InterviewSolution
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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which one of the following techniques made it possible to gnetically engineer living organisms ?A. Heavier isotope labellingB. HybridizationC. Recombinant DNA techniquesD. X-ray difraction |
| Answer» Correct Answer - C | |
| 2. |
Which of the following bonds are formed by action of DNA ligase ?A. Sugar-phosphate bondB. Phosphodiester bondC. Phosphate-phosphate bondD. Both (1) & (2) |
| Answer» Correct Answer - D | |
| 3. |
Who is given for constructing first atrificial recombinant molecule ?A. Hargovind KhoranaB. Staniey Cohen and Herbert BoyerC. Linus paulingD. Arber and Nathans |
| Answer» Correct Answer - B | |
| 4. |
In recombinant DNA technology, a plasmid vector is cleaved byA. Modified DNA ligaseB. A heated alkaline solutionC. The same enzyme that cleaves donor DNAD. Different enzyme that cleaved the donor DNA |
| Answer» Correct Answer - C | |
| 5. |
Who among the following was awarded the Nobal Prize for the development of PCR technique ?A. Herbert BoyerB. Hargovind KhuraneC. Kary MullisD. Athur Kornberg |
| Answer» Correct Answer - C | |
| 6. |
Who among the following was awarded the Nobal Prize for the development of PCR technique ?A. Herbert BoyerB. Kary MullisC. Rene DescratesD. Andrew Fire |
| Answer» Correct Answer - B | |
| 7. |
Which is used in gene cloningA. NucleoidsB. LomasomesC. MesosomesD. Plasmids |
| Answer» Correct Answer - D | |
| 8. |
pBR322 was the first attificila cloning vector to be constructed. What does "BR" stands for ?A. Bacteriophage and RecombiantB. Boliver an RodriguezC. Boyer and ReplicativeD. None of these |
| Answer» Correct Answer - B | |
| 9. |
The DNA molecule to which the gene of interset is integrated for cloning is calledA. CarrierB. TransformerC. VectorD. Template |
| Answer» Correct Answer - C | |
| 10. |
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because ofA. Insertional inactivation of alpha - galactosidase in non-recombinant bacteriaB. Insertional inactivation of alpha - galactosidase in recombinant bacteriaC. Inactivation of glycosidase enzyme in recombinant bacteriaD. Non-recombinant bacteria containing beta-galactosidase |
| Answer» Correct Answer - B | |
| 11. |
Which is responsible for controlling the copy number of the linked DNA in a plasmid ? |
| Answer» Origin of replication | |
| 12. |
Micro-injection is a method used toA. produce sticky ends of DNAB. provide protection against pathogenC. purify the DNAD. inject recombinant DNA into the nucleus of an animal cell. |
| Answer» Correct Answer - D | |
| 13. |
Match the terms given in column I with their definitions in column II and select the correct answer from codes given below. `{:(Column I,Column II),(A."Transformation",(i)"Sequences cut by restriction enzymes"),(B."Recognition site",(ii)"Process by which DNA fragments are separated based on their size"),(C."Gel electrophoresis",(iii)"Plasmid DNA that has incorporated human DNA"),(D."Recombinant DNA",(iv)"Process by which bacteria take up pieces of DNA from the environment"):}`A. `A-(iii),B-(i),C-(ii),D-(iv)`B. `A-(iv),B-(i),C-(ii),D-(iii)`C. `A-(i),B-(ii),C-(iii),D-(iv)`D. `A-(ii),B-(iii),C-(iv),D-(i)` |
| Answer» Correct Answer - B | |
| 14. |
The extraction of DNA fragment from agarose gel is called_______ .A. Down stream processingB. Upstream processingC. ElutionD. Insertional inactivation |
| Answer» Correct Answer - C | |
| 15. |
A: To extract RNA from tomato cells it must first be treated with cellulase, protease and then deoxyribonuclease. R: Deoxyribonuclease will digest DNA while RNA will be intact.A. If both Assertion & Reason are true and the reason is the correct explanation of the assertionB. If both Assertion & Reason are true but the reason is not the correct explanation of the assertionC. If Assertion is true statement but Reason is falseD. If both Assertion and Reason are false statements |
| Answer» Correct Answer - B | |
| 16. |
DNA cannot pass through a cell membrane asA. it is too big to cross the memebraneB. it is a hydrophilic moleculeC. membrane does not have specific proteins to facilitate the transportD. none of these |
| Answer» Correct Answer - B | |
| 17. |
The linking of antibiotic resistance gene with the plasmid vector became possible withA. ExonucleasesB. DNA ligaseC. EndouncleasesD. DNA polymerase |
| Answer» Correct Answer - B | |
| 18. |
Gel electrophoresis is used forA. Isolation of DNA moleculeB. Cutting of DNA into fragmentsC. Separation of DNA fragments according to their sizeD. Construction of recombinant DNA by joining with cloning vectors |
| Answer» Correct Answer - C | |
| 19. |
Seperation and purification by filtiration , centrifugation of desired compound produced in bioreactor is a part ofA. Downstream processing onlyB. Scaling up and downstream processingC. Upstream processingD. Screening for recombinants and downstream processing |
| Answer» Correct Answer - A | |
| 20. |
Name E.coli cells carry resistance against which of the following antibiotics ?A. ChloramphenicolB. AmpicillinC. TetracyclineD. None of these |
| Answer» Correct Answer - D | |
| 21. |
List two essential characteristics of vectors |
|
Answer» 1. It should help in easy linking of foreign DNA 2. It should enable easy selection of recombinants from non-recombinants |
|
| 22. |
Match column I with column II and select the correct answer from the given codes. `{:(Column I,Column II),(A.amp^(R) "gene",(i)"Artificial plasmid"),(B."Separation of DNA fragments",(ii)"Selectable marker"),(C."Hindlll",(iii)"Electrophoresis"),(D.pBR322,(iv)"Haemophilus influenzea"):}`A. `A-(iii),B-(ii),C-(i),D-(iv)`B. `A-(iv),B-(i),C-(iii),D-(ii)`C. `A-(iii),B-(iii),C-(iv),D-(i)`D. `A-(iii),B-(iv),C-(i),D-(iii)` |
| Answer» Correct Answer - C | |
| 23. |
Unique restriction sites are present in a small region of DNA in a vector . What is this region called / |
| Answer» MCS (multiple cloning site) | |
| 24. |
A researcher identifies a naturally occuring variant possessing characteristics of interest . This plant is selectively bred. This is an example ofA. Traditional plant breedingB. Transgenic technologyC. Mutant selectionD. Cross breeding |
| Answer» Correct Answer - A | |
| 25. |
What is the end product obtained after exonuclease activity ? |
| Answer» Correct Answer - Nucleotides | |
| 26. |
Name the enzyme used to combine two fragments of DNA . |
| Answer» Correct Answer - DNA ligase | |
| 27. |
The correct sequence of making a cell competent isA. treatment with divalent cation `rarr` incubation of cells with recombinant `DNA` of ice `rarr` heat shock `(42^(@)C)rarr` placing on iceB. heat shock `(42^(@)C)rarr` incubation of cells with recombinant DNA on ice `rarr` treatment with divalent cations `rarr` placing on iceC. treatment with divalent cation `rarr` placing on ice `rarr` incubation of cells with recombinant DNA on ice `rar` heat shock `(42^(@)C)`D. incubation of cells with recombinant DNA on ice `rarr` heat shock `(42^(@)C) rarr `treatment with divalent cations `rarr` placing on ice. |
| Answer» Correct Answer - A | |
| 28. |
Which of the following explains the role of the heat shock during transformation ?A. Binding of DNA to bacterial cell wallB. Uptake of DNA through transient poresC. Decreased efficiency of transformationD. All of these |
| Answer» Correct Answer - B | |
| 29. |
Which of these are most widely used in genetic engineering ?A. PlastidB. PlasmidC. MitrocondriaD. Ribosome |
| Answer» Correct Answer - B | |
| 30. |
Read the given statements and select the correct option Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome. Statement 2: Presence of insert results into insertional inactivation of `beta`-galactosidase enzyme and the colonies do not produce any colour.A. Both statements 1 and 2 are correctB. statement 1 is correct but statement 2 is incorrectC. statement 1 is incorrect but statement 2 is correctD. Both statements 1 and 2 are incorrect |
| Answer» Correct Answer - A | |
| 31. |
The tumor including capacity of _A_ is located in large extra-chromosomal plasmid called Ti plasmid. Choose the option which correctly fills up the blanks _A_A. Thermus aquaticusB. Salmonella typhimuriumC. E.coliD. Agrobacterium tumefaciens |
| Answer» Correct Answer - D | |
| 32. |
M 13 can be classified as which kind of vector ?A. CosmidB. YACC. BACD. Bacteriophage |
| Answer» Correct Answer - D | |
| 33. |
During the process of isolation of DNA, chilled ethanol is added toA. Remove proteins such as histonesB. Precipitate DNAC. Break open the cell to release DNAD. Facilitate action of restriction enzymes |
| Answer» Correct Answer - B | |
| 34. |
A tumor including plasmid widely used in the production of transgenetic palnts is that ofA. Escherichia coliB. Bacillu thuringgiensisC. Staphylococcus aureusD. Agrobacterium tumefaciens |
| Answer» Correct Answer - D | |
| 35. |
DNA is enclosed within membranes in an E.coli . Suggest a reagent /molecule that can help to release trapped DNA |
| Answer» Correct Answer - Lysozyme | |
| 36. |
What do you understand by staggered ends ? |
| Answer» These are single-stranded portions seen as overhanging stretches at each end of DNA. They are also known as sticky/cohesive ends. | |
| 37. |
What is trasnformation of bacterial cell ? |
| Answer» It is uptake of DNA of bacterial cells | |
| 38. |
Method involving entry og high velocity gold microparticles coated with DNA into plant cell isA. Protoplast fusionB. TransfectionC. BiolisticsD. Magic bullet action |
| Answer» Correct Answer - C | |
| 39. |
The optimum temperature for polymerisation in PCR is __________ while the enzyme responsible for the mentioned step can tolerate temperature upto ____________. Select the correct option according to the blanks.A. `95^(@) C, 60^(@)C`B. `94^(@)C, 95^(@)C`C. `72^(@)C, 95^(@)C`D. `95^(@)C , 72^(@)C` |
| Answer» Correct Answer - C | |
| 40. |
Assertion : Downstrem processing is generally considered more difficult and coslier in plants than that in microbes. Reason : Rhizosecretion is used as a method to facilitate easier recovery of recombinant proteins from plants. |
| Answer» Correct Answer - B | |
| 41. |
Assertion : The matrix used in gel electrophoresis should have controllable pore size. Reason : Agarose concentration can be changed to change pore sizes. |
| Answer» Correct Answer - B | |
| 42. |
Which of the following microbes transform normal plant and animal cells to cancerous cells respecively ?A. Retroviruses and RhizobiumB. Esherichia coli and Agrobacterium tumfaciensC. Agrobacterium tumefaciens and RetrovirusesD. Agrobacterium tumefaciens and A.rhizogenes |
| Answer» Correct Answer - C | |
| 43. |
Tumor including plasmid transformsA. NematodesB. BacteriaC. FungiD. Several dicot plants |
| Answer» Correct Answer - D | |
| 44. |
A chimaeric DNA is formed byA. Deleting selectable markersB. Joining c-DNA with plasmidC. EcoRID. Enzyme `beta- galactosidase` |
| Answer» Correct Answer - B | |
| 45. |
A plasmidA. Shows dependent assortmentB. Has ability to replicate within bacterial cells independent of the control of chromosomal DNAC. Cannot replicateD. Contains genes for vital activities |
| Answer» Correct Answer - B | |
| 46. |
During insetional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the emzymeA. `alpha`-glucosidaseB. restriction endonucleaseC. `beta`-galactosidaseD. Taq polymerase |
| Answer» Correct Answer - C | |
| 47. |
Restriction endonuclease -A. Cuts the DNA molecules randomlyB. Cuts the DNA molecules at specific sitesC. Restricts the synthesis of DNA inside the nucleusD. Synthesizes DNA |
| Answer» Correct Answer - B | |
| 48. |
Name the reagent that helps in precipitation of DNA from solution. |
| Answer» Correct Answer - Chilled ethanol | |
| 49. |
Which of the following is required to perform polymerase chain reaction ?A. Primers, `dNTPs` and `DNA` polymeraseB. `DNA, CaCI_(2)` and nucleaseC. `Mg^(2+), DNA`D. Both (a) and (c) |
| Answer» Correct Answer - D | |
| 50. |
Which one of the following is not a correct match ?A. Tumour inducing - Ti plasmidB. DNA probe - Identifies the desired DNA fragmentC. PCR - DNA stainingD. Agarose - Sea weeds |
| Answer» Correct Answer - C | |