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Which type of enzymes is used in DNA footprinting?(a) RNA-digesting enzymes(b) RNA polymerases(c) DNA polymerases(d) DNA-digesting enzymesI got this question in an international level competition.I'm obligated to ask this question of Gene Expression Control in chapter Control of Gene Expression of Cell Biology

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Correct answer is (d) DNA-digesting enzymes

For explanation: DNA-digesting enzymes are used in DNA footprinting to determine the genetic sequences that are PROTECTED by transcription factors. The basic principle of the technique is that when transcription factors bind to a PARTICULAR sequence, it protects the sequence from DIGESTION by nucleases.



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