant DNA technologyA. endonuclease enzyme removes nucleotides from the ends of DNAB. Exonuclease enzyme removes nucleotides from site within DNAC. Endonuclease enzyme cut long polantric DNA strandD. Exonuclease enzyme removes nucleotides from ends of DNA
Answer» Correct Answer - d The most correct and appropriate option is that exonuclease removes nucleotides from the teminal ends of DNA and cut a short palindromic DNA strand. Endonuclease remove nucleotides from positions within the DNA.

Discussion

240.	The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because ofA. Inactivation of glycosidase enzyme in recombinant bacteriaB. Non-recombinant bacteria containing betaglactosidaseC. Insertional inactivation of alpha-galactosidase in non-recombinant bacteriaD. Insertional inactivation of alpha-galactosidase in recombinant bacteria
Answer» Correct Answer - D Because of insertional inactivation of alpha-galactosidase in recombinant bacteria.

Discussion

241.	The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because ofA. insertional inactivation of `alpha`-galactosidase in non-recombinant bacteriaB. insertional inactivation of `beta`-galactosidase in recombinant bacteriaC. inactivation of glycosidase enzyme in bacteriaD. non-recombinant bacteria containing `beta`-galactosidase
Answer» Correct Answer - b The colonies of recombinant of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria due to inactivation of beta-galactosidase enzyme in recombinant bacteria.

Discussion

242.	The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria be cause beA. Insertional inactivation of alpha galactosidase in recombinant bacteriaB. Inactivation of glycosidase enzyme in recombinant bacteriaC. Non-recombinant bacteria containing beta galactosidaseD. Insertional inactivation of alpha galactosidase in non-recombinant bacteria
Answer» Correct Answer - C The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of non-recombinant bacteria containing beta galactosidase

Discussion

243.	The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because ofA. Inactivation of glycosidase enzyme in recombinant bacteriaB. Non-recombinant bacteria containing beta-galactosidaseC. Insertional inactivation of alpha-galactosidase in non-recombinant bacteriaD. Insertional inactivation of beta-galactosidase in recombinant bacteria
Answer» Correct Answer - D

Discussion

244.	Stirred-tank reactor is usually cylindrical or with a curved base. Why?A. Mixing of the reactor contentsB. Control the temperautre of the reactorC. Control the pH of the reactorD. All of the above
Answer» Correct Answer - A `` A stirred-tank bioreactor is usually cylinderical or with a curved base to facilitate the mixing of the reactor contents `` The stirrer faciliates even mixing and `O_(2)`-availability throughout the bioreactor. Alternatively air can be bubbled through the reactor.

Discussion

245.	Select the incorrect statements(s)A. A foreign DNA can be ligated at the BamHI site of ampicillin resistance gene in the vector pBR 322B. Some plasmids may have only one or 2 copies per cell wheras others may have 15-100 copies per cell.C. In almost all recombinant technologies, the ultimate aim is to produce a desirable proteinsD. All of the above
Answer» Correct Answer - A A foreign DNA can be ligated at the BamH-I site of tetracycline resistance gene in the vector pBR 322

Discussion

246.	Choose the incorrect match.A. Western blotting - ProteinB. Northern blotting - RNAC. Southern blotting - DNAD. DNA fingerprinting - Protein
Answer» Correct Answer - d

Discussion

247.	eDNA stands forA. copy DNAB. coupled DNAC. complementary DNAD. compound DNA
Answer» Correct Answer - c

Discussion

248.	By which method rDNA is directly injected into the nucleus of animal cell ?
Answer» Correct Answer - Microinjection

Discussion

249.	“The prophase I of meiosis plays a vital role in rDNA formation” Justify the statement.
Answer» This is because crossing over occurs in this phase which helps to produce recombinants.

Discussion

250.	A technique used in making copies of a specific segment of DNA involvesi. ligase chain reactionii. transcriptioniii. polymerase chain reactioniv. translation.
Answer» (iii) polymerase chain reaction.

Discussion

Explore topic-wise InterviewSolutions in .

The DNA molecules of which the gene of interset is integrated for cloning is calledA. CarrierB. TransformerC. VectorD. Template

Which one of the following processes results in the formation of clone of bacteria ?A. Binary fissionB. ConjugationC. TransformationD. Transduction

The DNA molecules of which the gene of interset is integrated for cloning is calledA. transformerB. vectorC. templateD. carrier

Cloning gene is process where:A. gene is cloned in an animalB. fragments of DNA are transferred from one organism to another usually carried on a DNA vectorC. fragment of DNA cloned in the same organism using carrierD. DNA is cloned in plants

Which is used in gene cloning ?A. LomasomesB. MesosomesC. PlasmidD. Nucleotides

Having become an expert on gel electrophoresis you are asked to examine a gel for a colleague .Where do you find the smallest segments of DNA?

Gel electrophoresis is used forA. Construction of recombinant DNA by joining with cloning vectorsB. Isolation of DNA moleculesC. Cutting of DNA into fragmentsD. Separation of DNA fragment according to their size

Bio pesticide includeA. Only bioinsecticideB. Only bioherbicideC. Bioinsecticide & bioherbicideD. Bioherbicide, bioinsecticide & biofertilisers

Which biocontrol agent in very common in root ecosystem & is effective against several plant pathogens.A. BaculovirusesB. TrichodermaC. Nucleopolyhedro virusD. Ladybird beetle & Dragonflies

Bioweapons are :A. invisibleB. low costC. difficult to detectD. all of these

Which one of the following is used as vector for cloning into higher organisms ?A. Salmonella typhimuriumB. Rhizopus nigricansC. RetrovirusD. Baculovirus

Which one of the following is used as vector for cloning genes into higher organisms ?A. BaculovirusB. Salmonella typhimuriumC. Rhizopus migricansD. Retrovirus

Vector for T-DNA isA. Thermus aquaticusB. Salmonella typhimuriumC. Agrobacterium tumefaciensD. Escherichia coli

Restriction enzymes are also calledA. Molecular markersB. VectorsC. CarriersD. Molecular scissors

In gel electrophoresis, differential mobility of DNA depends uponA. Helical natural of DNAB. Double stranded natural of DNAC. Charge and size of DNAD. Hydrogen bonding between bases

Natural genetic engineer is:A. Bacillus subtilliB. Escherichia coliC. Pseudomonas sppD. Agrobacterium tumefaciens

It is sometimes necessary to genetically engineer mammalian cells to produce protein because they:A. are easier to grow than bacteriaB. can read eukaryotic genes, and bacteria cannotC. can produce larger quantities of proteins than bacteriaD. can add sugars to make glycoproteins and bacteria cannot

Genetic engineering is employed to produce vaccines for:A. Herpes virusB. Hepatitis BC. Both of theseD. None of these

Polymerase chain reaction employA. Primers and DNA ligaseB. DNA ligase onlyC. DNA polymerase onlyD. Primers and DNA polymerase

Polymerase chain reaction is most useful inA. RNA synthesisB. DNA amplificationC. Protein synthesisD. Amino acid synthesis

Bioreactors are useful inA. separation and purification of a productB. processing of large volumes of cultureC. microinjectionD. isolation of genetic material

Bioreactors are useful inA. Separation and purification of a productB. MicroinjectionC. Processing of large volume of cultureD. Isolation of genetic material

A typical bioreactor has a. An agitator system b. An oxygen control delivery system c. A foan control system d. A temperature control system e. A pH control system f. Sampling portsA. a, b and cB. a, b, c and dC. a, b, b, d and cD. a, b, c,d,e and f

Bioreactor is a vessel/device in whichA. Chemical process involving microorganisms is carried outB. Chemical process involving radioactive substance is carried outC. Potentially hazardous microbes are handledD. Electrochemical processes are carried out

Recombinant DNA technology revolution actually began with the discovery ofA. PlasmidsB. Restriction endonculeasesC. Complementary DNAD. PCR

Execute the engineered biosynthetic pathway which leads to the production of valuable secondary metabolites for their over-production.

State any two advantages of using Pichia pastoris as a eukaryotic expression host.

Select the correct statement(s)A. Agarose gel electrophoresis is used to check the progression of a restriction enzyme digestionB. DNA is negative charged and lipophilic in nature,C. Agrobacterium and Retrovirus both transforms normal animal cells into cancerous cellsD. Both A and C

A linear DNA fragment and a plasmid has three restriction sites for EcoRI how many fragments will be produced from linear DNA and plasmid respectively.

Which of the following is used a biological warfare agent?A. Smallpox virusB. Bacillus anthracisC. Both of theseD. None of these

Which of the following is associated with genetic engineering ?A. PlastidsB. PlasmidsC. ATPaseD. Histones

Term which can be used as synonym of genetic engineering in many cases in known asA. gene manipulationB. gene therapyC. Both (a) and (b)D. recombinant DNA technology

Main phenomenon on which genetic engineering is based isA. alteration of genetic constitution of cellsB. insertion of plasmid in cellsC. alteration in cell organellesD. joining of two cells

Which type of DNA library would you prefer for liver cells. Give proper explanation for making such a library.

Insect pest resistant Bt-cotton plant was developed using:A. micropropagationB. somaclonal variationC. somatic hybridizationD. transgenic technology

In case of Bt Cotton how does the toxic insecticide protein produced by bacterium kill the insect pest but not the cells of Bacillus thuringiensis where the toxic protein is generated ?

Stirred-tank reactor is usually cylindrical or with a curved base. Why?A. Mixing of the reactor contentsB. Control the temperautre of the reactorC. Control the pH of the reactorD. All of the above

Choose the incorrect match.A. Western blotting - ProteinB. Northern blotting - RNAC. Southern blotting - DNAD. DNA fingerprinting - Protein

eDNA stands forA. copy DNAB. coupled DNAC. complementary DNAD. compound DNA

By which method rDNA is directly injected into the nucleus of animal cell ?

“The prophase I of meiosis plays a vital role in rDNA formation” Justify the statement.

A technique used in making copies of a specific segment of DNA involvesi. ligase chain reactionii. transcriptioniii. polymerase chain reactioniv. translation.