Microcarrier beads 120 μm in diameter are used to culture recombinant CHO cells for production of growth hormone. It is proposed to use a 6-cm turbine impeller to mix the culture in a 3.5-1itre stirred tank. Air and carbon dioxide are supplied by flow through the reactor headspace. The microcarrier suspension has a density of approximately 1010 kg m^-3 and a viscosity of 1.3 x 10^-3 Pa s. Estimate the kinematic viscosity.(a) 1.30 × 10^-6 m^2s^-1(b) 1.29 × 10^-6 m^2s^-1(c) 1.50 × 10^-6 m^2s^-1(d) 1.49 × 10^-6 m^2s^-1This question was posed to me by my school principal while I was bunking the class.The query is from Role of Shear in Stirred Fermenters in portion Fluid Flow and Mixing of Bioprocess Engineering

Microcarrier beads 120 μm in diameter are used to culture recombinant

1.	Microcarrier beads 120 μm in diameter are used to culture recombinant CHO cells for production of growth hormone. It is proposed to use a 6-cm turbine impeller to mix the culture in a 3.5-1itre stirred tank. Air and carbon dioxide are supplied by flow through the reactor headspace. The microcarrier suspension has a density of approximately 1010 kg m^-3 and a viscosity of 1.3 x 10^-3 Pa s. Estimate the kinematic viscosity.(a) 1.30 × 10^-6 m^2s^-1(b) 1.29 × 10^-6 m^2s^-1(c) 1.50 × 10^-6 m^2s^-1(d) 1.49 × 10^-6 m^2s^-1This question was posed to me by my school principal while I was bunking the class.The query is from Role of Shear in Stirred Fermenters in portion Fluid Flow and Mixing of Bioprocess Engineering
Answer» Right option is (b) 1.29 × 10^-6 m^2s^-1 Easiest explanation: Damage due to eddies is avoided if the \(K \ddot{o}\) 1mogorov scale remains greater than 2/3-1/2 the diameter of the beads. LET US determine the stirrer speed required to create eddies with size, λ = 2/3 (120 μm) = 80 μm = 8 x 10^-5 RN. The stirrer power producing eddies of this dimension can be estimated: Kinematic viscosity, v = \(\frac{\MU}{\rho} = \frac{1.3×10^{-3} kg \,m^{-1} s^{-1}}{1010 \,kgm^{-3}}\) = 1.29 × 10^-6 m^2s^-1.

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