How do we measure the concentration of the enzyme?(a) In units of mass(b) In units of time(c) In units of activity(d) In units of volumeThis question was posed to me in an interview for internship.This interesting question is from Basic Reaction Theory in section Homogenous Reactions of Bioprocess Engineering

How do we measure the concentration of the enzyme?(a) In units of mass

1.	How do we measure the concentration of the enzyme?(a) In units of mass(b) In units of time(c) In units of activity(d) In units of volumeThis question was posed to me in an interview for internship.This interesting question is from Basic Reaction Theory in section Homogenous Reactions of Bioprocess Engineering
Answer» The correct option is (c) In units of activity To elaborate: The mass of a particular enzyme added to a reaction is rarely known; most commercial enzyme PREPARATIONS contain SEVERAL components in unknown and variable proportions depending on the BATCH obtained from the manufacturer. To overcome these difficulties, enzyme quantity is often expressed as units of activity measured under specified conditions. One unit of enzyme is usually taken as the amount which catalyses conversion of 1μmol substrate per minute at the optimal TEMPERATURE, pH and substrate concentration.

Discussion

No Comment Found

Related InterviewSolutions

What is the growth condition for Saccharomyces cerevisiae with glucose as energy source?(a) Aerobic(b) Anaerobic(c) Tryptophan limited(d) Glucose limitedThe question was posed to me in semester exam.Question is from Kinetics of Cell Substrate Uptake in Cell Culture topic in portion Homogenous Reactions of Bioprocess Engineering
The monod equation is based on which type of kinetics?(a) Zero order kinetics(b) First-order kinetics(c) Second order kinetics(d) First-zero order kineticsThe question was asked during a job interview.I'd like to ask this question from Kinetics of Cell Substrate Uptake in Cell Culture topic in division Homogenous Reactions of Bioprocess Engineering
A homogenous material is defined as being ____________(a) An element(b) Any material with uniform composition(c) Synonymous with “solution”(d) Any material with non-uniform compositionThe question was posed to me by my school teacher while I was bunking the class.My question is taken from Effect of Maintenance on Yields in section Homogenous Reactions of Bioprocess Engineering
Consider the growth of a microorganism in batch culture. When the substrate concentration is high, the cell density doubles every 0.75 h, the observed substrate yield coefficient is 0.3 g DCW/g, and substrate consumption is allocated towards biosynthesis (60%), maintenance (10%), as well as product formation (30%). The product formation is strictly growth-associated. The batch reactor is inoculated with 0.01 g DCW/L and 10 g/L substrate. Estimate the maximum cell density (after lag phase).(a) 1.21 g DCW / l(b) 1.41 g DCW / l(c) 1.61 g DCW / l(d) 1.81 g DCW / lI got this question during a job interview.This interesting question is from Effect of Maintenance on Yields topic in division Homogenous Reactions of Bioprocess Engineering
The biomass concentration is at the highest level in which phase?(a) Lag phase(b) Log phase(c) Exponential phase(d) Stationary phaseThe question was asked in examination.Query is from Kinetics of Cell Substrate Uptake in Cell Culture topic in division Homogenous Reactions of Bioprocess Engineering
The supply of substrate for production and maintenance is different.(a) True(b) FalseI have been asked this question in final exam.I'm obligated to ask this question of Kinetics of Cell Substrate Uptake in Cell Culture in division Homogenous Reactions of Bioprocess Engineering
A completely mixed continuous stirred-tank reactor for the cultivation of cells is called?(a) Turbidostat(b) Chemostat(c) Haemostat(d) ThermostatThis question was addressed to me in homework.I want to ask this question from Kinetics of Cell Substrate Uptake in Cell Culture topic in section Homogenous Reactions of Bioprocess Engineering
Logistic growth model describes which type of growth?(a) Product- based growth(b) Non-growth(c) Substrate-based growth(d) Maintenance-based growthI had been asked this question in an online interview.The origin of the question is Kinetics of Cell Substrate Uptake in Cell Culture in division Homogenous Reactions of Bioprocess Engineering
The Theoretical yield is higher than the observed yield.(a) True(b) FalseThis question was addressed to me during an online exam.My query is from Production Kinetics in Cell Culture topic in section Homogenous Reactions of Bioprocess Engineering
Which of the following production kinetics is produced at the same time as cell growth?(a) Growth associated(b) Non-growth associated(c) Mixed-growth associated(d) Variable- growth associatedThis question was posed to me in an international level competition.My question is based upon Production Kinetics in Cell Culture topic in division Homogenous Reactions of Bioprocess Engineering

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment