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How many proteins were still intact after the treatment of ribosome with SDS and proteinase K?(a) 4(b) 8(c) 12(d) 16The question was asked during an internship interview.This is a very interesting question from Ribosome as Ribozyme topic in chapter Gene Expression : Translation of Cytogenetics

Answer»

Right choice is (b) 8

To explain: Even after such harsh treatment with SDS and PROTEINASE K, 8 proteins were still retained in the RIBOSOME. If they are removed by phenol treatment the peptidyl transfer activity is LOST as they support some tertiary or quaternary structure.



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