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Which of the following state represents the condition – “Initial mixing of E+S, while [ES] builds up”.(a) Enzyme is saturated with substrate(b) Pre- Steady state(c) Steady – state(d) Steady-state kineticsThe question was asked by my school teacher while I was bunking the class.Query is from Determining Enzyme Kinetic Constants from Batch Data in portion Homogenous Reactions of Bioprocess Engineering

Answer»

Right choice is (b) PRE- Steady state

Best explanation: In the first moment after an ENZYME is MIXED with substrate, no PRODUCT has been formed and no INTERMEDIATES exist. The study of the next few milliseconds of the reaction is called pre-steady-state kinetics.



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