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In Ames test, the bacterial culture are grown in _________(a) Natural media(b) Blood agar(c) Enriched media(d) Minimal mediaI got this question in semester exam.The above asked question is from DNA Mutation in portion DNA Mutation, DNA Repair, Transposable Elements of Cytogenetics |
Answer» CORRECT option is (d) MINIMAL MEDIA For explanation I would say: Only prototroph can grow in minimal media. In the Ames test, the mutagenicity of a substance is tested by its ability to convert an auxotroph into a prototroph. THUS to check for mutants, the media must be minimal so that only mutant COLONIES grow. |
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