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Recombinant DNA construction involves ____________(a) Cleaving DNA with a restriction endonuclease and joining with ligase(b) Cleaving DNA with ligase and joining with endonuclease(c) Cleaving and joining DNA with restriction endonuclease(d) Cleaving DNA with a restriction endonuclease and joining with polymeraseThis question was addressed to me in an interview for job.This question is from From Genes to Genomes topic in portion DNA-Based Information Technologies of Biochemistry

Answer»

Right answer is (a) Cleaving DNA with a restriction endonuclease and joining with ligase

Easiest EXPLANATION: Restriction ENZYMES cleave plasmid VECTOR and then DNA ligase JOINS FOREIGN DNA with cloning vector.



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