In and interrupted mating experiment, it is seem that the after 10 mins Azi^R gene in recipient, after 20 minuites there is appearance of ton^Rand after 15 mins the recipient is lac^+ , but the recipient is almost never his^+.Where in the genome is the ori of transfer?(a) Between Azi^R and Ton^R genes(b) Between Ton^R and Lac^+(c) Between Lac^+ and His^+(d) Between His^+ and Azi^RI had been asked this question during an interview for a job.This is a very interesting question from Gene Mapping in Bacteria by Conjugation topic in section Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

In and interrupted mating experiment, it is seem that the after 10 min

1.	In and interrupted mating experiment, it is seem that the after 10 mins Azi^R gene in recipient, after 20 minuites there is appearance of ton^Rand after 15 mins the recipient is lac^+ , but the recipient is almost never his^+.Where in the genome is the ori of transfer?(a) Between Azi^R and Ton^R genes(b) Between Ton^R and Lac^+(c) Between Lac^+ and His^+(d) Between His^+ and Azi^RI had been asked this question during an interview for a job.This is a very interesting question from Gene Mapping in Bacteria by Conjugation topic in section Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
Answer» Right ANSWER is (d) Between His^+ and Azi^R The best EXPLANATION: As the origin of TRANSFER is between His^+ and Azi^R the sequence follows Azi^R, ->Ton^R->Lac^+->His^+ or the reverse direction. Thus, longer TIME is required for His to enter and in most cases before that the conjugation tube breaks.

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