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What is the term “Km”?(a) Concentration of the enzyme(b) Concentration of the catalyst(c) Concentration of the product(d) Concentration of the substrateI had been asked this question in final exam.Enquiry is from Determining Enzyme Kinetic Constants from Batch Data in section Homogenous Reactions of Bioprocess Engineering

Answer» RIGHT option is (d) Concentration of the substrate

Best explanation: For practical purposes, Km is the concentration of substrate which permits the enzyme to ACHIEVE HALF vmax. An enzyme with a high Km has a low affinity for its substrate, and REQUIRES a GREATER concentration of substrate to achieve vmax.


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