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Which of the following plot is also known as a double reciprocal plot?(a) Line-weaver burk plot(b) Eadie-Hofstee plot(c) Michaelis-menten plot(d) Langmuir plotThis question was posed to me by my college professor while I was bunking the class.Asked question is from Determining Enzyme Kinetic Constants from Batch Data topic in portion Homogenous Reactions of Bioprocess Engineering

Answer»

Right answer is (a) Line-weaver BURK plot

For explanation I would say: The double-reciprocal equation is obtained by taking the reciprocal of both sides of the Michaelis-Menten equation. The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the SUBSTRATE concentration (1/[S]). The vmax can be accurately DETERMINED and thus KM can also be determined with ACCURACY because a STRAIGHT line is formed. The slope of the resulting line is KM/vmax, the y-intercept is 1/vmax, and the x-intercept is -1/KM.



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