70 + Interview Questions in Dna-Based Information Technologies in Biochemistry Page 1 InterviewSolution

1.	Short sub-sequence of a cDNA sequence is ____________(a) Expressed sequence tag(b) Sequence tagged site(c) Contig(d) YACThe question was posed to me by my college director while I was bunking the class.This interesting question is from From Genes to Genomes-1 topic in portion DNA-Based Information Technologies of Biochemistry
Answer» The correct option is (a) Expressed sequence tag Explanation: Sequence tagged site is a SHORT DNA sequence having SINGLE occurrence in genome. Cotig is a set of overlapping DNA SEGMENTS that together represent a consensus REGION of DNA. YAC is a linear DNA molecule.

Discussion

2.	Which of the following is helpful in distinguishing DNA of one individual from another?(a) PCR(b) Reverse transcriptase(c) cDNA(d) RFLPThis question was posed to me in an online interview.This question is from From Genes to Genomes-1 in portion DNA-Based Information Technologies of Biochemistry
Answer» Right answer is (d) RFLP Best EXPLANATION: Restriction fragment LENGTH POLYMORPHISM (RFLP) is HELPFUL in DISTINGUISHING one individual from another.

Discussion

3.	Where does a restriction endonuclease cuts DNA?(a) Ligation site(b) Recognition sequence(c) Ori(d) Replication siteThis question was posed to me during a job interview.This intriguing question originated from DNA Cloning: The Basics topic in section DNA-Based Information Technologies of Biochemistry
Answer» The correct choice is (b) Recognition sequence Explanation: RESTRICTION ENZYMES CUT DNA at specific sites called recognition sites.

Discussion

4.	Which of the following statements is true?(a) Exonuclease III removes nucleotide residues from the 3’ ends of a DNA strand(b) Bacteriophage lambda exonuclease removes terminal phosphates(c) Alkaline phosphatase removes nucleotides from 5‘ends(d) Kinase adds homopolymer tails to the 3’ –OH ends of a linear duplexI had been asked this question during an online interview.Origin of the question is DNA Cloning: The Basics-1 in chapter DNA-Based Information Technologies of Biochemistry
Answer» Correct answer is (a) Exonuclease III removes NUCLEOTIDE residues from the 3’ ends of a DNA strand Explanation: BACTERIOPHAGE lambda exonuclease removes nucleotides from 5‘ends. Alkaline phosphatase removes terminal phosphates Terminal transferase ADDS homopolymer TAILS to the 3’ –OH ends of a linear duplex.

Discussion

5.	The process of introduction of foreign DNA into an animal cell is called ____________(a) Transversion(b) Conversion(c) Inversion(d) TransfectionThis question was addressed to me in final exam.This interesting question is from From Genes to Genomes-1 in portion DNA-Based Information Technologies of Biochemistry
Answer» Correct ANSWER is (d) TRANSFECTION The EXPLANATION: Transfection is the deliberate inducing of foreign DNA into an animal CELL.

Discussion

6.	Gene therapy is a technique preferred to cure inherited diseases by __________(a) Repairing the faulty gene(b) Introducing the correct copy of the gene(c) Adding new cells to the body(d) PCRI have been asked this question during an online exam.My question is taken from Genome Alterations and New Products of Biotechnology in section DNA-Based Information Technologies of Biochemistry
Answer» Correct choice is (b) Introducing the correct COPY of the gene To EXPLAIN I WOULD say: By INSERTING a gene in place of defective ones in order to correct genetic disorders is INCLUDED in gene therapy.

Discussion

7.	Genes of same species, similarly related to each other are __________(a) Ortholog(b) Synteny(c) Paralog(d) MicroarrayI have been asked this question by my college director while I was bunking the class.My enquiry is from From Genomes to Proteasomes in division DNA-Based Information Technologies of Biochemistry
Answer» Correct answer is (c) Paralog The explanation is: Genes of different SPECIES but possessing a clear SEQUENCE and FUNCTIONAL RELATIONSHIP to each other are orthologs. Synteny is a conserved gene order. Collections of MICROSCOPIC DNA spots attached to solid surface are DNA microarrays.

Discussion

8.	Rauolfia serpentine, to save this plant under the threat of extinction, which of the following techniques is useful?(a) Genetic engineering(b) In vitro culture(c) DNA fingerprinting(d) Hybridoma technologyI got this question in final exam.This interesting question is from Genome Alterations and New Products of Biotechnology in chapter DNA-Based Information Technologies of Biochemistry
Answer» RIGHT answer is (b) In VITRO culture Explanation: In vitro culture is useful in SAVING this plant from EXTINCTION.

Discussion

9.	Genes of different species but possessing a clear sequence and functional relationship to each other are __________(a) Ortholog(b) Synteny(c) Paralog(d) MicroarrayI got this question during an interview for a job.I would like to ask this question from From Genomes to Proteasomes topic in portion DNA-Based Information Technologies of Biochemistry
Answer» Correct option is (a) Ortholog The best explanation: Collections of MICROSCOPIC DNA spots attached to SOLID surface are DNA microarrays. Genes of same species, similarly related to each other are paralogs. Synteny is a CONSERVED gene ORDER.

Discussion

10.	What is the goal of structural proteomics project?(a) To crystallize and determine the structure of as many proteins as possible, in many cases with little or no existing information about protein function(b) To identify and sequence of all the genes present in the human body(c) To introduce new genes to human beings(d) To remove disease causing genes from humansThis question was posed to me by my school teacher while I was bunking the class.Origin of the question is From Genomes to Proteasomes topic in division DNA-Based Information Technologies of Biochemistry
Answer» Right choice is (a) To crystallize and DETERMINE the structure of as many proteins as possible, in many cases with little or no existing information about protein function The explanation is: Options b, C, d comes under the goals of HUMAN GENOMIC project.

Discussion

11.	The effects of protein on an entire organism is described in __________(a) Phenotypic function(b) Cellular function(c) Molecular function(d) Structural genomicsThis question was addressed to me in an internship interview.My question is based upon From Genomes to Proteasomes topic in section DNA-Based Information Technologies of Biochemistry
Answer» The CORRECT OPTION is (a) Phenotypic function Easiest explanation: Cellular function, it describes about the NETWORK of interactions engaged in by protein at cellular level. Molecular function, it describes the PRECISE BIOCHEMICAL activity of a protein. Structural genomics, it includes sequencing of genomic DNA.

Discussion

12.	Who was the first person to develop DNA finger printing?(a) David Suzuki(b) Khorana(c) Alec Jaffreys(d) GilbertThe question was asked in final exam.This is a very interesting question from From Genes to Genomes topic in section DNA-Based Information Technologies of Biochemistry
Answer» The CORRECT choice is (c) ALEC Jaffreys The EXPLANATION: Alec Jaffreys was the FIRST person to develop DNA finger printing.

Discussion

13.	Generally a plasmid vector contains how many elements?(a) 1(b) 2(c) 3(d) 4The question was asked during an online exam.I would like to ask this question from DNA Cloning: The Basics-1 in section DNA-Based Information Technologies of Biochemistry
Answer» The CORRECT ANSWER is (C) 3 The best explanation: Cloning site, DRUG resistant GENE and replication origin are the 3 elements in a plasmid vector.

Discussion

14.	The network of interactions engaged in by protein at cellular level is described in __________(a) Phenotypic function(b) Cellular function(c) Molecular function(d) Structural genomicsThis question was addressed to me by my college professor while I was bunking the class.This intriguing question comes from From Genomes to Proteasomes in division DNA-Based Information Technologies of Biochemistry
Answer» The correct option is (b) Cellular function The BEST I can EXPLAIN: Molecular function, it describes the precise BIOCHEMICAL activity of a protein. Structural genomics, it includes sequencing of GENOMIC DNA. Phenotypic function, it describes the effects of protein on an entire organism.

Discussion

15.	Recombinant DNA construction involves ____________(a) Cleaving DNA with a restriction endonuclease and joining with ligase(b) Cleaving DNA with ligase and joining with endonuclease(c) Cleaving and joining DNA with restriction endonuclease(d) Cleaving DNA with a restriction endonuclease and joining with polymeraseThis question was addressed to me in an interview for job.This question is from From Genes to Genomes topic in portion DNA-Based Information Technologies of Biochemistry
Answer» Right answer is (a) Cleaving DNA with a restriction endonuclease and joining with ligase Easiest EXPLANATION: Restriction ENZYMES cleave plasmid VECTOR and then DNA ligase JOINS FOREIGN DNA with cloning vector.

Discussion

16.	Electrophoresis helps to separate ____________(a) DNA segments(b) Cells from DNA(c) Tissues(d) RNA from DNAThe question was posed to me in examination.My enquiry is from From Genes to Genomes topic in division DNA-Based Information Technologies of Biochemistry
Answer» RIGHT ANSWER is (a) DNA segments To elaborate: In electrophoresis, DNA FRAGMENTS are separated ACCORDING to their SIZE.

Discussion

17.	Which of the following is a DNA element that instructs RNA polymerase where to bind on DNA?(a) Operator(b) Promoter(c) Enhancer(d) Hormone response elementThis question was posed to me at a job interview.My question is from DNA Cloning: The Basics in portion DNA-Based Information Technologies of Biochemistry
Answer» The correct choice is (b) Promoter For explanation: Promoter INSTRUCTS RNA POL where to bind on DNA.

Discussion

18.	E.cor1 is a __________(a) DNA ligase enzyme(b) Restriction endonuclease(c) A vector used for insulin synthesis(d) A plasmid used as a vectorThis question was addressed to me at a job interview.I need to ask this question from DNA Cloning: The Basics-1 topic in chapter DNA-Based Information Technologies of Biochemistry
Answer» Right answer is (b) Restriction endonuclease For explanation: Ecor1 is a restriction ENZYME that CLEAVES VECTOR at the CLONING site.

Discussion

19.	Sequencing of genomic DNA is included in __________(a) Phenotypic function(b) Cellular function(c) Molecular function(d) Structural genomicsI had been asked this question in exam.My enquiry is from From Genomes to Proteasomes in division DNA-Based Information Technologies of Biochemistry
Answer» CORRECT ANSWER is (d) Structural genomics For explanation I WOULD say: PHENOTYPIC function, it describes the effects of protein on an entire organism. Cellular function, it describes about the network of interactions engaged in by protein at cellular level. Molecular function, it describes the precise biochemical activity of a protein.

Discussion

20.	A set of overlapping DNA segments that together represent a consensus region of DNA is ____________(a) Expressed sequence tag(b) Sequence tagged site(c) Contig(d) YACThis question was addressed to me in an internship interview.My question is based upon From Genes to Genomes topic in chapter DNA-Based Information Technologies of Biochemistry
Answer» Right choice is (c) Contig The best I can explain: Sequence TAGGED site is a short DNA sequence having single occurrence in genome. Expressed sequence TAG is a short sub-sequence of a cDNA sequence. YAC is a LINEAR DNA molecule.

Discussion

21.	Site directed mutagenesis facilitated research on __________(a) Carbohydrates(b) Proteins(c) Lipids(d) FatsI have been asked this question during a job interview.This intriguing question originated from DNA Cloning: The Basics in chapter DNA-Based Information Technologies of Biochemistry
Answer» Correct answer is (b) Proteins For explanation: Site DIRECTED mutagenesis changes the amino acid sequence by ALTERING DNA sequence of cloned GENE. It facilitates RESEARCH on proteins.

Discussion

22.	In which stage of genetic engineering a probe is used?(a) Cleaving DNA(b) Recombining DNA(c) Cloning(d) ScreeningThe question was asked during an interview for a job.I'm obligated to ask this question of DNA Cloning: The Basics-1 topic in chapter DNA-Based Information Technologies of Biochemistry
Answer» CORRECT option is (d) SCREENING Easiest explanation: In hybridization, which is one of the METHODS of screening, a probe is used.

Discussion

23.	Which of the following temperature best suits for the growth and multiplication of transformed cells?(a) 35°C(b) 36°C(c) 37°C(d) 30°CI got this question in semester exam.My query is from DNA Cloning: The Basics in portion DNA-Based Information Technologies of Biochemistry
Answer» RIGHT option is (c) 37°C Explanation: (37-43) °C is the TEMPERATURE range PREFERRED for the GROWTH and multiplication of TRANSFORMED cells.

Discussion

24.	Production of a large number of genetically similar plants through plant tissue culture is called __________(a) Recombinant DNA technology(b) Gene therapy(c) Micro propagation(d) Hybridoma technologyThis question was addressed to me in an interview for internship.I need to ask this question from Genome Alterations and New Products of Biotechnology topic in portion DNA-Based Information Technologies of Biochemistry
Answer» CORRECT CHOICE is (c) Micro propagation The best explanation: Micro propagation is the practice of PRODUCING a LARGE number of genetically similar plants through plant TISSUE culture.

Discussion

25.	Explant can be a __________(a) Cut part of the plant used in tissue culture(b) Plant extract used in tissue culture(c) Source of growth regulators added to media(d) Solidifying agentThis question was posed to me in examination.Query is from Genome Alterations and New Products of Biotechnology topic in division DNA-Based Information Technologies of Biochemistry
Answer» CORRECT choice is (a) Cut part of the plant used in tissue CULTURE Easiest explanation: Explant is a piece of plant or ANIMAL tissue PLACED in a culture medium.

Discussion

26.	Which of the following term is associated with DNA finger printing?(a) Hybridoma(b) Site specific mutagenesis(c) Shotgun cloning(d) RFLPThis question was addressed to me in an interview for job.The question is from From Genes to Genomes in section DNA-Based Information Technologies of Biochemistry
Answer» Correct choice is (d) RFLP For explanation: RFLP technique is PREFERRED in FORENSIC LABORATORIES to identify the suspects.

Discussion

27.	Which of the following statements regarding DNA fingerprinting is false?(a) DNA fingerprinting cannot be used for paternity testing(b) DNA profile using STR (short terminal repeats) is unique to an individual(c) PCR is used for DNA profiling(d) Forensic analysis makes use of SNPs (single nucleotide polymorphisms) in coding sequences to distinguish between individualsI got this question during an internship interview.I'm obligated to ask this question of From Genes to Genomes in chapter DNA-Based Information Technologies of Biochemistry
Answer» CORRECT OPTION is (a) DNA fingerprinting cannot be USED for paternity TESTING The explanation: DNA fingerprinting is used for paternity testing.

Discussion

28.	Which of the following statements is false?(a) BAC is a circular DNA molecule(b) YAC is a linear DNA molecule(c) Taql is a DNA polymerase(d) BAC is a linear DNA moleculeThis question was addressed to me in exam.My doubt is from From Genes to Genomes in section DNA-Based Information Technologies of Biochemistry
Answer» Right OPTION is (d) BAC is a linear DNA molecule For explanation I would say: BAC is a CIRCULAR DNA molecule.

Discussion

29.	Which of the following statements is true about a vector?(a) Plasmids, phages can be used as vectors(b) All vectors are plasmids(c) Cyanobacteria can also be used as vectors(d) Fungi can also be used as vectorsI have been asked this question during a job interview.My doubt stems from DNA Cloning: The Basics topic in portion DNA-Based Information Technologies of Biochemistry
Answer» Right option is (a) Plasmids, PHAGES can be USED as VECTORS To EXPLAIN: Plasmids and phages can be used as vectors.

Discussion

30.	Which of the following is true about restriction endonucleases?(a) Type I and II requires ATP to move along DNA(b) Type I, II and III requires ATP to move along DNA(c) Type II requires no ATP and cleaves DNA within recognition sequence(d) Type II requires ATP and cleaves DNA within recognition sequenceThe question was posed to me in an internship interview.This is a very interesting question from DNA Cloning: The Basics-1 in portion DNA-Based Information Technologies of Biochemistry
Answer» Correct ANSWER is (c) Type II requires no ATP and cleaves DNA within RECOGNITION sequence Easy explanation: Type I and III requires ATP to move along DNA.

Discussion

31.	The study of the full complement of proteins expressed by a genome is called __________(a) Proteome(b) Proteomics(c) Genomics(d) Protein formationThis question was posed to me in class test.I need to ask this question from From Genomes to Proteasomes in chapter DNA-Based Information Technologies of Biochemistry
Answer» Correct ANSWER is (b) Proteomics For explanation: The FULL complement of proteins EXPRESSED by a genome is CALLED PROTEOME and the study of it is called proteomics.

Discussion

32.	The precise biochemical activity of a protein is described in __________(a) Phenotypic function(b) Cellular function(c) Molecular function(d) Structural genomicsI have been asked this question in a national level competition.This key question is from From Genomes to Proteasomes topic in portion DNA-Based Information Technologies of Biochemistry
Answer» Right ANSWER is (c) Molecular function The best I can EXPLAIN: STRUCTURAL genomics, it includes sequencing of genomic DNA. Phenotypic function, it DESCRIBES the effects of protein on an entire organism. Cellular function, it describes about the network of interactions engaged in by protein at cellular level.

Discussion

33.	Which of the following codons is not a termination codon for protein synthesis?(a) UUU(b) UAG(c) UAA(d) UGAThe question was asked by my school principal while I was bunking the class.This intriguing question comes from From Genes to Genomes-1 topic in portion DNA-Based Information Technologies of Biochemistry
Answer» The CORRECT ANSWER is (a) UUU To explain: After the RIBOSOME REACHES the codon UAG or UAA or UGA on m-RNA strand, protein synthesis is terminated.

Discussion

34.	Collection of microscopic DNA spots attached to the solid surface are __________(a) Ortholog(b) Synteny(c) Paralog(d) MicroarrayI got this question in a national level competition.Query is from From Genomes to Proteasomes topic in section DNA-Based Information Technologies of Biochemistry
Answer» Right ANSWER is (d) Microarray Easy EXPLANATION: Genes of different species but possessing a clear sequence and functional RELATIONSHIP to each other are orthologs. Genes of same species, similarly related to each other are paralogs. Synteny is a conserved gene ORDER.

Discussion

35.	A segment of DNA that reads from the same forward and backward is called ____________(a) Palindromic DNA(b) Complementary DNA(c) Plasmid DNA(d) Copy DNAI have been asked this question in a job interview.Origin of the question is From Genes to Genomes-1 in section DNA-Based Information Technologies of Biochemistry
Answer» The CORRECT ANSWER is (a) Palindromic DNA For explanation: A palindromic sequence is the same when read from 5’ to 3’ on one STRAND and 3’ to 5’ on its COMPLEMENTARY strand.

Discussion

36.	During DNA profiling, DNA nucleotides hybridized with the probe can be detected through ____________(a) Electrophoresis(b) Polymerase chain reaction(c) Autoradiography(d) HybridomaI have been asked this question in final exam.My question is based upon From Genes to Genomes in portion DNA-Based Information Technologies of Biochemistry
Answer» CORRECT OPTION is (c) Autoradiography To elaborate: Autoradiography is a DETECTION method in hybridization.

Discussion

37.	Which of the following enzyme is used in PCR?(a) Taq DNA polymerase(b) HRP(c) EcoRI(d) EcoRIIThe question was asked in exam.Question is taken from From Genes to Genomes-1 topic in chapter DNA-Based Information Technologies of Biochemistry
Answer» The correct ANSWER is (a) TAQ DNA polymerase To explain: HRP is an enzyme used in ELISA. EcorI and EcorII are the restriction enzymes. So only a DNA polymerase is responsible for producing multiple COPIES of DNA sequence.

Discussion

38.	Agar is extracted from ____________(a) Fungi(b) Bacteria(c) Algae(d) VirusThis question was addressed to me during a job interview.My query is from DNA Cloning: The Basics in chapter DNA-Based Information Technologies of Biochemistry
Answer» Correct option is (C) Algae Explanation: AGAR is obtained from an ALGA.

Discussion

39.	Which of the following can be termed as a restriction modification system?(a) Restriction endonuclease + methylase(b) DNA ligase + methylase(c) Restriction endonuclease + acetylase(d) DNA ligase + acetylaseThis question was posed to me in quiz.My question is taken from DNA Cloning: The Basics-1 topic in section DNA-Based Information Technologies of Biochemistry
Answer» Right OPTION is (a) RESTRICTION endonuclease + methylase To ELABORATE: (Restriction endonuclease + methylase) is termed as a restriction modification SYSTEM.

Discussion

40.	Conserved gene order can be termed as __________(a) Ortholog(b) Synteny(c) Paralog(d) MicroarrayThe question was posed to me in final exam.Enquiry is from From Genomes to Proteasomes topic in section DNA-Based Information Technologies of Biochemistry
Answer» Right answer is (b) Synteny The best I can explain: GENES of DIFFERENT species but possessing a clear sequence and functional relationship to each other are orthologs. Genes of same species, similarly related to each other are paralogs. Collections of microscopic DNA spots attached to SOLID surface are DNA MICROARRAYS.

Discussion

41.	cDNA, a term used in recombinant DNA technology means ____________(a) Competitive DNA(b) Chemical DNA(c) Complex DNA(d) Complementary DNAThe question was posed to me by my school principal while I was bunking the class.This intriguing question comes from From Genes to Genomes-1 topic in portion DNA-Based Information Technologies of Biochemistry
Answer» Right answer is (d) COMPLEMENTARY DNA Explanation: Complementary DNA is ALSO TERMED as cDNA.

Discussion

42.	Which of the following endonuclease removes a length of DNA between two telomere sequences?(a) EcoR1(b) EcoR2(c) BamHI(d) HindIIII got this question during a job interview.Enquiry is from DNA Cloning: The Basics topic in division DNA-Based Information Technologies of Biochemistry
Answer» Right CHOICE is (C) BAMHI The explanation is: BamHI removes a LENGTH of DNA between two telomere sequences.

Discussion

43.	Which of the following enzyme is required for end to end joining of DNA?(a) DNA ligase(b) Restriction endonuclease(c) RNA polymerase(d) DNA polymeraseThe question was asked in an online interview.Question is taken from DNA Cloning: The Basics-1 topic in chapter DNA-Based Information Technologies of Biochemistry
Answer» The CORRECT option is (a) DNA LIGASE The best explanation: DNA ligase ligates both blunt and sticky ENDS of DNA.

Discussion

44.	To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?(a) MgCl2(b) CaCl2(c) NaCl(d) HClI have been asked this question in my homework.This intriguing question originated from DNA Cloning: The Basics-1 in portion DNA-Based Information Technologies of Biochemistry
Answer» Right CHOICE is (b) CACL2 To elaborate: The CELLS are treated with CaCl2 to make them permeable to DNA molecules through a process called TRANSFORMATION.

Discussion

45.	Generally, YAC contains how many elements?(a) 1(b) 2(c) 3(d) 4The question was posed to me during an interview.My doubt is from DNA Cloning: The Basics topic in section DNA-Based Information Technologies of Biochemistry
Answer» Right option is (d) 4 To EXPLAIN: Ori, selectable markers X and Y, 2 TELOMERES and 1 centromere.

Discussion

46.	Recombinant DNA molecule is produced by ligating __________(a) Two DNA segments(b) Two m-RNA molecules(c) One m-RNA with one t-RNA(d) One m-RNA with DNA segmentI have been asked this question in an interview for internship.Question is from DNA Cloning: The Basics in section DNA-Based Information Technologies of Biochemistry
Answer» The CORRECT answer is (a) Two DNA segments The explanation is: For the CONSTRUCTION of recombinant DNA molecule, two DNA segments are LIGATED.

Discussion

47.	Which of the following is not denatured by heating steps during PCR?(a) DNA pol(b) RNA pol(c) Restriction enzymes(d) DNA ligaseThe question was posed to me during an internship interview.This interesting question is from From Genes to Genomes topic in portion DNA-Based Information Technologies of Biochemistry
Answer» The CORRECT answer is (a) DNA pol Explanation: DNA polymerase, Taq is THERMO stable, so it is not denatured by heating steps during PCR.

Discussion

48.	Meristem culture helps in developing which of the following?(a) Tall plants(b) Disease resistant plants(c) Virus free plants(d) Hybrid plantsI had been asked this question in an internship interview.The doubt is from Genome Alterations and New Products of Biotechnology topic in chapter DNA-Based Information Technologies of Biochemistry
Answer» The correct answer is (c) Virus free plants The BEST explanation: APICAL meristem from the infected PLANT is COLLECTED and cultivated in APPROPRIATE media so that new, complete and virus-free plants develop.

Discussion

49.	What is a DNA library?(a) A DNA fragment inserted into a vector(b) A general collection of all genes sequenced thus far(c) All DNA fragments identified with a probe(d) A collection of DNA fragments that make up the entire genome of a particular organismThis question was posed to me in semester exam.This is a very interesting question from DNA Cloning: The Basics-1 topic in chapter DNA-Based Information Technologies of Biochemistry
Answer» Correct choice is (d) A COLLECTION of DNA fragments that make up the entire GENOME of a particular organism Best EXPLANATION: DNA library is a collection of DNA fragments REPRESENTING the entire DNA of an organism.

Discussion

50.	Which of the following does not act as a restriction enzyme?(a) EcorI(b) BamHI(c) HindIII(d) polydeoxyribonucleotide synthaseI got this question by my school principal while I was bunking the class.My question comes from DNA Cloning: The Basics-1 topic in portion DNA-Based Information Technologies of Biochemistry
Answer» CORRECT choice is (d) polydeoxyribonucleotide synthase To explain I would say: Polydeoxtribonucleotide synthase is a DNA ligase.

Discussion

Explore topic-wise InterviewSolutions in .

Which of the following is helpful in distinguishing DNA of one individual from another?(a) PCR(b) Reverse transcriptase(c) cDNA(d) RFLPThis question was posed to me in an online interview.This question is from From Genes to Genomes-1 in portion DNA-Based Information Technologies of Biochemistry

Who was the first person to develop DNA finger printing?(a) David Suzuki(b) Khorana(c) Alec Jaffreys(d) GilbertThe question was asked in final exam.This is a very interesting question from From Genes to Genomes topic in section DNA-Based Information Technologies of Biochemistry

Generally a plasmid vector contains how many elements?(a) 1(b) 2(c) 3(d) 4The question was asked during an online exam.I would like to ask this question from DNA Cloning: The Basics-1 in section DNA-Based Information Technologies of Biochemistry

Electrophoresis helps to separate ____________(a) DNA segments(b) Cells from DNA(c) Tissues(d) RNA from DNAThe question was posed to me in examination.My enquiry is from From Genes to Genomes topic in division DNA-Based Information Technologies of Biochemistry

Sequencing of genomic DNA is included in __________(a) Phenotypic function(b) Cellular function(c) Molecular function(d) Structural genomicsI had been asked this question in exam.My enquiry is from From Genomes to Proteasomes in division DNA-Based Information Technologies of Biochemistry

Site directed mutagenesis facilitated research on __________(a) Carbohydrates(b) Proteins(c) Lipids(d) FatsI have been asked this question during a job interview.This intriguing question originated from DNA Cloning: The Basics in chapter DNA-Based Information Technologies of Biochemistry

In which stage of genetic engineering a probe is used?(a) Cleaving DNA(b) Recombining DNA(c) Cloning(d) ScreeningThe question was asked during an interview for a job.I'm obligated to ask this question of DNA Cloning: The Basics-1 topic in chapter DNA-Based Information Technologies of Biochemistry

Which of the following temperature best suits for the growth and multiplication of transformed cells?(a) 35°C(b) 36°C(c) 37°C(d) 30°CI got this question in semester exam.My query is from DNA Cloning: The Basics in portion DNA-Based Information Technologies of Biochemistry

Which of the following term is associated with DNA finger printing?(a) Hybridoma(b) Site specific mutagenesis(c) Shotgun cloning(d) RFLPThis question was addressed to me in an interview for job.The question is from From Genes to Genomes in section DNA-Based Information Technologies of Biochemistry

Collection of microscopic DNA spots attached to the solid surface are __________(a) Ortholog(b) Synteny(c) Paralog(d) MicroarrayI got this question in a national level competition.Query is from From Genomes to Proteasomes topic in section DNA-Based Information Technologies of Biochemistry

Which of the following enzyme is used in PCR?(a) Taq DNA polymerase(b) HRP(c) EcoRI(d) EcoRIIThe question was asked in exam.Question is taken from From Genes to Genomes-1 topic in chapter DNA-Based Information Technologies of Biochemistry

Agar is extracted from ____________(a) Fungi(b) Bacteria(c) Algae(d) VirusThis question was addressed to me during a job interview.My query is from DNA Cloning: The Basics in chapter DNA-Based Information Technologies of Biochemistry

Conserved gene order can be termed as __________(a) Ortholog(b) Synteny(c) Paralog(d) MicroarrayThe question was posed to me in final exam.Enquiry is from From Genomes to Proteasomes topic in section DNA-Based Information Technologies of Biochemistry

Which of the following endonuclease removes a length of DNA between two telomere sequences?(a) EcoR1(b) EcoR2(c) BamHI(d) HindIIII got this question during a job interview.Enquiry is from DNA Cloning: The Basics topic in division DNA-Based Information Technologies of Biochemistry

Generally, YAC contains how many elements?(a) 1(b) 2(c) 3(d) 4The question was posed to me during an interview.My doubt is from DNA Cloning: The Basics topic in section DNA-Based Information Technologies of Biochemistry