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The final step involves pairwise alignment by extending from the words in both directions while counting the alignment score using the same substitution matrix.(a) True(b) FalseI have been asked this question during an interview for a job.My question is based upon Basic Local Alignment Search Tool (BLAST) topic in portion Database Similarity Searching of Bioinformatics |
Answer» RIGHT answer is (a) True Easy explanation: The extension continues until the score of the alignment drops below a threshold due to mismatches (the drop threshold is twenty-two for PROTEINS and twenty for DNA). The resulting contiguous aligned segment pair without GAPS is called high-scoring segment pair. In the ORIGINAL version of BLAST, the highest scored HSPs are presented as the FINAL report. They are also called maximum scoring pairs. |
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