Restriction enzymes cleave the bond by which type of reaction?(a) SN1(b) SN2(c) SE1(d) SE2The question was asked in a national level competition.My question is taken from Restriction Endonuclease in portion Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

Restriction enzymes cleave the bond by which type of reaction?(a) SN1(

1.	Restriction enzymes cleave the bond by which type of reaction?(a) SN1(b) SN2(c) SE1(d) SE2The question was asked in a national level competition.My question is taken from Restriction Endonuclease in portion Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
Answer» The correct ANSWER is (B) SN2 For explanation I WOULD SAY: The reaction is by hydrolysis of the phosphodiester bond between two sugar residues by a nucleophilic attack. Inverted product configuration shows that the substrate was inverted only once. As only bimolecular nucleophilic reaction causes only one time inversion so that is the correct option.

Discussion

No Comment Found

Related InterviewSolutions

In EcoRV the enzyme is a ____________(a) Homo dimer(b) Hetero dimer(c) Trimer(d) Hetero tetramerI got this question by my college director while I was bunking the class.Question is from Restriction Endonuclease topic in portion Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
What does this sequence GC/GGCCGC recognized and cleaves by Not1 Doesn’t agree to?(a) The cleavage is after the 2^nd C from 5’ end(b) The cleavage generates a 5’ overhang(c) This sequence has a probability of occurrence in every 1024 bases(d) This cleavage is asymmetricThe question was posed to me by my college professor while I was bunking the class.The above asked question is from Restriction Endonuclease in chapter Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
A specialized transducing phage attacking an infected cell with lysogenic stage of the same virus gives rise to________(a) HFR(b) HFT(c) F’(d) FI got this question in a national level competition.Enquiry is from Gene Mapping in Bacteria by Transduction in chapter Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
Which of the following viruses will be useful for transduction?(a) T2 phage(b) T4 phage(c) T7 phage(d) Lamda phageThis question was posed to me in final exam.My enquiry is from Gene Mapping in Bacteria by Transduction in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
In and interrupted mating experiment, it is seem that the after 10 mins Azi^R gene in recipient, after 20 minuites there is appearance of ton^Rand after 15 mins the recipient is lac^+ , but the recipient is almost never his^+.Where in the genome is the ori of transfer?(a) Between Azi^R and Ton^R genes(b) Between Ton^R and Lac^+(c) Between Lac^+ and His^+(d) Between His^+ and Azi^RI had been asked this question during an interview for a job.This is a very interesting question from Gene Mapping in Bacteria by Conjugation topic in section Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
Bacteriocins are _______________(a) Toxic nucleic acids(b) Non-ribosomally synthesized toxic proteins(c) Ribosomally synthesized toxic proteins(d) Modified normal proteinsThe question was asked during an internship interview.My doubt stems from Plasmids topic in portion Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
In an experiment you introduce your gene of interest in a wild type E. cloli genome using an episome. However, you fail to see the product protein being formed. What could be the possible reason for this?(a) The gene was silenced(b) The gene product was produced in negligible quantity(c) The bacteria was in stress so it was not producing much protein(d) The gene was excised outThe question was asked in an international level competition.This intriguing question originated from Restriction Endonuclease in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
Which of the following is not true about type II restriction endonuclease?(a) Here Clevage and Methylation are by different enzymes(b) In this case the recognition sequence is asymmetric(c) It cleaves within the recognition sequence(d) It can’t cleave at methylated sequencesThe question was posed to me during a job interview.The query is from Restriction Endonuclease in chapter Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
You are checking the sequence of 3 genes using generalized transduction mapping. The recipient is Leu-,Azi^S, Thr- while the donor is Leu+,Azi^R, Thr +. If you select the Leu+ as marker,Azi^R would be 50% while Thr+ would be 2%. If you select Thr+ as marker Leu+ would be 3% and Azi^R would be 0%. What is the sequence?(a) Thr Leu Azi(b) Azi Thr Leu(c) Azi Leu Thr(d) Thr Azi LeuThis question was addressed to me in an online interview.My enquiry is from Gene Mapping in Bacteria by Transduction in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
In a transduction experiment where the recipient is Met-, Ala-, Gal- you observe a recombinant phenotype Met+, Gal+ in some strains and Gal+ Ala+ in some strains. But in none of the cases can you find Met+ Ala+. What is the sequence of the gene?(a) Met, Ala, Gal(b) Met, Gal, Ala(c) Gal, Met, Ala(d) Gal, Ala, MetThis question was posed to me in semester exam.My question comes from Gene Mapping in Bacteria by Transduction topic in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

Restriction enzymes cleave the bond by which type of reaction?(a) SN1(b) SN2(c) SE1(d) SE2The question was asked in a national level competition.My question is taken from Restriction Endonuclease in portion Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

Discussion

No Comment Found

Related InterviewSolutions

Reply to Comment