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Which of the following is not a protein denaturant?(a) SDS(b) Phenol(c) DTT(d) Acetic acidThe question was posed to me in quiz.I'd like to ask this question from Types of Biological Molecules topic in portion Structures and Functions of Biological Molecules of Cell Biology

Answer»

The correct option is (d) ACETIC acid

To explain I would SAY: Sodium dodecyl sulfate or SDS is a strong denaturant of protein and is used in SDS-PAGE technique. DTT is OFTENTIMES used along with SDS to further denature proteins by reducing their disulfide bonds to ALLOW for better separation of proteins during electrophoresis. Phenol is used as a protein denaturant during isolation of RNA from plants.



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