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Km is directly proportional to the rate of enzyme binding?(a) True(b) FalseI got this question during an interview for a job.The question is from Determining Enzyme Kinetic Constants from Batch Data in portion Homogenous Reactions of Bioprocess Engineering

Answer»

The CORRECT answer is (b) False

Easy explanation: Km reflects the enzyme’s dissociation constant. A high Km means WEAK binding (the enzyme LIKES to dissociate from its SUBSTRATE), and a low Km means strong binding (it doesn’t LIKE to dissociate from its substrate, meaning that it has a strong affinity for the substrate).



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