In an experiment you want to express your gene of interest in a prokaryote through a plasmid. What would be your ideal copy number?(a) 2 or 3(b) 10(c) 80(d) 100The question was asked in quiz.The above asked question is from Plasmids topic in chapter Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

In an experiment you want to express your gene of interest in a prokar

1.	In an experiment you want to express your gene of interest in a prokaryote through a plasmid. What would be your ideal copy number?(a) 2 or 3(b) 10(c) 80(d) 100The question was asked in quiz.The above asked question is from Plasmids topic in chapter Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
Answer» The correct answer is (a) 2 or 3 To elaborate: The GENE of interest by us should always be expressed in low copy number as if it is in higher copy number t will use more of bacterial recourses without helping them at all. So, BACTERIA MIGHT then DISPENSE some of these chromosomes.

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