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17551.

Question : DNA is the better genetic material than RNA. Justify थे statement with three comparative reasons.

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Solution :(a) Structurally and chemically DNA is more stable than RNA.
(b) DNA MUTATE slow, but RNA mutate fast.
(C) 2-OH GROUP present in RNA is a reactive group and MAKES RNA labile and easily degradable.
(d) DNA is less reactive than RNA.
(e) Presence of thymine at the place of uracil give additional stability to DNA.
17552.

Question : DNA is not present in

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Ribosomes
Nucleus
Mitochondria
CHLOROPLAST

ANSWER :A
17553.

Question : DNA is generally methylated at

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A-base
G-base
T-base
C-base

Answer :D
17554.

Question : DNA is better genetic material than RNA, because:-

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DNA is chemically less reactive and structurally more STABLE as compared to RNA
Presence of thymine at the PLACE of URACIL provides additional stability to DNA
DNA being stable, so mutate at a SLOW rate
All the above

Answer :D
17555.

Question : DNA is a polymer of nucleotides which are linked to each other by 3' - 5' phosphodiester bond . To prevent polymerisation of nucleotides , which of the following modifications would you choose ?

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Replace PURINE with pyrimidines
Remove / Replace 3' OH group in deoxyribose
Remove / Replace 2' OH group with some other in deoxyribose
Both ( B ) and ( C )

ANSWER :D
17556.

Question : DNA is a polymer of nucleotides which are linked to each other by 3'-5' phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose ?

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Replace purine with pyrimidines
Remove/Replace 3' OH group in deoxy ribose
Remove/Replace 2' OH group with some other group in deoxy ribose
Both (B) and (C)

Solution :Remove/Replace 3. OH group in deoxy ribose
DNA is a polymer of NUCLEOTIDES which are LINKED to each other by 3.-5. phosphodiester bond. Remove/replace 3.OH group in deoxyribose, to PREVENT polymerisation of nucleotides.
17557.

Question : DNA is a polymer of nucleotides which are linked to each other by 3' - 5' phosphodiester bond. To prevent polymerisation of nucle- otides, which of the following modifications would you choose

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Replace PURINES with pyrimidines
Remove/Replace 3' OH group in deoxyribose
Remove / Replace 2' OH group with some other group in deoxyribose
Both (B) and ©

Answer :b
17558.

Question : DNA is a negatively charged molecule, hence it moves towards the

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CATHODE
ANODE
EQUATOR
all of these

ANSWER :B
17559.

Question : DNA is a hydrophilic molecule, so it cannot pass through cell membranes. Then how does a recombinant DNA inserted into a plasmid vector, enter into a bacterial cell ?

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Solution :This is done by treating them with a specific concentration of a divalent cation such as calium, which increases the EFFICIENCY with efficiency with which DNA enters the bacterium through pores in its cell WALL. Recombinant DNA canbe then forced into such cells by incubating the cells with recombinant DNA on ice, FOLLOWED by placing them briefly at `42^(@) C` and then putting them back on ice. This enables the BACTERIA to take up the recombinantDNA .
17560.

Question : DNA is a hydrophilic molecule, it cannot pass through cell membrane. In order to force bacteria to take up the plasmid, the bacterial cells must be made competent to take up DNA. Write the procedure for making the cell competent?

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Solution :Treating host with a specific CONCENTRATION of a divalent cation such as calcium.
Which increases the EFFICIENCY with which DNA enters the bacterium through pores in its CELL wall.
Recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice followed by placing them briefly at `42^@`C (heat shock).
Then PUTTING them back on ice. this ENABLES the bacteria to take up the recombinant DNA.
17561.

Question : DNA is

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Cestrum
Cucumber
Potato
Radish.

Answer :B
17562.

Question : DNA in eukaryotes is a double helix and:

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COMPLEMENTARY and parallel
complementary and ANTIPARALLEL
WITHOUT SUPERCOILS
always circular

Answer :B
17563.

Question : DNA fungerprinting rfers to :-

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Techniques used for indentification of FINGERPRINTS of individuals
MOLECULAR analysis of profiles of DNA SAMPLES
Anylsis of DNA samles using imprinting DEVICES
Techinques used for molecular analysis of different specimens of DNA

Answer :B
17564.

Question : DNA-Gyrase enzyme, that participates in the process of DNA-replication is a type of :-

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DNA ligase
DNA topoisomerase
Reverse transcriptase
DNA polymerase

Answer :B
17565.

Question : DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

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CENTRIFUGATION
POLYMERASE CHAIN reaction
electrophoresis
restriction mapping

Solution :electrophoresis
17566.

Question : DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

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POLYMERASE CHAIN reaction
Electrophoresis
Restriction mapping
Centrifugation

Answer :A
17567.

Question : DNA fragments can be separated and detected using_____.

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ANSWER :ELECTROPHORESIS
17568.

Question : DNA fragments are joined in a correct sequence by

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DNA POLYMERASE I
DNA ligase
RNA polymerase
Helicase

Answer :A
17569.

Question : DNA fragments are :

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NEGATIVELY charged
Nertral
Positively charged
Positively or negatively charged

Answer :A
17570.

Question : DNA fingerprinting technique was discovered by

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Wilmut
Jefferys
Eithoven
Kary Mullis

Answer :B
17571.

Question : DNA fragment containing gene of interset to be cloned _______

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SOLUTION :N/A
17572.

Question : DNA fingerprinting technique was first developed by :

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Jeffreys, WILSON and Thein
Schleiden andSchwann
Edward and Steptoe
Boysen and Jensen

Answer :A
17573.

Question : DNA fingerprinting is used for patenity testing.

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Solution :REASON:DNA patermity TESTING is the USE of DNA biplogically PARENT of the child in the case opf a disputes.
17574.

Question :DNA fingerprinting refers to(A) Molecular analysis of profiles of DNA samples(B) Analysis of DNA samples using imprinting device(C) Technique used for molecular analysis of different specimens of DNA(D) Techniques used for identification of fingerprints of individuals

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MOLECULAR analysis of profiles of DNA samples
Analysis of DNA samples USING IMPRINTING device
Technique used for molecular analysis of different specimens of DNA
Techniques used for identification of fingerprints of individuals

Solution :Molecular analysis of profiles of DNA samples
17575.

Question : DNA fingerprinting method is very useful for

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DNA tests for IDENTITY and relationships
FORENSIC studies
Polymorphism
All of the above

Answer :D
17576.

Question : DNA fingerprinting is related to :

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MOLECULAR ANALYSIS of profiles of DNA SAMPLES
Analysis of DNA samples using imprinting devices
Techniques used for molecular analysis of different specimens of DNA
Techniques used in indentification of FINGERPRINTS of different persons

Answer :A
17577.

Question : DNA fingerprint is associated with …………………

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to molecular ANALYSIS of DNA prints or IMAGES.
to analysis tech. for DNA printing.
for different DNA model by molecular analysis tech.
for individual fingerprint.

SOLUTION :to molecular analysis of DNA prints or images.
17578.

Question : DNA finger printing was invented by :-

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KARY Mullis
Alec Jeffery
Dr. PAUL Berg
Francis Collins'

Answer :B
17579.

Question :DNA finger printing technique is based on

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RESTRICTION endonuclease
polymorphism in REPETITIVE DNA
Gel electrophoresis
Southern BLOTTING technique

Answer :B
17580.

Question : DNA finger printing is useful in

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Taxonomical APPLICATIONS
PEDIGREE analysis
Anthropological studies
All the THREE

ANSWER :D
17581.

Question : DNA finger printing refers to :

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Molecular ANALYSIS of profiles of DNA samples
Analysis of DNA samples using imprinting device
Techniques USED for molecular analysis of DIFFERENT specimens of DNA'
Techniques used for identification of finger PRINTS of individuals

Answer :A
17582.

Question : DNA finger printing involves

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IDENTIFICATION of differences in all REGIONS of DNA sequence
Identification of differences in some SPECIFIC regions in DNA sequence
Identification of similarities in any region of DNA sequence
NONE of these

Answer :B
17583.

Question : DNA finger printing is the basis of peternity testing in case of disputes. Why ?

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SOLUTION :GENETIC POLYMORPHISMS are INHERITABLE from peresents to CHILDREN.
17584.

Question : DNA finger printing has application in the field of

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FORENSIC science
Genetic Biodiversity
Evolutionary biology

Answer :D
17585.

Question : DNA finger printing involves the use of

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CDNA
MRNA
r-RNA
t-RNA

ANSWER :A
17586.

Question : DNA finger print profile will be exactly same of :

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Siblings
Offsprings
IDENTICAL twins
Fraternal twins

Answer :C
17587.

Question : DNA element with ability to change positions is called

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Cistron
TRANSPOSON
Intron
Recon

ANSWER :B
17588.

Question : DNA element with ability to change its position is called :

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CISTRON
TRANSPOSON
INTRON
Recon

Solution :Transposon
17589.

Question : DNA duplication takes place during :

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only in S phase
entire interphase
only in `G_1` phase
Only in `G_2` phase

Answer :A
17590.

Question : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the

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TEMPLATE STRAND
CODING strand
alpha strand
anti strand

SOLUTION :template strand
17591.

Question : DNA dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the

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ALPHA strand
ANTI strand
Template strand
CODING strand

ANSWER :C
17592.

Question : DNA content in a meiocyte at G_2 phase is 40 pg. What would be the DNA content after meiosis-II in the daughter cells?

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20 pg
5 pg
10 pg
15 pg

Answer :C
17593.

Question : DNA dependent DNA polymerases catalyse polymerization of DNA in

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Only 3. `to`5. direction
Only 5. `to`3.direction
Both direction
Irregular directions

Answer :B
17594.

Question : DNA contains nucleobasės , sugars and phosphate . Removal of which among these from a DNA sample will not significantly affect the length of DNA ?

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NUCLEOBASES
SUGAR
PHOSPHATE
NONE of these

Answer :A
17595.

Question : DNA contains nucleobases, sugar and phosphate. Removal of which among these from a DNA sample will not significantly affect the length of DNA?

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Nucleobases
Sugar
Phosphate
None of these

ANSWER :A
17596.

Question : DNA caused the Discuss the significance of heavy isotope of nitrogen in the Meselson and Stahl's experiment.

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Solution :Matthew Meselson and Franklin Stahl performed the following experiment in 1958. They grew E. coli in a medium CONTAINING `""^(15)NH4Cl (""^(15)N)` is the heavy isotope of nitrogen) as the only nitrogen source for many generations. The result was that `""^(15)N` was incorporated into NEWLY synthesised DNA (as WELL as other nitrogen containing compounds).
This heavy DNA molecule could be distinguished from the normal DNA by CENTRIFUGATION in a cesium chloride (CSCI) density gradient `""^(15)N` is not a radioactive isotope, and it can be separated from `""^(14)N` only based on densities).
17597.

Question : DNA can be cut with the help of

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DNAase
RNAase
KNIFE
Restriction ENZYME

SOLUTION :Restriction enzyme
17598.

Question : DNA as an acidic substance present in nucleus was first identified by ______ in 1869, he namedit as _________

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Meischer , nuclein
Watson and CRICK , DNA
Chargaff, nuclein
Wilkins and FRANKLIN, DOUBLE helix

Solution :(a)
17599.

Question : DNA and RNA differ in:

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SUGAR only
sugar and phosphate
sugar and purine
sugar and pyrimidine

Answer :D
17600.

Question :DNA and RNA differ by

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N - BASES and PHOSPHATE groups
Number of C - ATOMS in sugars
N - bases and sugars
Sugar and phosphate groups

Answer :C