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Which of the following statements is true?(a) Exonuclease III removes nucleotide residues from the 3’ ends of a DNA strand(b) Bacteriophage lambda exonuclease removes terminal phosphates(c) Alkaline phosphatase removes nucleotides from 5‘ends(d) Kinase adds homopolymer tails to the 3’ –OH ends of a linear duplexI had been asked this question during an online interview.Origin of the question is DNA Cloning: The Basics-1 in chapter DNA-Based Information Technologies of Biochemistry

Answer»

Correct answer is (a) Exonuclease III removes NUCLEOTIDE residues from the 3’ ends of a DNA strand

Explanation: BACTERIOPHAGE lambda exonuclease removes nucleotides from 5‘ends.

Alkaline phosphatase removes terminal phosphates

Terminal transferase ADDS homopolymer TAILS to the 3’ –OH ends of a linear duplex.



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