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Which of the following is incorrect regarding Cluster-Buster?(a) It is an HMM-based web-based program(b) A query sequence is scanned with a window size of 1 kb for putative regulatory motifs using motif HMMs(c) It works by detecting a region of high concentration of unknown transcription factor binding sites and regulatory motifs at the initiation(d) It is designed to find clusters of regulatory binding sitesI got this question by my school principal while I was bunking the class.The doubt is from Prediction Algorithms topic in division Promoter and Regulatory Element Prediction of Bioinformatics

Answer»

Right answer is (c) It works by detecting a region of high concentration of unknown transcription factor binding sites and regulatory motifs at the initiation

To EXPLAIN: It works by detecting a region of high concentration of known transcription factor binding sites and regulatory motifs. If multiple motifs are detected within a window, a positive score is assigned to each motif found. The TOTAL score of the window is the SUM of each motif score SUBTRACTING a gap penalty, which is proportional to the distances between motifs. If the score of a certain region is above a certain threshold, it is predicted to contain a regulatory cluster.



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