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Which of the sequence will Dcm methylate?(a) GATC(b) GAATC(c) CCGTT(d) CCAGGI had been asked this question in an online interview.This interesting question is from Restriction Endonuclease in section Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

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Right option is (d) CCAGG

Easy explanation: DCM methylates at the C base while DAM methylates at the A base. DCM methylates TA the internal C of the sequence CCAGG. The sequence CCGTT is not a palindrome and GATC is recognized and methylated by DAM.



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