The Mg^2+ ion in restriction endonuclease is not bound to ________(a) Water molecule(b) Carboxylates of the enzymes aspertate group(c) Oxygen(d) HistidineI got this question in a national level competition.This question is from Restriction Endonuclease topic in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

The Mg^2+ ion in restriction endonuclease is not bound to ________(a)

1.	The Mg^2+ ion in restriction endonuclease is not bound to ________(a) Water molecule(b) Carboxylates of the enzymes aspertate group(c) Oxygen(d) HistidineI got this question in a national level competition.This question is from Restriction Endonuclease topic in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
Answer» CORRECT ANSWER is (d) Histidine The explanation is: In restriction endonucleases we don’t have His as a LIGAND to the central metal ion Mg^2+. While it’s binding to the other GROUPS assists in the nucleophilic attack.

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