calculate the molecular mass of CO2.

1.	calculate the molecular mass of CO2.
Answer» Solution :(i) Write the dental formula of rabbit. (ii) With a neat labelled diagram explain the techniques involved in gene CLONING. (i) Dental formula is `(1(2)/(1),C(0)/(0),PM(3)/(2),M(3)/(3))` in Rabbit, which is written as `(2033)/(1023)` I - Incisors PM - Premolar C-Canines M - Molars (ii) Gene cloning The carbon copy or more appropriately, a clone means to make a genetically exact copy of an organism. In gene cloning, a gene or a piece of DNA fragment is inserted into a bacterial cell, where DNA will be multiplied (copied) as the cell DIVIDES. Techniques involved in gene cloning: 1. Isolation of desired DNA fragment by using restriction enzymes. 2. Insertion of the DNA fragment into a suitable vector (plasmid) to make rDNA. 3. Transfer of rDNA into bacterial host cell (Transformation). 4. Selection and MULTIPLICATION of recombinant host cell to get a clone. 5. EXPRESSION of cloned gene in host cell.

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