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151.

ElishaA. Uses complement mediated cell lysisB. Uses rediolabelled second antibodyC. Involves addition of substrate which is converted into coloured end productD. Requires RBCs

Answer» Correct Answer - C
Discussion
152.

First step in Southern blot technique isA. Digestion of DNA by restriction enzymeB. Production of a group of genetically identically cellsC. Denaturation of DNA on the gel for hybridisation with specific probeD. Denaturation of DNA from a nucleated cell as from the scene of crime

Answer» Correct Answer - A
Discussion
153.

Read a and b and identify correct choice Statement a. Agrobacterium tumefaciens causes crown gall in dicots Statement b. Agrobacterium tumefaciens enters host through wound and injuriesA. b is correct, a is wrongB. Both a and b are wrongC. Both a and b are correctD. a is correct, b is wrong

Answer» Correct Answer - A
Discussion
154.

A dicotyledonous plant forms crown gall whenA. Agrobacterium tumefaciens comes in contact with the plantB. Agrobacterium rhizogenes comes in contact with the plantC. A specific part of DNA from the Ti plasmid gets integrated with the plant chromosomeD. A specific part from the Ri plasmid gets integrated with the plant chromosome

Answer» Correct Answer - C
Discussion
155.

GAATTC is recognition site of restriction endonucleaseA. Hind IIIB. Eco RIC. BamH ID. Hae III

Answer» Correct Answer - B
Discussion
156.

In the nomenclature of enzyme restriction endonuclease the Roman numerical indicatesA. Number of times it is usedB. The order of discovery from sourceC. Number of cuts on DNAD. Number of recombinants formed

Answer» Correct Answer - B
Discussion
157.

Biopiracy isA. Exploitation of bioresourcesB. Patenting bioresources of othersC. Use of bioresources without authorisationD. Both B and C

Answer» Correct Answer - D
Discussion
158.

Illegal and unlawful development of biomaterials without payment to inhabitants of their region is called:A. BiopatentB. BiotechnologyC. BiowarD. Biopiracy

Answer» Correct Answer - D
Discussion
159.

Main aim of human genome project is toA. Introduce new genes into human beingsB. Identify and sequence all the genes present in human DNAC. Develop better techniques to compare two human DNAsD. Remove disease causing genes from human DNA

Answer» Correct Answer - B
Discussion
160.

Genetically engineered microorganism used successfully in bioremediation of oil spills is:A. TrichodermaB. XanthomonasC. BacillusD. Pseudomonas

Answer» Correct Answer - D
Discussion
161.

Ti-plasmid used in genetic engineering has been modifed byA. Adding tumour forming genesB. Deleting tumour forming genesC. Adding genes for endonucleasesD. Deleting genes for endonucleases

Answer» Correct Answer - B
Discussion
162.

Insect tolerant gene from Bacilus thuringiensis is introduced using Ti plasmid ofA. Escherichia coliB. Agrobacterium tumefaciensC. Haemphilus influenzaeD. Arabidopsis thaliana

Answer» Correct Answer - B
Discussion
163.

In cloning experiments cDNA molecules can be obtained from an mRNA copy byA. Polymerase chain reactionB. Reverse transcriptaseC. RibozymeD. DNA-RNA hybridisation.

Answer» Correct Answer - B
Discussion
164.

With respect to DNA fragmentation Statement A: Gel electrophoresis and elution are two important processes Statement B : After staining with ethidium bromide, it has to be exposed to UV lightA. Only A is correct and B is not correctB. Only B is correctC. Both A and B are correct statementsD. Only A is correct

Answer» Correct Answer - C
Discussion
165.

The process of separation and purificaition of expressed protein before marketing is calledA. Upstream processingB. Downstream processingC. BioprocessingD. Post-production processing

Answer» Correct Answer - B
Discussion
166.

DNA is generally methylated atA. A-baseB. G-baseC. T-baseD. C-base

Answer» Correct Answer - D
Methylation also occurs at A-base.
Discussion