Which of the following enzyme air is a neoisoschizomer?(a) SphI and BbuI(b) Not1 and HindIII(c) SmaI and XmaI(d) Pst1 and Sma1This question was posed to me during an internship interview.The origin of the question is Restriction Endonuclease topic in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

Which of the following enzyme air is a neoisoschizomer?(a) SphI and Bb

1.	Which of the following enzyme air is a neoisoschizomer?(a) SphI and BbuI(b) Not1 and HindIII(c) SmaI and XmaI(d) Pst1 and Sma1This question was posed to me during an internship interview.The origin of the question is Restriction Endonuclease topic in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics
Answer» Correct OPTION is (c) SMAI and XmaI Best EXPLANATION: Neo-isoschizomers are the enzymes that RECOGNIZE the same sequence and cleave at different points. Here, both SMA1 and Xma1 recognize GGGCCC; the 1^st cleaving after the 3^rd G and 2^nd cleaving after the 1^st.

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