Explore topic-wise InterviewSolutions in .

a) Those organisms which contains functional foreign gene. 

b) Produced by the introduction of foreign gene in tomato plant which delay ripening and offers high shelf life. Thus it helps in reducing post harvest losses.Flavr Savr Tomato was created by Agrobacterium mediated gene transformation in which the transfer of T DNA contained a copy of tomato PG (polygalacturonase) encoding gene in the antisense orientation 

c) He is concerned about his friends welfare and making his friend aware of the new techniques for delaying the ripening of tomato.

Agrobacterlum tumejaciens

Correct answer is (b) Agrobacterium tumtfaciens

Pachytene stage of prophase I by crossing over.

Due to the presence of cell wall in plants cellulose is used, whereas the animal cell lacks cell wall.

EcoRI comes from the name of bacterium Escherechia coli Ry 13. E represents the genus, co represents species, ‘R’ is derived from the name of strain and I is the order in which the enzyme is isolated.

(a) direct gene transfer

1. The working of programmed cylinder for PCR requires high temperature . Hence thermostable DNA poly merase is used

2. Taq polymerase

(b) Salmonella typhimurium

1. Slicing enzyme – Restriction endo nuclease Splicing enzyme-DNA ligase

2. significance of slicing enzyme in rDNA technology: 

• It is used to cut within DNA sequence.
• Same restriction enzyme is used to cut both vector DNA and foreign DNA.

Separation and purification of gene product.

(a) DNA ligase

(b) Agrobacterium tumefaciens

(b) homozygote obtained asexually

Agrobacterium tumifaciens.

(c) It has the ability to carry a foreign gene

1. Gel electrophoresis

2. Separation of DNA fragments

1. Herbert Boyer and Stanley cohen constructed first recombinant DNA by linking a gene encoding antibiotic resistance with a native plasmid of Salmonella typhimurium in 1972.

2. Restriction endonuclease

(b) Agrobacterium tumefaciens

The name given to it is Plasmid.

Methodology used:

• Sequence Annotation – total DNA from a cell is isolated, converted into random fragments of relatively smaller sizes, and cloned in suitable host using specialized vectors.

• The cloning resulted into amplification of each piece of DNA fragment.

• The fragments were sequenced using automated DNA sequencers, these sequences are then arranged based on some overlapping regions (present in them).

• This requires generation of overlapping fragments (for sequencing).

• Specialised computer based programmes were developed, and these sequences were subsequently annotated and assigned to each chromosome.

3’OH group is absent in ddNTP’s which cause termination of growing DNA chain during Sanger’s DNA sequencing method.

DNA fragments formed by chain termination in all the four tubes for the given strand 3’ ATGCTAGC 5'

3’OH group is absent in ddNTP’s which cause termination of growing DNA chain during Sanger’s DNA sequencing method.

DNA fragments formed by chain termination in all the four tubes for the given strand . 3’ ATGCTAGC 5’