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In a particular enzyme-catalyzed reaction, Vmax = 0.2 mol/sec and Km = 5 mM. Assume the enzyme shows standard Michaelis-Menten kinetics. What is the rate of the reaction when [S] = 10 mM?(a) 0.133(b) 0.331(c) 0.233(d) 0.332I had been asked this question during an interview.This key question is from General Reaction Kinetics for Biological Systems topic in section Homogenous Reactions of Bioprocess Engineering

Answer»

The CORRECT CHOICE is (a) 0.133

Easiest explanation: v = VMAX[S] / (KM + [S])

= 0.2×10 / (5+10)

= 0.133.



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