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Which of the following is not a chemical method to measure kLa?(a) Dynamic gassing out method(b) Sodium sulfite oxidation method(c) Carbon dioxide absorption method(d) Glucose oxidase methodThis question was addressed to me during an online exam.My doubt stems from Measurement of KLa topic in portion Mass Transfer of Bioprocess Engineering

Answer»

Right option is (a) Dynamic gassing out method

For explanation: This method HINGES on the measurement of the DISSOLVED OXYGEN concentration that is altered by absorption or desorption, facilitated by flushing with inert gases like nitrogen. The instantaneous dissolved oxygen concentration can be measured by using electrodes and kLa is hence ESTIMATED from the slope of the resulting PLOT.



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