InterviewSolution
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InterviewSolution
This section includes InterviewSolutions, each offering curated multiple-choice questions to sharpen your knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Crystal of Bt toxin produced by some bacteria do not kill the bacteria becauseA. Bacteria are resistant to the toxinB. Toxin is inactiveC. Toxin is immatureD. Bacteria enclose toxin is a special sac. |
| Answer» Correct Answer - B | |
| 2. |
Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves becauseA. Bacteria are resistant to the toxinB. Toxin is immatureC. Toxin is inactiveD. Bacteria enclose the toxin in special sac. |
| Answer» Correct Answer - C | |
| 3. |
Which technique is used in separating fragments of DNAA. Eastern blottingB. Western blottingC. Northern blottingD. Southern blotting |
| Answer» Correct Answer - D | |
| 4. |
Endonucleases break DNA at specific sites calledA. Palindromic sequencesB. Restriction sitesC. Conserved sitesD. Both A and B |
| Answer» Correct Answer - D | |
| 5. |
Select the wrong statementA. Presence of chromogenic substrate gives blue colour colonies if the plasmid in the bacteria does not have an insertB. Retroviruses in animals have the ability to transform normal cells into cancerous cellsC. In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNAD. Since DNA is a hydrophilic molecule it cannot pass through cell membranes |
| Answer» Correct Answer - C | |
| 6. |
Fragments of DNA formed after treatment with endonucleases are separated by the techniqueA. Polymerase chain reactionB. Southern blottingC. Colony hybridisationD. Electrophoresis |
| Answer» Correct Answer - D | |
| 7. |
Select the wrong statementA. Human insulin is being commercially produced from a transgenic species of Escherichia coliB. Genetically modified Bacillus thuringiensis is used as biopesticide on commercial scaleC. Human protein, `alpha`-1-antitrypsin, is used to treat emphysemaD. Bt toxin genes cry IAC control corn borer |
| Answer» Correct Answer - D | |
| 8. |
In insectional inactivation of `beta`-galactosidase gene, the bacteria in white colonies haveA. Nonrecombinant plasmidB. Recombinant plasmidC. No plasmidD. Linear foreign DNA |
| Answer» Correct Answer - B | |
| 9. |
Restriction endonucleases areA. Synthesized by bacteria as part of their defence mechanismB. Used in vitro DNA synthesisC. Present in mammalian cells to degrade DNA when the cells dieD. Used in genetic engineering for ligation of two DNA molecules. |
| Answer» Correct Answer - A | |
| 10. |
DNA fragments generated by restriction endonucleases in a chemical reaction can be separated byA. Restriction mappingB. CentrifugationC. Polymerase chain reactionD. Agarose gel electrophoresis |
| Answer» Correct Answer - D | |
| 11. |
Restriction endonucleases are called so as theyA. Synthesize DNAB. Restrict nuclear activityC. Cleave DNA into fragmentsD. Break DNA at random. |
| Answer» Correct Answer - C | |
| 12. |
Restriction endonucleases are enzymes thatA. Restrict the action of other enzymesB. Break phosphodiester bond between specific nucleotides of DNAC. Break nucleus into piecesD. Break H-bond between two strands of DNA |
| Answer» Correct Answer - B | |
| 13. |
Human insulin is being commercially produced from transgenic species ofA. E. coliB. Brassica napusC. Bacillus thuringiensisD. Agrobacterium |
| Answer» Correct Answer - A | |
| 14. |
Frost resistance is obtained from genetically engineeredA. Escherichia coliB. Pseudomonas putidaC. Pseudomonas fluorescenceD. Trichoderma |
| Answer» Correct Answer - C | |
| 15. |
Bt cotton is preferred over other varieties of cotton because of itsA. High yieldB. Quick growthC. Weedicidal propertyD. Resistance to bollworm |
| Answer» Correct Answer - D | |
| 16. |
Transgenic pigs with genes for human antigens will be useful forA. Providing best human foodsB. Resistance to common human diseaseC. Having all important blood factorsD. Organ transplantation |
| Answer» Correct Answer - D | |
| 17. |
Pseudomonas putida cleansA. Oils spillsB. SewageC. HidesD. None of the above |
| Answer» Correct Answer - A | |
| 18. |
Bioremediation isA. Genetically engineered organisms capable of producing pharmaceuticalsB. Biological controlC. Biopurification of environmentD. Both B and C |
| Answer» Correct Answer - C | |
| 19. |
Taq polymerase used in polymerase chain reaction is active at temperature ofA. `25^(@) - 30^(@)C`B. `35^(@) - 50^(@)C`C. `50^(@) - 70^(@)C`D. `72^(@) - 90^(@)C` |
| Answer» Correct Answer - D | |
| 20. |
The technique used to make numerous copies of a specific segment of DNA quickly and accurately isA. Ligase chain reactionB. TranscriptionC. Polymerase chain reactionD. Translation |
| Answer» Correct Answer - C | |
| 21. |
In PCR, Taq polymerase is used betweenA. Extraction and denaturationB. Denaturation and annealingC. Annealing and extensionD. Extension and amplification |
| Answer» Correct Answer - C | |
| 22. |
Maximum number of existing transgenic animals is of:A. CowB. PigC. MiceD. Fish |
| Answer» Correct Answer - C | |
| 23. |
Agarose extracted from sea weeds finds use inA. PCRB. Gel electrophoresisC. SpectrophtometryD. Tissue culture |
| Answer» Correct Answer - B | |
| 24. |
Read statements a-d. Which two of them have mistakes (a) First transgenic buffalo Roise produced milk which was human alpha-lactalbumin enriched (b) Restriction enzymes are used in isolation of DNA from other macromolecules (c) Downstream processing is one of the steps of rDNA technology (d) Disarmed pathogen vectors are also used in transfer of rDNA into the hostA. b and cB. c and dC. a and cD. a and b |
| Answer» Correct Answer - D | |
| 25. |
The introduction of t-DNA into plants involvesA. Infection of the plant by Agrobacterium tumefaciensB. Altering the pH of the soil, then heat shocking the plantsC. Exposing the plants to cold for a brief periodD. Allowing the plant roots to stand in water. |
| Answer» Correct Answer - A | |
| 26. |
Introduction of transgenes wil result inA. Formation of a new speciesB. Formation of a new proteinC. Alter a biosynthetic pathwayD. Both B and C |
| Answer» Correct Answer - D | |
| 27. |
Cyanogen bromide is employed inA. Genetic finger printingB. Tissue cultureC. Synthesis of humulinD. Hybridoma technology |
| Answer» Correct Answer - C | |
| 28. |
In transgenics, the expression of transgene in the target tissue is known byA. EnhancerB. TransgeneC. PromoterD. Reporter |
| Answer» Correct Answer - D | |
| 29. |
An abnormal gene is replaced by normal gene. It is calledA. Gene therapyB. CloningC. MutationD. None of the above |
| Answer» Correct Answer - A | |
| 30. |
Transgenic animals are those which haveA. Foreign DNA in some of its cellsB. Foreign DNA in all its cellsC. Foreign RNA in all its cellsD. DNA and RNA both in the cells |
| Answer» Correct Answer - B | |
| 31. |
Bollworms attacksA. Bt cottonB. TomatoC. CottonD. Bacillus thuringiensis |
| Answer» Correct Answer - C | |
| 32. |
The two polypeptides of human insulin are linked together byA. Disulphide bridgesB. Hydrogen bondsC. Phosphodiester bridgeD. Covalent bond. |
| Answer» Correct Answer - A | |
| 33. |
Identify the correct sequence of steps in RNA-interference (RNAi) process to develop nematode resistant plants (i) Silencing of specific mRNA of the nematode (ii) Formation of sense and antisense RNA (iii) dsRNA formation (iv) Introducing nematode specific genes into plantA. i, ii, iv, iiiB. iii, ii, i, ivC. iv, ii, iii, iD. iv, i, ii, iii |
| Answer» Correct Answer - C | |
| 34. |
Basic principle for developing transgenic plants and animals is to introduce the gene of interest into nucleus ofA. Body cellB. Vegetative cellC. Germ cellD. Somatic cell |
| Answer» Correct Answer - C | |
| 35. |
What is true of Bt toxin ?A. The concentrated bacillus has antitoxinsB. The inactive protoxin gets converted into active form in the insect gutC. Bt protein exists as active toxin in the bacillusD. The activated toxin enters the ovaries of the pest to sterilize it and this prevents its multiplication. |
| Answer» Correct Answer - B | |
| 36. |
Transgenic plants are plants havingA. No geneB. Genes in transpositionC. Genes have no function to performD. Genes of an other organism |
| Answer» Correct Answer - D | |
| 37. |
Transgenic plants are developed byA. Introducing foreign genesB. Introducing gene mutationsC. Deleting certain chromosome partsD. Stopping spindle formation. |
| Answer» Correct Answer - A | |
| 38. |
Transgenic plants are the onesA. Grown on artificial medium after hybridisation in the fieldB. Produced after protoplast fusion in artificial mediumC. Produced by somatic embryo on artificial mediumD. Generated by introducing foreign DNA into a cell and regenerating plant from that cell. |
| Answer» Correct Answer - D | |
| 39. |
Extrachromosomal DNA used as vector in gene cloning isA. TransposonB. IntronC. ExonD. Plasmid |
| Answer» Correct Answer - D | |
| 40. |
Which is used in production of insulin by genetic engineeringA. RhizobiumB. SaccharomycesC. MycobacteriumD. Escherichia |
| Answer» Correct Answer - D | |
| 41. |
Hybridomas are employed forA. Production of somatic hybridsB. Killing cancer cellsC. Synthesis of antibioticsD. Synthesis of monoclonal antibodies |
| Answer» Correct Answer - D | |
| 42. |
The first recombinant DNA was constructed by linking an antibiotic resistant gene with the native plasmid ofA. Escherichia coliB. Salmonella typhimuriumC. Clostridium butylicumD. Acetobacter aceti |
| Answer» Correct Answer - B | |
| 43. |
Which one is cloning plasmid, not an expression plasmidA. pBAD - 18 - CamB. pBCSKC. pUC 18D. pET |
| Answer» Correct Answer - C | |
| 44. |
In gel electrophoresis, differential mobility of DNA depends uponA. Helical nature of DNAB. Double stranded nature DNAC. Charge and size of DNAD. Hydrogen bonding between bases |
| Answer» Correct Answer - C | |
| 45. |
Find the correct match A. a-iii-p, b-v-s, c-ii-t, d-i-rB. a-i-s, b-iv-p, c-ii-t, d-iii-qC. a-iii-p, b-v-s, c-i-r, d-iv-qD. a-iii-p, b-iv-q, c-i-r, d-ii-t |
| Answer» Correct Answer - C | |
| 46. |
Select DNA sequence which would act as a restriction siteA. `(A A C C G G)/(T T G G C C )`B. `(G G T T G G)/(C C A A C C)`C. `(A A G G C T)/(T T C C G A)`D. `(C T G C A G)/(G A C G T C)` |
| Answer» Correct Answer - D | |
| 47. |
Which one of the following statements is not correct about a plasmidA. It is a circular DNAB. It has antibiotic resistant geneC. It has the ability of autonomousD. Its DNA is as long as chromosomal DNA |
| Answer» Correct Answer - D | |
| 48. |
Which one is correct answer for plasmid (i) It can be readily isolated from cells (ii) It possesses a single recognition site for one restriction enzyme (iii) It should have high molecular weight (iv) Plasmids and bacteriophages have the ability to replicate within bacteriaA. i, ii, ivB. i, ii, iii, ivC. ii, iii, ivD. i, ii, iii |
| Answer» Correct Answer - A | |
| 49. |
Genetic engineering isA. Plastic surgeryB. Addition or removal of genesC. Study of extra nuclear genesD. All the above |
| Answer» Correct Answer - B | |
| 50. |
Cloning is means toA. Replace original genotypeB. Preserve genotypeC. Production of HGH gene in Escherichia coliD. None of the above |
| Answer» Correct Answer - B | |