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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
What might be an advantage of beginning gene therapy prior to birth ?A. This would give the body plenty of time to utilise the new genesB. The body would not reject it as it has not yet recognised selfC. The cells being extremely young, are mor receptive of gene therapyD. There probably is not any advantage |
| Answer» Correct Answer - B | |
| 52. |
ADA deficiency results inA. incresed risk of infertilityB. inalbility of immune system of function normallyC. chromosomal disrodersD. decrese in yied of crop plants |
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Answer» Correct Answer - B ADA deficeency results in inability of immulne system to funciton normally |
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| 53. |
An abnormal gene is replaced by normal gene it is calledA. gene therapyB. cloningC. mutationD. none of the above |
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Answer» Correct Answer - A Alkn abonormal gene is replaced by normal gene it is called gene therapy |
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| 54. |
Given figure represent the maturtion of pro-insulin into insulin. Identify the product A A. Polypeptide chain AB. Polypeptide chain BC. Polypeptide chain CD. none of these |
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Answer» Correct Answer - C A represents polypeptide chain C which is removed prior to insulin formation |
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| 55. |
Which of the following statements regarding the structure of proinsulin and mature insulin are not correct ? (i) Proinsulin is made up of three polypeptide chains -A,B and C (ii) c- polypeptide chain with 33 amino acids is removed prior to insulin formation (iii) Mature insulin is made up of 51 amino acids arranged in two polypeptide chain -A and B (iv) Polypeptide chain A has 30 amino acids and polypeptide chain B has 21 amino acids (v) Polypeptide chains A and B are interconnected by only one S-S linkage.A. (i) and (ii)B. (iii) and (iv)C. (iv) and (v)D. (iii),(iv) and (v) |
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Answer» Correct Answer - A Human insulion is made up of of 51 amino acids arranged in two polypeptide chains : A, having 21 amono acids and B, with 30 amino acids. The two polypeptide chains are interconnected by two disulphide bridges |
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| 56. |
What are Cry proteins? Name an organism that produces it. How has man exploited this protein to his benefit? |
| Answer» Cry proteins are encoded by cry genes. These proteins are toxins, which are produced by Bacillus thuringiensis bacteria. This bacterium contains these proteins in their inactive from. When the inactive toxin protein is ingested by the insect, it gets activated by the alkaline pH of the gut. This results in the lysis of epithelial cell and eventually the death of the insect. Therefore, man has exploited this protein to develop certain transgenic crops with insect resistance such as Bt cotton, Bt corn, etc. | |
| 57. |
An example of gene therapy isA. production of injectible hepatities B vaccineB. production of vaccines in food crops like potatoes which can be eatenC. introduction of gene for adenosine deaminase in persons suffering from SCIDD. production of test tube babies by artificial insemination and implantation of fertilised eggs |
| Answer» Correct Answer - C | |
| 58. |
Some of the steps involved in production of humulin are given below. Choose the correct sequence (i) Synthesis of insulin gene artificially (ii) Culturing recombinant E. coli in bioreactors (iii) Purification of humulin (iv) Insertion and human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.A. ii,iiv,iii,v,viB. I,iv,vii,vi,iiiC. I,iii,v,vi,ii,ivD. iii,v,ii,vi,iv |
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Answer» Correct Answer - B Some of the steps involved in porduciton of humulin are given below .The correct sequence are : (i) Synthisis of insulin g gene artificially (iv) insertion of human insulin gene in to plasmid (v) introduction of recombinant plasmid in to e coli (ii) extraction of recombinant gene product from e coli (iii) pruification of humulin (human insulin) |
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| 59. |
Which of the following companies started selling humulin in the year 1983 ?A. Eli LillyB. GenetechC. GEACD. none of these |
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Answer» Correct Answer - A In 1983, Eli Lilly an American company, first prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of Escherichia coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin (humulin) |
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| 60. |
Which organism is used to transfer T-DNA?A. streptomyces hygroscopicusB. agrobacterium tumefaciensC. salmonella tyuphiD. escherichia coli |
| Answer» Correct Answer - B | |
| 61. |
Giant mouse has been produced throughA. gene transferB. gene differentiationC. tissue cultureD. all of these |
| Answer» Correct Answer - A | |
| 62. |
Genetic engineerign has been successfully used for porducingA. transgenic models for studing new treatment fo r certain caridac diseasesB. transgenic cow rosie which produce high fat milk for making gheeC. animals like bulls for farm work as they have super powerD. transgenic mice for testing safety of polio vaccine before use in humans |
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Answer» Correct Answer - D Genetic engineering has been successfully used for producing transgenic mice for testing safety of olio vaccine befor euse in humans |
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| 63. |
Some of the step involved in the production of humulin are given below. Arrange them in the correct sequence and select the correct option (i) Synthesis of gene (DNA) for human insulin artifically (ii) Culturing recombinant E.coli in bioreactors (iii) Purification of humulin (iv) Insertion of human insulin gene into plasmid (vi) Etraction of recombinant gene product from E.coli.A. (ii),(i),(iv),(iii),(v),(vi)B. (i),(iii),(v),(vi),(ii),(iv)C. (i),(iv),(v),(ii),(vi),(iii)D. (iii),(v),(ii),(i),(vi),(iv) |
| Answer» Correct Answer - C | |
| 64. |
Given below are certain features of mouse. Read them and select why mouse is the most preferred animal for studies on gene transfer (i) Short oestrous cycle and gestation period (ii) Relatively short generation time (iii) Covenient in vitro fertilisation (iv) Production of several offspring per pregnancyA. (i) and (ii) onlyB. (i) onlyC. (i),(ii) and (iv) onlyD. (i),(ii),(iii) and (iv) |
| Answer» Correct Answer - D | |
| 65. |
The transgenic animals are those which haveA. foreign RNA in all its cellsB. foreign DNA in some of its cellsC. foreign DNA in all its cellsD. both (a) and (c) |
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Answer» Correct Answer - C Transgenic organism is one that has become transformed following the introduction of novel genes into its genome. It is most frequency achieved by integration of cloned DNA sequences following their injection into the fertilised egg. The fertilised egg divides mitotically to form the whole organism so that all the cells of the organism will carry the transferred genes. the transferred genes are known as transgenes. |
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| 66. |
Bollworms attacksA. bt cottonB. tomatoC. cottonD. bacillus thuringinensis |
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Answer» Correct Answer - C Bollworm attacks cotton |
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| 67. |
Assertion: Bt toxin is coded by a gene named cry Reason : Bt toxin is coded by a gene named cryA. If both assertion and reason are true and the reason is the correct explanation of the assertionB. If both assetion and reason are true but reason is not the correct explantion of the assertionC. If assertion is true but reason is falseD. If both assertion and reason are false |
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Answer» Correct Answer - B Bt toxins are insect group specific. Bt toxin is coded by a gene named cry |
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| 68. |
Read statement a-d which two of them have mistakes?A. first transgenic buffalo rosie produced milk which was human aphalactalbumin enrichedB. restriction enzymes are used in isolation of DNA from other macormoleculedC. downstream processing is one of the steps of rDNA technologyD. disarmed pathogen vectors are also used in transfter or rDNA in to the host |
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Answer» Correct Answer - D First tansgenic cow rosie produced milk which was human alphalactalbumin enriched Restriction enzymes are used in digestion of DNA |
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| 69. |
Maximum number of existing transgenic animals is ofA. cowB. pigC. miceD. fish |
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Answer» Correct Answer - C Maximum number (95%) of existing transgenic animals of mice |
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| 70. |
Animals that have had their DNA manipulated to possess and express a foreign are calledA. transgenic animalsB. somatic hybridsC. somaclonesD. super animals |
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Answer» Correct Answer - A The animals which carry foreign genes are called transgenic animals. The foreign genes inserted into the genome of the animal using recombinant DNA technology are called transgenes. |
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| 71. |
Give the two name of cry gene which control the cotton bollworms :- |
| Answer» Correct Answer - cry I Ac, cry II Ab | |
| 72. |
Silencing of mRNA/RNA interference has been used in development of plants resistant toA. virusesB. insectsC. fungiD. nematodes |
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Answer» Correct Answer - D Silencing of mRNA/RNA interference has been used in development of plants resistant to nemotodes |
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| 73. |
Which of the following is not a genetically modified organism (GMO) ?A. Golden riceB. RosieC. DogieD. Dolly |
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Answer» Correct Answer - D Golden rice, Rosie and Dogie all are genetically modified organisms. Dolly, the sheep is a cloned animal. |
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| 74. |
which is correct about genetically modified sugar by america?A. obtained patent of bacterial germplasmB. brazzein obtained form gymnema sylverstre is used in maizeC. protein obtained from lantana plant of africa is used in maizeD. brazzein protein obtained from a frican plant is used in maize. |
| Answer» Correct Answer - D | |
| 75. |
NaCl is harmful to most crop plants. A scientist at the University of Toronto genetically modified a plant so that it could be grown in dry parts of the world where the available water has a high level of NaCl. This genetically modified plant copes with the high levels of NaCl by transporting salt into its vacuoles where it accumulated to abnormally high level. which feature would be observed in the genetically modified plant when compared to a non-modified plant ?A. The leaves in the modified plant are more yellow in colourB. The modified plant has salt srystals on the surface of its levesC. The cytosol (the materal between the plasma membrane and the vacuole membrane, excluding the organelles) in the modified plant has a lower osmotic pressureD. The cytosol in the modified plant has a higher osmotic pressure |
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Answer» Correct Answer - D Osmotic pressure is the pressure required to stop the flow of pure water or solvent into a solution across a partially permeable membrane. Water moves from a region of low osmotic pressure to a region of high osmotic pressure. As high levels of NaCl is accumulated in the cells of modified plant. the osmotic pressure of the cytosol is increased. Accumulation of salts in vacuoles causes compartmentalisation of salt away from cytosol and protects cytosol form harmful effects of the high salt concentration. |
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| 76. |
Some of the characteristics of Bt cotton areA. medium yield long fibre and resistance to beetle pestsB. highyield and prodcution of toxic protein crystals which kill dipteran pestsC. high yield and resistance to bollowrmsD. long fibre and resistacne to aphids |
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Answer» Correct Answer - B Some of the charcteristics of Bt cotton are high yield and production of toxic portien cyrtals which kill dipteran pests |
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| 77. |
Cultivation of Bt Cotton has been much in the news . The prefix "Bt" meansA. barium treated cotton seeds.B. bigger thread variety of cotton with better tensile strengthC. produced by biotechnology using restirction enzymes and ligasesD. carrying an endotoxin gene from bacillus thuringiensis |
| Answer» Correct Answer - A | |
| 78. |
Some of the characteristics of Bt cotton areA. long fibre and resistance to aphidsB. medium-yield, long fibre and resistance to beetle pestsC. high yield and production of toxic protein crystals which kill dipteran pestsD. high yields and resistance to bollworms |
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Answer» Correct Answer - D Transgenic Bt cotton, which produces its own insecticide contains a gene from a soil bacterium (Bacillus thuringiensis). Bt Cotton is usually resistant to bollworm disease of cotton |
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| 79. |
Bt cotton is notA. a GM plantB. insect resistantC. a bacterial gene expressing systemD. resistant to all pesticides |
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Answer» Correct Answer - D Several transgenic plants have been developed. One such plane is Bt cotton in which the Bt toxin has been used as a biological insecticide The bacterium-Bacillus thuringiensis is common soil bacteria which produces a protein toxin that kills certain insects. Bt toxin gene was isolated from Bacillus thuringiensis and was incorporated into several crops plant such as cotton. Bt cotton is resistant to insect but is not pesticide resistant. |
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| 80. |
Which of the following types of ELISA contain the following steps ? Antigen binding,Blocking, primary antibody,Secondary antibody,Enzyme-linked antibody, Substrate,Colorimetric reading (Represented in diagram) A. Direct ELISAB. Indirect ELISAC. Competitive ELISAD. Sandwich ELISA |
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Answer» Correct Answer - D Sandwich ELISA is a less common variant of ELISA but is highly efficient in sample antigen detection. The sandwich ELISA quantify antigens between two layers of antibodies. The antigen to be measured must contain atleast two epitopes capable of binding to two antibodies. The basic steps involved are as follows : (i) Plate is coated with captured antibody (ii) Sample is added, and any antigen present in the sample binds to captured antibody (iii) Detecting antibody is added, and binds to antigen (iv) Enzyme-linked secondary antibody is added that binds to detecting antibody (v) Substrate is added which gets converted by enzyme to detectable colour product that can be measured through colorimeter. |
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| 81. |
Assertion : ELISA test is based on antigen-antibody interactions where a pathogen can be detected by the presence of antibodies (proteins,glycoproteins, etc) on it Reason : The pathogen antibody to be identified is immobilised on the surface of specially constructed ELISA plates and is then tested.A. If both assertion and reason are true and reason is the correct explanation of assertionB. If both assertion and reason are true but reason is not the correct explanation of assertionC. If assertion is true but reason is falseD. If both assertion and reason are false |
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Answer» Correct Answer - D ELISA (enzyme linked immuno-sorbant) is a technique based on the principle of antigen-antibody interactions. Pathogens have specific antigens on their surface like protein, glycoproteins, etc. Thus,their infection can be detected by using the antibodies specific to bind with those antigenes. In process antigens are immobilised to ELISA plates. Then antibody specific to the antigen is added and allowed to react with the adsorbed antigen. A complex procedure is followed. |
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| 82. |
Assertion: PCR is a powerful technique to identify many genetic disroder Reason : ELISA is based on the principal of antigen antibody interactionA. If both assertion and reason are true and the reason is the correct explanation of the assertionB. If both assetion and reason are true but reason is not the correct explantion of the assertionC. If assertion is true but reason is falseD. If both assertion and reason are false |
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Answer» Correct Answer - B PCR is a powerful technique ot identify many genetic disorders ELISA is based on the principle of antigen antibody interaction |
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| 83. |
Assertion: Insulin used in diabetes was earlier extracted from pancreas of slaughtered cattle and pigs Reason: Insulin from an animla source caused some patients to develop allergy or other types of reactions to the foreign proteinA. If both assertion and reason are true and the reason is the correct explanation of the assertionB. If both assetion and reason are true but reason is not the correct explantion of the assertionC. If assertion is true but reason is falseD. If both assertion and reason are false |
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Answer» Correct Answer - B Insuling used in diabetes was earlier extracted from pancreas of slaughtered cattle and pigs Insulin form an animal source caused some patient to develop allergy or other types of reaction to the foreign protein |
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| 84. |
Which is the correct match ?A. Cry I ab - cortton bollwormsB. Cry I ac - cotton bollowrmsC. Cry I Ac - corn borerD. Cry II Ab -corn borer |
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Answer» Correct Answer - B The proteins encoded by the genes cryAc and cyIIAb control the cotton bollworms that of cryIAb controls corn borer |
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| 85. |
Insect pest resistant Bt cotton was developed byA. somaclonal variationB. micropropagationC. transgenic technologyuD. somatic hybridisation |
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Answer» Correct Answer - C Insect pest resistant bt cotton was developed by transgeinci technology |
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| 86. |
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA ?A. Adenosine DeoxyaminaseB. Adenosine deaminaseC. Aspartate deaminaseD. Arginine deaminase |
| Answer» Correct Answer - B | |
| 87. |
this is not a GMO:A. golden riceB. tracyC. bt brinjalD. dolly |
| Answer» Correct Answer - D | |
| 88. |
Read the given statements and select the correct option Statement 1 : PCR technique is helpful in detecting bacterial and viral diseases even when symptoms of the disease are not yet visible Statement 2 : Very low concentrations of bacteria or viruses in human body can be detected by amplification of their nucleic acids using the PCR techniqueA. Both statements 1 and 2 are correctB. Statement 1 is correct but Statement 2 is incorrectC. Statement 1 is incorrect but Statement 2 is correctD. Both statements 1 and 2 are incorrect |
| Answer» Correct Answer - A | |
| 89. |
Molecular probes are used for many genetic disorder likeA. Duchenne muscular dystrophyB. cystic-fibrosisC. Tay-Sachs diseaseD. all of these |
| Answer» Correct Answer - D | |
| 90. |
Technique used to detect the DNA in a clone isA. polymerase chain reactionB. gel electrophoresisC. chromatographyD. autoradiography |
| Answer» Correct Answer - A | |
| 91. |
Read the following statements regarding ELISA and select the incorrect oneA. It is used for the early diagnosis of diseasesB. It is based on the principle of antigen-antibody interactionC. Infection by pathogen can be detected the presence of antigenes like proteins and glycoproteinsD. none of these |
| Answer» Correct Answer - D | |
| 92. |
The site of production of ADA in the body isA. erythrocytesB. lymphocytesC. blood plasmaD. osteocytes |
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Answer» Correct Answer - B Lymphocytes are a kind of white blood cells present in bone marrow ADA (adenosine deaminase) is an enzyme that is present in lymphocytes and is very important for the immune system to function. |
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| 93. |
Choose the correct option regarding retrovirusA. An RNA virus that can synthesise DNA during infectionB. A DNA virus that can synthesise RNA during infectionC. A ssDNA virusD. A dsRNA virus |
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Answer» Correct Answer - D A retrovirus is an ss RNA virus that stored its nucleic acid in the form of a mRNA genome and targets a host cell as an obligate parasite. One inside the host cell cytoplasm the virus uses its own reverse transcriptase enzyme to produce DNA from its RNA genome (the reverse of usual pattern, thus retro). |
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| 94. |
A probe which is a molecule used to locate specific sequence in a mixture of DNA or RNA molecules could beA. a single stranded RNAB. a single stranded DNAC. either RNA or DNAD. can be ssDNA but not ssRNA |
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Answer» Correct Answer - C The molecular probes are usually single stranded pieces of DNAs (sometimes RNAs) labelled with radio isotopes such as phosphorus-32. Molecular probes are available for many genetic disorders such as Duchenne muscular dystropy, cystic fibrosis, Tay-Sachs disease. |
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| 95. |
Bacterial artificial chromosomes (BACs), cosmids, phages, plasmids and yeast artifical chromosomes (YACs) are all commonly used cloning vectors that differ in their cloning capacities, with a range from approximately 100 bp to 1000 kb. Which of the following is the correct order for these vectors in terms of increasing cloning capacity ?A. BAC,cosmid,phage,plasmid,YACB. YAC,BAC,cosmid,phage,plasmidC. Plasmid,phage,cosmid,BAC,YACD. Plasmid,cosmid,phage,BAC,YAC |
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Answer» Correct Answer - C Plasmid-generally upto 15 Kb long DNA fragment Phage-up 23 Kb long DNA fragments Cosmid-uptp 45 Kb long DNA fragments BAC-upto 300-350 Kb long DNA fragments YAC-upto 1 Mb long DNA fragments |
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| 96. |
Bt toxins areA. intracellular lipidsB. intracellular crystaline proteinsC. extracellular crystaline proteinsD. intracellular polysaccharides |
| Answer» Correct Answer - B | |
| 97. |
Fill in the blanks B. thuringiensis form a protein crystal during a particular ____(a)______these crystals contain a toxic_______(b)________ |
| Answer» Correct Answer - (a) Phase of their growth (b) Insecticidal protein | |
| 98. |
Fill in the blanks The activated Bt toxin binds to the _______(a)_______and create _______(b)______ |
| Answer» Correct Answer - (a) Midgut epithelial cell (b) Pores | |
| 99. |
The bacterium bacilllus thuringiensis is widely used in contemporary biology asA. source of industrial enzymeB. insecticideC. indicator of water pollutionD. agent for production of dairy products |
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Answer» Correct Answer - B The bacterium bacilllus thuringiensis is widely use din contemporary biology as insecticide |
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| 100. |
What is true of Bt toxin?A. the concerned bacillus ahs antitoxisB. the inacative protoxin gets converted in to active form in the insect gutC. bt protein exist as active toxin in the bacillusD. the activated toxin enters the ovaries of the pest to sterilize it and thus prevents its multiplication |
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Answer» Correct Answer - B Bt toxin is the inactive protoxin gets converted in to active form in the insect gut |
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