InterviewSolution
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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
Which one of the following is used in genetic engineering ?A. RNA polymeraseB. DNA polymeraseC. Restriction endonucleaseD. Nuclease |
| Answer» Correct Answer - C | |
| 52. |
Restriction endonucleases areA. Used in gentic engineering for uniting two DNA moleculesB. Used for in vitro DNA synthesisC. Present in mammalian cells for degeneration of DNA of dead cellsD. Synthesised by bacteria for their defence |
| Answer» Correct Answer - D | |
| 53. |
Advancement in genetic engineering has been possible due to :A. OncogenesB. TransposonsC. Restriction endonucleaseD. Exonucleases |
| Answer» Correct Answer - C | |
| 54. |
Advancement in genetic engineering has been possible due to :A. TransposonsB. EndonucleasesC. ExonucleasesD. Oncogenes |
| Answer» Correct Answer - B | |
| 55. |
Plasmids are used in genetic engineering because they areA. Easily availableB. Able to replicateC. Able to integrate with host chromosomeD. Inert |
| Answer» Correct Answer - B | |
| 56. |
Genetic engineering has been made possible due toA. Observation of DNA under electron microscopeB. We can break DNA at specific points by DNA-asesC. Availability of restriction endonucleases in purified formD. Knowledge of transduction |
| Answer» Correct Answer - C | |
| 57. |
Genetic engineering is :A. Making artificial genesB. Hybridisation of DNAC. Making artifical limbs and diagnostic instrumentsD. Prodcution of alcohol by using microorganisms |
| Answer» Correct Answer - B | |
| 58. |
Bacterium commonly used in plant genetic engineering isA. AgrobacteriumB. CorynebacteriumC. Bacillus subtilisD. Salmonella typhii |
| Answer» Correct Answer - A | |
| 59. |
Two bacterial most useful in genetic engineering areA. Rhizobium and AzotobacterB. Escherichia and AgrobacteriumC. Rhizobium and DiplococcusD. Nitrosomonas and Klebsiella |
| Answer» Correct Answer - B | |
| 60. |
Removal and insertion of genes isA. Genetic engineeringB. BiotechnologyC. Gene therapyD. Cytogenetics |
| Answer» Correct Answer - A | |
| 61. |
The enzymes commonly used in gentic engineering are :A. Restriction endonuclease and polymeraseB. Endonuclease and ligaseC. Restriction endonuclease and ligaseD. Ligase and polymerase. |
| Answer» Correct Answer - C | |
| 62. |
Which enzyme is useful in genetic engineering ?A. DNA - aseB. AmylaseC. LipaseD. Restriction endonuclease |
| Answer» Correct Answer - D | |
| 63. |
Restriction enzymes are used in genetic engineering because theyA. Can join DNA fragmentsB. Cut DNA at specific base sequenceC. Cut DNA at variable sitesD. Are proteolytic enzymes which degrade harmful proteins. |
| Answer» Correct Answer - B | |
| 64. |
Which statement is suitable for `tet^(R)` geneA. It has Pst - I recognition siteB. It has Bam HI and Sal-I recognition sitesC. It has tet III recognition sitesD. None of the above |
| Answer» Correct Answer - B | |
| 65. |
Which is correct match the phenomenon and its explanationA. Central dogma-RNA `rarr` RNA DNA `rarr` Protein `rarr` RNA`B. Reverse transcription - PCR - Many copies of DNA sequenceC. Transcription - Formation of RNA proteinsD. RNA silencing - Use of dsRNA |
| Answer» Correct Answer - B | |
| 66. |
Insect pest resistant Bt Cotton was developed byA. Somaclonal variationB. MicropropagationC. Transgenic technologyD. Somatic hybridisation |
| Answer» Correct Answer - C | |
| 67. |
In order to induce the bacterial uptake of plasmids, the bacteria are made competent by treating them withA. Sodium chlorideB. Potassium chlorideC. Magnesium chlorideD. Calcium chloride |
| Answer» Correct Answer - D | |
| 68. |
Bioreactors are useful inA. Separation and purification of a productB. MicroinjectionC. Processing of large volumes of cultureD. Isolation of genetic material |
| Answer» Correct Answer - C | |
| 69. |
Stirred-tank biroreactors have been designed forA. Purification of the productB. Ensuring anaerobic conditions in the culture vesselC. Availability of oxygen throughout the processD. Addition of preservatives to the product. |
| Answer» Correct Answer - C | |
| 70. |
Satellite DNA is useful tool inA. Sex determinationB. Forensic scienceC. Genetic engineeringD. Organ transplantation |
| Answer» Correct Answer - B | |
| 71. |
Strategy used to prevent nematode infection of tobacco roots isA. Use of agrochemicalsB. Bt toxin geneC. Gene mutationD. RNA interference |
| Answer» Correct Answer - D | |
| 72. |
The most abundant prokaryotes helpful to humans in making curd from milk and in production of antibiotics are the ones categorised asA. ArchaebacteriaB. CyanobacteriaC. Heterotrophic bacteriaD. Chemoautotrophs |
| Answer» Correct Answer - C | |
| 73. |
Restriction enzyme EcoR I cleavages DNA at the sequenceA. AAGCTTB. AAGTTCC. GTATATCD. GAATTC |
| Answer» Correct Answer - D | |
| 74. |
Restriction sites for Pvu I and Pvu II respectively are in which genes of pBR 322A. rop, `amp^(R)`B. `amp^(R)`, ropC. rop, oriD. ori, rop |
| Answer» Correct Answer - B | |
| 75. |
In pBR 322, the gene sequence that codes for the proteins involved in replication of the plasmid isA. Pvu IIB. Cla IC. ropD. pst I |
| Answer» Correct Answer - C | |
| 76. |
It is now possible to breed plants and animals with desired characters throughA. Tissue cultureB. Genetic engineeringC. Ikebana techniqueD. Chromosome engineering |
| Answer» Correct Answer - B | |
| 77. |
Structure involved in genetic engineering isA. PlastidB. Restriction endonucleaseC. DNA polymerase ID. Prochromosome |
| Answer» Correct Answer - B | |
| 78. |
Which of the following is not a characteristic of pBR322 vector ?A. It is the first artificial cloning vector constructed in 1977 by Boliver and RodriguezB. It is most widely used, versatile and easily manipulated vectorC. It has two antibiotic resistance genes, `tet^(R)` and `amp^(R)`D. It does not have restriction site for sal I |
| Answer» Correct Answer - D | |
| 79. |
Match the column and find the correct answer A. a-r, b-s, c-q, d-pB. a-r, b-q, c-s, d-pC. a-q, b-r, c-s, d-pD. a-q, b-s, c-r, d-q |
| Answer» Correct Answer - A | |
| 80. |
Match the column and find the correct combination A. a-2, b-4, c-1, d-5, e-3B. a-2, b-4, c-5, d-1, e-3C. a-4, b-3, c-5, d-2, e-1D. a-4, b-2, c-5, d-3, e-1 |
| Answer» Correct Answer - C | |
| 81. |
DNA or RNA segment tagged with a radioactive molecule is called :A. ProbeB. CloneC. PlasmidD. Vector |
| Answer» Correct Answer - A | |
| 82. |
Plasmids are vectors for gene cloning because theyA. Self replicate in bacterial cellsB. Replicate freely outside bacterial cellsC. Can be multiplied in cultureD. Can be multiplied in laboratories using enzymes. |
| Answer» Correct Answer - A | |
| 83. |
Bacteria protect themselves from viruses by fragmenting viral DNA upon entry withA. MethylaseB. EndonucleasesC. LigasesD. Exonucleases |
| Answer» Correct Answer - B | |
| 84. |
Bacteria protect themselves from viruses by fragmenting viral DNA withA. EndonucleaseB. ExonucleaseC. GyraseD. Ligase |
| Answer» Correct Answer - A | |
| 85. |
Which is related to genetic engineering ?A. PlastidB. PlasmidC. HeterosisD. Mutation |
| Answer» Correct Answer - B | |
| 86. |
A plasmid isA. Lives together with chromosomesB. Shows dependent assortmentC. Can replicate independentlyD. Cannot replicate |
| Answer» Correct Answer - C | |
| 87. |
Restriction endonuclease is used inA. Tissue cultureB. Genetic engineeringC. Cell fractionationD. Regeneration of tissues |
| Answer» Correct Answer - B | |
| 88. |
Enzyme required for polymerase chain reaction (PCR) isA. RNA polymeraseB. RibonucleaseC. Taq polymeraseD. Endonuclease |
| Answer» Correct Answer - C | |
| 89. |
Bt gene is got fromA. Brassica napusB. Bacillus thuringiensisC. AzollaD. Rhizobium |
| Answer» Correct Answer - B | |
| 90. |
Recombinant DNA or r DNA technology was discovered byA. KhoranaB. Bateson and de VriesC. Sutton and AveryD. Cohen and Boyer |
| Answer» Correct Answer - D | |
| 91. |
Genes can be inserted into human cells byA. PCRB. XenotransplantationC. Modified virusesD. Modified microarrays |
| Answer» Correct Answer - C | |
| 92. |
Polymerase chain reactionA. Is a method of synthesising human protein from human DNAB. Uses restriction enzymesC. Can produce billions of copies of a DNA fragmentD. Takes place naturally in bacteria |
| Answer» Correct Answer - C | |
| 93. |
Polymerase chain reaction employsA. Primers and DNA ligaseB. DNA ligase onlyC. DNA polymerase onlyD. Primers and DNA polymerase |
| Answer» Correct Answer - D | |
| 94. |
Identify the plasmidA. EcoRIB. pbR 322C. AIUID. Hind III |
| Answer» Correct Answer - B | |
| 95. |
The technique in which foregin DNA is precipitated over surface of metal particles for passing into target cells isA. MicroinjectionB. ElectroporationC. Particle gunD. Chemical mediated gene transfer. |
| Answer» Correct Answer - C | |
| 96. |
Enzyme that cuts DNA at specific sites isA. DNA ligaseB. Restriction endonucleaseC. DNA polymeraseD. Reverse transcriptase |
| Answer» Correct Answer - B | |
| 97. |
Biolistic technique is used in :A. Tissue culture processB. Hybridisation processC. Germplasm conservation processD. Gene transfer process |
| Answer» Correct Answer - D | |
| 98. |
In hybridoma technolgyA. B-cells are fused with myeloma cellsB. T-cells are fused with myeloma cellsC. T-cells are fused with B-cellsD. None of the above |
| Answer» Correct Answer - A | |
| 99. |
Hybridoma is connected with:A. Growth of cancerB. Monoclonal antibody formationC. Antibody-antigen interactionD. Activity of NK cells |
| Answer» Correct Answer - B | |
| 100. |
Vector for T-DNA isA. Salmonella typhimuriumB. Thermus aquaticusC. Agrobacterium tumefacieneD. Escherichia coli |
| Answer» Correct Answer - C | |