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101.

Which of the following was not a protein degrading agent used by Noller in his experiment?(a) Phenol(b) SDS(c) Urea(d) Proteinase KI have been asked this question in an international level competition.The origin of the question is Ribosome as Ribozyme in section Gene Expression : Translation of Cytogenetics

Answer»

Right option is (c) UREA

To elaborate: In NOLLER’s experiment ribosomes were STRONGLY TREATED with protein degrading agents like PHENOL, SDS etc; but urea was not one of them.
Discussion
102.

Tetracyclin is only harmful to prokaryotes.(a) True(b) FalseThis question was posed to me during a job interview.My question is taken from Ribosome as Ribozyme in portion Gene Expression : Translation of Cytogenetics

Answer» CORRECT CHOICE is (a) True

To explain: Prokaryotes selectively accumulate tetracycline in their cells by active transport. HOWEVER, HUMANS don’t do so. Thus, tetracycline selectively inhibits BACTERIAL translation.
Discussion
103.

Which of the following is not a stop codon?(a) UAA(b) GUG(c) UAG(d) UGAThe question was asked in quiz.I would like to ask this question from Genetic Code topic in portion Gene Expression : Translation of Cytogenetics

Answer» RIGHT ANSWER is (b) GUG

Explanation: GUG is not a stop codon, rather it is a start codon which can be used in PLACE of AUG. On the other hand UAA, UAG and UGA are stop CODONS.
Discussion
104.

Aminoglycosides can kill_______________(a) Broad spectrum of prokaryotes(b) Only gram positive bacteria(c) Only anerobic gram positive bacteria(d) Only aerobic gram negative bacteriaI got this question in examination.Asked question is from Ribosome as Ribozyme topic in division Gene Expression : Translation of Cytogenetics

Answer»

The correct option is (d) Only aerobic gram NEGATIVE bacteria

To elaborate: AMINOGLYCOSIDES are the group of ANTIBIOTIC that can kill gram negative bacteria which are aerobic. Some of such antibiotics are Streptomycin, PARAMOMYCIN etc.
Discussion
105.

In an experiment you charge a tRNA with a wrong base. You allow the mixture to stand for a while. What will you expect to see?(a) Properly charged tRNA(b) Mischarged tRNA(c) Uncharged tRNA(d) Degraded tRNAI have been asked this question by my school principal while I was bunking the class.This is a very interesting question from TRNA in chapter Gene Expression : Translation of Cytogenetics

Answer»

The correct option is (C) UNCHARGED tRNA

Easy explanation: Certain synthetases can cleave the bond between wrong AMINO ACID and the tRNA. This is a type of proofreading mechanism that ENSURES correct amino acid incorporation.
Discussion
106.

How many proteins were still intact after the treatment of ribosome with SDS and proteinase K?(a) 4(b) 8(c) 12(d) 16The question was asked during an internship interview.This is a very interesting question from Ribosome as Ribozyme topic in chapter Gene Expression : Translation of Cytogenetics

Answer»

Right choice is (b) 8

To explain: Even after such harsh treatment with SDS and PROTEINASE K, 8 proteins were still retained in the RIBOSOME. If they are removed by phenol treatment the peptidyl transfer activity is LOST as they support some tertiary or quaternary structure.
Discussion
107.

Which of these is transcribed by RNA pol III?(a) 28S(b) 16S(c) 5.8S(d) 5SI have been asked this question during an online exam.My question comes from Ribosome in division Gene Expression : Translation of Cytogenetics

Answer»

Correct answer is (d) 5S

For explanation: While most of the EUKARYOTIC rRNAs are transcribed by the RNA POL I, the 5S rRNA is transcribed by RNA pol III. 16S rRNA is HOWEVER absent in EUKARYOTES.
Discussion