Which of the following is not correct about the X-ray Crystallography?(a) In x-ray protein crystallography, proteins need to be grown into large crystals in which their positions are fixed in a repeated, ordered fashion(b) The protein crystals are illuminated with an intense x-ray beam(c) The x-rays are deflected by the electron clouds surrounding the atoms in the crystal producing a regular pattern of diffraction(d) The protein crystals are illuminated with an intense infrared beamI have been asked this question during an interview.The origin of the question is Protein Structure Basics topic in portion Structural Bioinformatics of Bioinformatics

Which of the following is not correct about the X-ray Crystallography?

1.	Which of the following is not correct about the X-ray Crystallography?(a) In x-ray protein crystallography, proteins need to be grown into large crystals in which their positions are fixed in a repeated, ordered fashion(b) The protein crystals are illuminated with an intense x-ray beam(c) The x-rays are deflected by the electron clouds surrounding the atoms in the crystal producing a regular pattern of diffraction(d) The protein crystals are illuminated with an intense infrared beamI have been asked this question during an interview.The origin of the question is Protein Structure Basics topic in portion Structural Bioinformatics of Bioinformatics
Answer» The correct option is (d) The protein crystals are illuminated with an intense infrared beam The explanation: The diffraction pattern is composed of THOUSANDS of tiny spots recorded on a x-ray film. The diffraction pattern can be CONVERTED into an electron density MAP using a mathematical procedure known as Fourier transform. To interpret a three-dimensional structure from two-dimensional electron density MAPS requires solving the phases in the diffraction data.

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