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Competence can be generated in some strains of non-competent bacteria by __________(a) Treating then with Chitinase(b) By treating them with penicillin(c) By electroporation(d) By pentrifugationThis question was posed to me in class test.I would like to ask this question from Gene Mapping in Bacteria by Transformation in portion Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

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The correct answer is (C) By electroporation

The explanation: Electroporation is a TECHNIQUE where a short pulse F ELECTRICITY is used to induce artificial competence in some cells. Treating with chitinase or penicillin will SEVERELY affect the cell wall and the cells will die.



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